We narrowed to 8,785 results for: sgrna

1. pSEM255 - sgRNA mosTI Pmlc-2_GFP

   Plasmid

   #159835

   Purpose

   Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI Pmlc-2_GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti pTERT sgRNA1(MS2) mCherry

   Plasmid

   #171054

   Purpose

   Targeting of Vp64-dCas9 activation system to hTERT promoter

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Insert

   pTERT sgRNA1(MS2) mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

   Plasmid

   #47550

   Purpose

   Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Inserts

   Cas9
   ttTi5605 sgRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   U6 and eef-1A.1 (eft-3)

   Available Since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

   Plasmid

   #124537

   Purpose

   Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   d11-41 C55G, d11-41 C55G

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry

   Plasmid

   #177183

   Purpose

   plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   sgRNA targeting the PEAR-GFP plasmid
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX601-miniCMV-SaCas9-U6-LacZvsSaCas9 sgRNA

   Plasmid

   #107049

   Purpose

   All-in-one vectors expressing both SaCas9 and LacZ sgRNA

   Depositor

   Alex Hewitt

   Insert

   SaCas9
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

   Plasmid

   #70658

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

8. lenti-GBA-N370S-nick sgRNA

   Plasmid

   #214096

   Purpose

   Lentiviral vector expressing nicking sgRNA to induce GBA N370S mutation using PE3

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   KRAS G13A nicking sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

   Plasmid

   #71829

   Purpose

   Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   BACH2-sgRNA8 (BACH2 Human, Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E756A inactiva…

   Promoter

   U6

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. peSpCas9(1.1)-2×sgRNA (IFT88, donor)

    Plasmid

    #80769

    Purpose

    Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Insert

    IFT88 gRNA#1 (IFT88 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLG1-puro Non-targeting sgRNA 3

    Plasmid

    #109003

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdCas9+sgRNA expression vector precursor

    Plasmid

    #171798

    Purpose

    PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

    Depositor

    Stephen Elledge

    Article

    Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO

    Available Since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-pMecp2-SaCas9-pU6-sgRNA

    Plasmid

    #159173

    Purpose

    Vector A encodes pAAV-pMecp2-SaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of SaCas9-sgRNA in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pMecp2

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

    Plasmid

    #162335

    Purpose

    Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    S. pyogenes sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL1 sgRNA(MS2)-MS2-AIDmono

    Plasmid

    #112127

    Purpose

    empty sgRNA cloning vector with MS2-AIDmono

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    AID mono mutant (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    N- mutated and C- terminal truncated, H130A/R131E…

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

    Plasmid

    #159283

    Purpose

    A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Inserts

    Human INS sgRNA
    Human INS guide RNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti-EGFR-L858R-nick sgRNA

    Plasmid

    #214097

    Purpose

    Lentiviral vector expressing nicking sgRNA to induce EGFR L858R mutation using PE3

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    EGFR L858R nicking sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-EGFR-T790M-nick sgRNA

    Plasmid

    #214098

    Purpose

    Lentiviral vector expressing nicking sgRNA to induce EGFR T790M mutation using PE3

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    EGFR T790M nicking sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLH-sgRNA1-boxB-MS2-PP7

    Plasmid

    #75397

    Purpose

    sgRNA1-boxB-MS2-PP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-boxB-MS2-PP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomere

    Plasmid

    #85714

    Purpose

    Telomere-targeting Nme sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    telomere sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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