We narrowed to 7,818 results for: Lif

1. acta1-mRuby2-dGBP

   Plasmid

   #163855

   Purpose

   Zebrafish muscle specific expression of mRuby2-dVHH[GFP]

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   dVHH[GFP]
   

   Use

   In vitro transcription of rna

   Tags

   Ruby2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. acta1-mRuby2-VHH[GFP]

   Plasmid

   #163856

   Purpose

   Zebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRX

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   VHH[GFP]
   

   Use

   In vitro transcription of rna

   Tags

   Ruby2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT3TS-CD44b-TurboID

   Plasmid

   #163850

   Purpose

   In vitro transcription of CD44b-TurboID. Parton lab clone JTJ

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   CD44b (cd44b Zebrafish)
   

   Use

   In vitro transcription of rna

   Tags

   TurboID

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

   Plasmid

   #202569

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Use

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Arginine 261 to Alanine

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMJ915

   Plasmid

   #69090

   Purpose

   Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

   Depositor

   Jennifer Doudna

   Article

   Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

   Insert

   cas9
   

   Use

   Tags

   MBP, hexa His, and two SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. P336-FBP_11

   Plasmid

   #139702

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   various

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p323-L10A-PATagRFP

   Plasmid

   #74172

   Purpose

   Fluorescent reporter for single ribosome tracking

   Depositor

   Robert Singer

   Article

   Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

   Insert

   ribosomal protein L10A (Rpl10a Rat)
   

   Use

   Lentiviral

   Tags

   PATagRFP

   Expression

   Mutation

   Promoter

   UbC

   Available since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEBTet_NADP-Snifit-cyto

   Plasmid

   #113917

   Purpose

   Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NADP-Snifit
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV TetO2

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-SEP-GluA1 TKIT

   Plasmid

   #169441

   Purpose

   Expression of 2 guides + donor DNA

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-eGFP-TAX1BP1

    Plasmid

    #175766

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST_6xUAS:mNeonGreen-Rab5a

    Plasmid

    #192355

    Purpose

    zfRabaa tagged with mNeonGreen, expressed under the UAS promoter

    Depositor

    Francesca Peri

    Article

    Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

    Insert

    Rab5a (rab5aa Zebrafish)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV.CAG.GLPLight1

    Plasmid

    #187467

    Purpose

    Expresses GLPLight1 in mammalian cells

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

    Insert

    GLPLight1
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTAMAHISTEV_BauA

    Plasmid

    #128952

    Purpose

    expresses BauA in E.coli

    Depositor

    James Naismith

    Article

    Moynie et al Elife. 2018 Dec 18;7. pii: 42270. doi: 1

    Insert

    ferric acinetobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-66)

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFlpStop-attB-UAS-2.1-tdTom

    Plasmid

    #88910

    Purpose

    attB FlpStop plasmid, for insertion into MiMIC site

    Depositor

    Tom Clandinin

    Article

    Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

    Insert

    FlpStop
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    5XUAS

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Cyto-GCaMP3

    Plasmid

    #64853

    Purpose

    Cytosol-targeted version of the Ca2+ indicator GCaMP3.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    GCaMP3
    

    Use

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEMHE-H2B-mEGFP

    Plasmid

    #105528

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged Histon 2B mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    H2B (H2BC11 Human)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR iRFP-ZtSH2

    Plasmid

    #188627

    Purpose

    Fluorescent reporter for CD3ε/ZtSH2 pYtag biosensor

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    ZtSH2
    

    Use

    Lentiviral

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TRE-DIO-hM3Dq-mCherry

    Plasmid

    #166599

    Purpose

    Encodes Cre-dependent hM3Dq-mCherry under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Puro-T2A-mCitrine-hSmad2-donor

    Plasmid

    #203663

    Purpose

    Donor plasmid for CRISPR knock-in

    Depositor

    Ali Hemmati Brivanlou

    Article

    Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

    Insert

    Smad2
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZLCv2-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106357

    Purpose

    Empty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitation

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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