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pGEX2T-TEV CHMP1B 1-143

Plasmid

#108286

Purpose

Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-143

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_SPAG9_SKAP1

Plasmid

#205925

Purpose

Express mEGFP-tagged fusion protein, SPAG9_SKAP1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

SPAG9_SKAP1 (SPAG9, SKAP1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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AAV pTRE-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65449

Purpose

Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Promoter

tetO

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-flex-Voltron-ST

Plasmid

#119036

Purpose

Cre-dependant expression of soma-localized Voltron in neurons

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Has Service

AAV1

Insert

Voltron-ST

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hsyn

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING(pLxIS)

Plasmid

#221240

Purpose

Expression of STING CTT in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING CTT (Sting1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC13P-iCLHN

Plasmid

#66580

Purpose

CLYBL safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-C13 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Nanoluc-HaloTag

Expression

Mammalian

Promoter

CAG

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-T131A

Plasmid

#213482

Purpose

Expresses human SKP1 T131A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-T131A (SKP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT POLI

Plasmid

#131228

Purpose

Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Coding sequence has been codon optimised for expr…

Promoter

CMV enhancer + CMV promoter

Available Since

Dec. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEx1-pEF-H2B-mCherry-T2A-rTetR-EED

Plasmid

#78101

Purpose

Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-EED (EED Human, Synthetic)

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

Plasmid

#165082

Purpose

gRNA 1 of pair B for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-Ago2

Plasmid

#72207

Purpose

Mammalian expression of full-length human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING(FL)

Plasmid

#221243

Purpose

Expression of STING without transmembrane domain in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING without transmembrane domain (Sting1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+FLAG-KrasnatG12D

Plasmid

#206842

Purpose

To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Aspartic Acid

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_NCOA4_RET

Plasmid

#205865

Purpose

Express mEGFP-tagged fusion protein, NCOA4_RET from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

NCOA4_RET (NCOA4, RET Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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JAM2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176966

Purpose

Mammalian expression vector encoding JAM2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

JAM2 (JAM2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgCtrl#1

Plasmid

#174142

Purpose

Lentiviral vector expressing a control sgRNA that targets a safe harbor site

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo-RL-ZEB1 200bmutx5

Plasmid

#35537

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB1 (ZEB1 Human, Budding Yeast)

Use

Luciferase

Expression

Mammalian

Mutation

5 mutations in miR200b seed regions of 3'UTR…

Available Since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry

Plasmid

#231007

Purpose

Protein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

E4F1 aa220-242 (E4F1 Human)

Tags

AcGFP1

Expression

Mammalian

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_sgCtrl#2

Plasmid

#174149

Purpose

Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX Grb2-SH2

Plasmid

#46440

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb2 (GRB2 Human)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 58-159

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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