We narrowed to 6,128 results for: nls
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Plasmid#133394PurposePiggybac vector for heterologous promoter reporter assay of human ASCL2 promoterDepositorInsertdestabilized tdTomato-NLS and destabilized NLS-GFP
UseTagsExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free) drives expressi…Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-FGFR4
Plasmid#60531PurposeEntry vector for human FGFR4DepositorInsertFGFR4 (FGFR4 Human)
UseEntry vectorTagsExpressionMutationc2134a P712TPromoterNAAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMKO shRNA Bim
Plasmid#17235DepositorInsertBim shRNA (BCL2L11 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP
Plasmid#171090PurposeCRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.DepositorInsertsSth1 dCas9
Chloramphenicol acetyltransferase
TagBFP
UseCRISPR; Toxoplasma gondii expressionTags3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…ExpressionMutationD9A, H599APromoterTUB1 (Toxoplasma gondii), transcriptionally linke…Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_WT
Plasmid#98657PurposeExpresses 6xHis-tagged hnRNPA2 AA 190-341DepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB-USP7
Plasmid#99393PurposeMammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7DepositorInsertUSP7 (USP7 Human)
UseTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pST1374-GCN4-D10A
Plasmid#113022PurposeExpresses GCN4-D10A in mammalian cellsDepositorInsertGCN4-D10A (GCN4 Budding Yeast, S. pyogenes)
UseTagsExpressionMammalianMutationD10APromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
BE3-P2A-EGFP (pJUL977)
Plasmid#123612PurposeCAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.DepositorInsertBE3-P2A-EGFP
UseTagsExpressionMammalianMutationD10A in SpCas9PromoterCAGAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-APOBEC-nCas9-Ung
Plasmid#198552PurposeExpresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-APOBEC-nCas9-Ung
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_nocharge
Plasmid#139118Purposeexpress hnRNPA2 LC with no charged residuesDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall charged residues are changed to S/Q except fo…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
dCas9-DNMT3L
Plasmid#126588PurposeDNMT3L fused to C-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertDNMT3L (DNMT3L Human)
UseCRISPRTags3XFLag-NLS-dCas9-DNMT3L-NLSExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS1859
Plasmid#205972PurposeFLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCsy3/Cas7 from JAGBLH010000220, Cas8-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1860
Plasmid#205973PurposeFLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCas6/Csy4 from JAGBLH010000220, Cas8-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9
Plasmid#84244PurposeExpresses GA-inducible VPR-Sa dCas9DepositorInsertsGAI-tagBFP-Sa dCas9
GID1-VPR
UseCRISPR and Synthetic Biology; PiggybacTagsGAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …ExpressionMammalianMutationD10A, N580A and Synonymous mutation in PYL1 to re…PromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
eA3A-BE3 (pRZ206)
Plasmid#131315PurposeCAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.DepositorInserthA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-IFT54
Plasmid#218726PurposeExpresses N-terminally EGFP-tagged IFT54 in mammalian cellsDepositorInsertIFT54 (TRAF3IP1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
SECURE miniABEmax-K20A/R21A (pJUL1774)
Plasmid#131312PurposeCMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).DepositorInsertbpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe HA human Bmf
Plasmid#17239DepositorInsertBmf (BMF Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
hA3A-BE3 (pRZ215)
Plasmid#131314PurposeCAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.DepositorInserthA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST1374-D10A-GCN4
Plasmid#113025PurposeExpresses D10A-GCN4 in mammalian cellsDepositorInsertD10A-GCN4 (GCN4 Budding Yeast, S. pyogenes)
UseTagsExpressionMammalianMutationD10APromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST1374-GCN4-dCas9
Plasmid#113023PurposeExpresses GCN4-dCas9 in mammalian cellsDepositorInsertGCN4-dCas9 (GCN4 Budding Yeast, S. pyogenes)
UseTagsExpressionMammalianMutationD10A, D839A, H840A, N863APromoterCMVAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
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StrKDEL-IRES-mannosidase II-mTagBFP2
Plasmid#165460PurposeExpressed mannII-BFP simultaneously with a RUSH Hook Str-KDEL.DepositorInsertPartial ManII-mTag2BFP fusion (Man2a1 Mouse)
UseTagsStr-KDEL and mTag2BFPExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
hAID-BE3 (pRZ352)
Plasmid#131316PurposeCAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.DepositorInserthAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
VRQR-HF1 variant
Plasmid#138563PurposeExpresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSDDepositorInsertVRQR-HF1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…PromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_LC
Plasmid#98661PurposeExpresses MBP-tagged hnRNPA2 AA 190-341DepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1
Plasmid#101177PurposeHuman expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-eSpCas9(1.1)-HF1(N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationN497A/R661A/Q695A/K848A/Q926A/K1003A/R1060APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pST1374-dCas9-GCN4
Plasmid#113026PurposeExpresses dCas9-GCN4 in mammalian cellsDepositorInsertdCas9-GCN4 (GCN4 Budding Yeast, S. pyogenes)
UseTagsExpressionMammalianMutationD10A, H840A, N863APromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_deltaLC
Plasmid#98664PurposeExpresses MBP-tagged hnRNPA2 without the LC domainDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTags6xHis-MBPExpressionBacterialMutationG190StopPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_FL_D290V
Plasmid#98663PurposeExpresses MBP-tagged full length hnRNPA2 with D290VDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTags6xHis-MBPExpressionBacterialMutationD290VPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3xFLAG human Bmf
Plasmid#17242DepositorInsertBmf (BMF Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_FL_P298L
Plasmid#104468Purposeexpress MBP-tagged full length hnRNPA2 P298LDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsHis-MBPExpressionBacterialMutationP298LPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
TC1464
Plasmid#164881PurposeCMV-bpNLS-dCas13d-bpNLS-ADARdd, ADARdd embeded in loop1DepositorInsertdcas13d-ADARdd
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3xFLAG mouse Bmf
Plasmid#17243DepositorInsertBmf (Bmf Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pST1374-GCN4-H840A
Plasmid#113024PurposeExpresses GCN4-H840A in mammalian cellsDepositorInsertGCN4-H840A (GCN4 Budding Yeast, S. pyogenes)
UseTagsExpressionMammalianMutationD839A, H840A, N863APromoterCMVAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST1374-H840A-GCN4
Plasmid#113027PurposeExpresses H840A-GCN4 in mammalian cellsDepositorInsertH840A-GCN4 (GCN4 Budding Yeast, S. pyogenes)
UseTagsExpressionMammalianMutationD839A, H840A, N863APromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
TET1CD-CRY2-EGFP
Plasmid#82555PurposeEncodes TET1 under LITE2.0 systemDepositorUseAAVTags2A, NLS(alpha-imp), and NLS-VP64ExpressionMammalianMutationCRY2PHR (N-terminal photolyase homology region do…PromoterEF-1alphaAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe human Bmf
Plasmid#17240DepositorInsertBmf (BMF Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_P298L_S285C
Plasmid#104469Purposeexpress His tagged P298L S285C hnRNPA2 LCDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsHisExpressionBacterialMutationP298L S285CPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRY-GalVP16l (B694)
Plasmid#92030Purposeexpresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)DepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsGalBD-VP16ExpressionMammalianMutationNLS sequence of CRY2 mutatedPromoterCMVAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe mouse Bmf
Plasmid#17241DepositorInsertBmf (Bmf Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
yTREX-ApR
Plasmid#177307Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5DepositorTypeEmpty backboneUseSynthetic Biology; Yeast expression, tn5 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW21K_1Ti1
Plasmid#177291Purposegenomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and eYFPDepositorInserteYFP
UseSynthetic Biology; Yeast expression, rrn genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW22K_7Ti1
Plasmid#177293Purposegenomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and LacZDepositorInsertlacZ
UseSynthetic Biology; Yeast expression, rrn genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW27K_7Ti1
Plasmid#177294Purposegenomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and LacZDepositorInsertPsacB-sacB
UseSynthetic Biology; Yeast expression, rrn genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTSK56K_3G7
Plasmid#177301Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and mCherryDepositorInsertmCherry
UseSynthetic Biology; Yeast expression, tn7 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTSK58K_6G7
Plasmid#177302Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and PE-HDepositorInsertpe-h
UseSynthetic Biology; Yeast expression, tn7 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTSK65K_8G7
Plasmid#177303Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and GUSDepositorInsertuidA
UseSynthetic Biology; Yeast expression, tn7 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW07K_0G5
Plasmid#177279Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmRDepositorInsertPaacC1-aacC1
UseSynthetic Biology; Yeast expression, tn5 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW08K_0C5
Plasmid#177280Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with CmRDepositorInsertPcat-cat
UseSynthetic Biology; Yeast expression, tn5 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only