163,398 results

1. pCMV-BE4-SsAPOBEC3B

   Plasmid

   #138343

   Purpose

   Mammalian expression plasmid for BE4-SsAPOBEC3B

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-SsAPOBEC3B
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR.CMV

   Plasmid

   #32688

   Purpose

   Mammalian expression and gateway entry vector

   Depositor

   Ming-Sound Tsao

   Article

   Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Frt-V5-hspB8

   Plasmid

   #63109

   Purpose

   Expression of HSPB8 (small HSP) tagged with V5 in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb8 (HSPB8 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR- flag-Lag16-Ecad

   Plasmid

   #205197

   Purpose

   This vector encodes of the synCAM (Ecad) and GFP nanobody LAG16

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM Ecad and Lag16 anti-GFP nanobody
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-APX(W41F)-NES

   Plasmid

   #42608

   Depositor

   Alice Ting

   Article

   Rhee et al Science. 2013 Jan 31.

   Insert

   soybean APX W41F (dimeric) -NES
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   W41F mutation

   Promoter

   CMV

   Available since

   March 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-VPS15-TSF

   Plasmid

   #203563

   Purpose

   Mammalian expression of VPS15 with TwinStrep-Flag tag

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   VPS15 (PIK3R4 Human)
   

   Use

   Tags

   TwinStrep Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEYFP-N1-HsSmg6_H

   Plasmid

   #146475

   Purpose

   Mammalian Expression of HsSmg6

   Depositor

   Elisa Izaurralde

   Article

   Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

   Insert

   HsSmg6 (SMG6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations T594C, A699G and two non sil…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKIIa-hM4D(Gi)-mCherry (AAV2)

   Viral Prep

   #50477-AAV2

   Purpose

   Ready-to-use AAV2 particles produced from pAAV-CaMKIIa-hM4D(Gi)-mCherry (#50477). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM4D(Gi)-mCherry plasmid DNA. CaMKIIa-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   CaMKII

   Tags

   mCherry

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pWZL Neo Myr Flag ILK

   Plasmid

   #20505

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   ILK (ILK Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pHR-flag-GFP-JAM-B

    Plasmid

    #205191

    Purpose

    This vector encodes of a synCAM (JAM-B) and GFP

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM JAM-B and GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAG-AausFP1-Z1

    Plasmid

    #135047

    Purpose

    AausFP1 - bright green fluorescent protein expression from A. australis under CAG promoter in minimal backbone plasmid.

    Depositor

    Jordan Green

    Insert

    AausFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG Promoter

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLC-HA-Nedd8-P2A-Hygro

    Plasmid

    #124306

    Purpose

    Lentiviral vector for expression of HA tagged Nedd8-P2A-Hygro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    Nedd8 (NEDD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGilesSHdI_psmyc_His6_triple red

    Plasmid

    #170047

    Purpose

    Mono-Responsive Reporter Gene Constructs for triple red

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mScarlet
    COmScarlet
    mRuby3
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-MS2-HA-HshnRNPA2-B1_Z

    Plasmid

    #148113

    Purpose

    Mammalian Expression of HshnRNPA2-B1

    Depositor

    Elisa Izaurralde

    Insert

    HshnRNPA2-B1 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    splicing variant of hnRNPA2 variant B1. Exon 6 an…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSF11-wmiRFP713-T2A-HO1

    Plasmid

    #197246

    Purpose

    Expresses the protein of wmiRFP713-T2A-HO1 in neurons of C. elegans

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmiRFP713-T2A-HO1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. (114) pcDNA3.1-GFP-Flag-JunB-EPEA

    Plasmid

    #160743

    Purpose

    Expresses a GFP tagged JunB with Flag and EPEA tags.

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    GFP-Flag-JunB-EPEA (JUN Human)
    

    Use

    Tags

    EPEA, Flag, and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p11d-sctRPA

    Plasmid

    #102614

    Purpose

    Expression of S. cerevisiea RPA in E.coli

    Depositor

    Marc Wold

    Article

    Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

    Inserts

    RPA1 (RFA1 Budding Yeast)
    RPA2 (RFA2 Budding Yeast)
    RPA3 (RFA3 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    RFA2 has intron deleted

    Promoter

    T7

    Available since

    Oct. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-flag-GFP-MUC4

    Plasmid

    #205189

    Purpose

    This vector encodes of a synCAM (MUC4) and GFP

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM MUC4 and GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Frt-hspB5

    Plasmid

    #63096

    Purpose

    Expression of HSPB5 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb5 (CRYAB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. VRK1_HUMAN_D0

    Plasmid

    #79684

    Purpose

    This plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

21. qMaLioffG

    Plasmid

    #212825

    Purpose

    green fluorescent FLIM indicator for endogenous ATP imaging

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al bioRxiv 2023.08.29.555275

    Insert

    qMaLioffG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA5FRT PUR mGSK3AWT

    Plasmid

    #35159

    Depositor

    Jim Woodgett

    Article

    Doble et al Dev Cell. 2007 Jun;12(6):957-71.

    Insert

    GSK-3α (Gsk3a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

23. pMK312 (TOP2A CRISPR)

    Plasmid

    #140654

    Purpose

    TOP2A tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti.CMV/TO_PRKAA2

    Plasmid

    #74447

    Purpose

    Lentiviral expression vector encoding untagged WT human AMPK alpha 2

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    AMPK alpha 2 (PRKAA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pGEX nsp1 CoV2

    Plasmid

    #175512

    Purpose

    For protein expression of WT nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHH0103_GNAI1_G-alpha

    Plasmid

    #110012

    Purpose

    Protein expression and purification of GNAI1_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNAI1_G-alpha (GNAI1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EZ-L560

    Plasmid

    #173028

    Purpose

    Encodes OptoAMP2 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDule-3-nitroTyrosine (5B)

    Plasmid

    #85498

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PB-CMV-NLS-dCas9-NLS

    Plasmid

    #99908

    Purpose

    For the expression of NLS-dCas9-NLS

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Insert

    NLS-dCas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-MCS:

    Plasmid

    #179585

    Purpose

    ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette (Red module-dsRed/Green module-NeonGreen)

    Depositor

    Ali Hemmati Brivanlou

    Article

    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

    Insert

    NLS-NG-T2A-hSHH
    

    Use

    Piggybac

    Tags

    NLS-NG-T2A

    Expression

    Mutation

    Promoter

    CAGp

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV-BE4-PpAPOBEC1

    Plasmid

    #138349

    Purpose

    Mammalian expression plasmid for BE4-PpABOBEC1

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-PpAPOBEC1
    

    Use

    Tags

    BP-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAG-OSF-KATNA1

    Plasmid

    #160051

    Purpose

    Mammalian expression vector for KATNA1 (KATANIN-P60) with N-terminal One-Strep-Flag tag. Internal ID: WISP20-18.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Tags

    One-Strep-Flag (OSF)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGLACTE-PseudoHT51

    Plasmid

    #99257

    Purpose

    Contains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresis

    Depositor

    Karen Usdin

    Article

    Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi

    Insert

    FMR1 (FMR1 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mutation

    Contains synthetic, non-human, primer sequence do…

    Promoter

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

    Plasmid

    #101139

    Purpose

    Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDONR221_SLC25A26

    Plasmid

    #132259

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A26 (SLC25A26 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. Flag‐mTim3

    Plasmid

    #49208

    Purpose

    expresses Flag tagged mouse Tim3

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 (Havcr2 Mouse)
    

    Use

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    no Tim3 signal sequence

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-BE4-AmAPOBEC1

    Plasmid

    #138342

    Purpose

    Mammalian expression plasmid for BE4-AmABOBEC1

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-AmAPOBEC1
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TFORF0209

    Plasmid

    #141556

    Purpose

    Lentiviral vector for overexpressing the FOXP2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXP2 (FOXP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pER8 cHA-FG1

    Plasmid

    #118689

    Purpose

    Plant inducible expression

    Depositor

    Kristiina Himanen

    Article

    Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

    Insert

    AT3G07200 (AT3G07200 Mustard Weed)
    

    Use

    Tags

    HA

    Expression

    Plant

    Mutation

    Promoter

    LexA

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBiT3.1-N_RACK1:1-317

    Plasmid

    #211110

    Purpose

    Mammalian expression of full-length human RACK1. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    RACK1:M1-R317 (RACK1 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge