167,948 results

1. pDisplay-FRISZ

   Plasmid

   #176887

   Purpose

   Far-red fluorescent zinc indicator FRISZ in pDisplay

   Depositor

   Huiwang Ai

   Article

   Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

   Insert

   FRISZ
   

   Tags

   pDisplay backbone elements for export and membran…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139087

   Purpose

   Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   SpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMP4639

   Plasmid

   #107771

   Purpose

   Expression of EYFP

   Depositor

   Herman Spaink

   Article

   Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   pLacZ-EYFP in pME6010
   

   Tags

   pLacZ-EYFP

   Expression

   Bacterial

   Promoter

   pLacZ

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC-TALO8

   Plasmid

   #83409

   Purpose

   TRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeast

   Depositor

   Toshi Tsukiyama

   Article

   Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

   Insert

   TRP-ARS-8xLacO
   

   Expression

   Yeast

   Mutation

   Please see depositor comments below

   Promoter

   TRP1

   Available Since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSMVP-Cas9FL-P2A-turboGFP

   Plasmid

   #80941

   Purpose

   Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9FL
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. ptwB

   Plasmid

   #48735

   Purpose

   Tandem dual tomato plasmid

   Depositor

   Roger Tsien, John Schiller

   Article

   Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

   Insert

   Tandem dual tomato
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. 5VBD (USP09X)

   Plasmid

   #101260

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   USP09X (USP9X Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 2, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pVITRO-HPV38 L1L2

   Plasmid

   #52594

   Purpose

   Expresses HPV38 L1 and L2 in mammalian cells

   Depositor

   Richard Roden

   Article

   Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

   Inserts

   HPV38 L1
   HPV38 L2
   

   Expression

   Mammalian

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. GreenGate

   Plasmid Kit

   #1000000036

   Purpose

   Golden Gate cloning system for rapid assembly of plant transformation constructs; Uses 6 types of pre-cloned modules: promoter, N- and C-terminal tags, coding sequence, terminator, resistance cassette

   Depositor

   Jan Lohmann

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pNES-EGFP-C1-flex-PABD-Spo20

    Plasmid

    #220081

    Purpose

    Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    Spo20 (SPO20 Budding Yeast)
    

    Tags

    X. leavis map2k1.L(32-44)-EGFP-SGGGSGGS

    Expression

    Mammalian

    Mutation

    amino acids 51-91

    Promoter

    CMV

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV V5-LAP2beta

    Plasmid

    #175112

    Purpose

    Lentiviral expression of V5-tagged mouse Tmpo

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmpo (Tmpo Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-HA-FbxO11-ΔFbox-pcDNA3.1-

    Plasmid

    #52502

    Purpose

    expresses human FbxO11-ΔFbox in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    deletion of Fbox (deleted aa 71-116)

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. DL034:cFos::HaloTag-iPAK4

    Plasmid

    #177884

    Purpose

    HaloTag-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    cFos

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-7 asyn S129A

    Plasmid

    #36048

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

    Insert

    alpha-synuclein S129A (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    S129A

    Promoter

    T7

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS MCK CRE

    Plasmid

    #12529

    Depositor

    Ronald Kahn

    Article

    Bruning et al Mol Cell. 1998 Nov . 2(5):559-69.

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Transgenic construct

    Mutation

    Cre is driven by the Muscle Creatine Kinase promo…

    Available Since

    Aug. 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221_SLC2A12

    Plasmid

    #132067

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC2A12 (SLC2A12 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. PRRX2-pSG5

    Plasmid

    #21009

    Depositor

    Corey Largman

    Article

    Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

    Insert

    PRRX2 (Prrx2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1-Puro CAG-CLTX CD32a-tm CD3z CAR

    Plasmid

    #171963

    Purpose

    Donor plasmid for CLTX T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

    Insert

    Synthetic CLTX CD32a-tm CD3z CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLuc-3'UTR

    Plasmid

    #42094

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    ZEB1 3UTR (ZEB1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CBA-DIO-GCaMP6s-P2A-mBeRFP-WPRE-pA

    Plasmid

    #175177

    Purpose

    Cre-dependent GCaMP6s and mBeRFP expression

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-mBeRFP

    Promoter

    CAG

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLV sgRNA-Notch1 #1 GFP

    Plasmid

    #106950

    Purpose

    Lentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    gRNA targeting Notch1 (Notch1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. GFP-Dcp1a

    Plasmid

    #153972

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1a

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA decapping enzyme 1a (DCP1A Human)
    

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pC1-IMS-oROS-HT

    Plasmid

    #216419

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the inter-membrane-space of mitochondria.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pMpGWB123

    Plasmid

    #68577

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Triple repeats of Citrine (3xCitrine)

    Expression

    Plant

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAL1

    Plasmid

    #197285

    Purpose

    Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195

    Depositor

    Bogumil Karas

    Article

    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

    Insert

    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Available Since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET SUMO NL-FKBP-Cas9

    Plasmid

    #162730

    Purpose

    Bacterial expression, protein expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. GST-Rab8a-QL

    Plasmid

    #86079

    Purpose

    A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed glutamine 67 to leucine

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPOTv7-g418-3mNG

    Plasmid

    #179832

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Keith Gull, Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    3x mNG
    

    Use

    Unspecified

    Promoter

    read through

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA5/FRT/TO HIS HSPA6

    Plasmid

    #19540

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 6 (HSPA6 Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. gRNA-HEK3-Puro

    Plasmid

    #136282

    Purpose

    Expresses a guide RNA targeting HEK3 site

    Depositor

    Zhaohui Ye

    Article

    McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

    Insert

    sgRNA-HEK3
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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31. pDONR223_RIMS2_WT

    Plasmid

    #81803

    Purpose

    Gateway Donor vector containing RIMS2 ,part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RIMS2 (RIMS2 Human)
    

    Use

    Gateway entry vector

    Mutation

    S86F; Q295R

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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32. pCK007 pME KalTa4

    Plasmid

    #195965

    Purpose

    Gateway pME Gal4 variant

    Depositor

    Christian Mosimann, Alexa Burger

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    KalTA4 (Gal4)
    

    Use

    Other

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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33. pGBW-m4133611

    Plasmid

    #152146

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. GST-Rab8a-TN

    Plasmid

    #86080

    Purpose

    A bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed threonine 22 to aspargine

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSN-A112C-GpA-G83I

    Plasmid

    #191239

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    

    Tags

    nuclease A from S. aureus fused to the TM domain …

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and Glycin…

    Promoter

    T7 promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSN-A112C-GpA

    Plasmid

    #191238

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    

    Tags

    nuclease A from S. aureus fused to the TM domain …

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine

    Promoter

    T7 promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. KA2897_pPB-TREtight-UBCmin-cHA-IRESH2BmKO2

    Plasmid

    #124176

    Purpose

    piggyBac-based Dox-inducible expression (moderate)

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pOGG202

    Plasmid

    #115503

    Purpose

    Clone of a level 1 unit which encodes IPTG-inducible sfGFP

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    pL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pOVC1_ScPH1-S_X

    Plasmid

    #131184

    Purpose

    Expresses ScPH1-S (Synechosystis PCC6803) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    ScPH1-S
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LRG/Foxa1 e2.4

    Plasmid

    #105511

    Purpose

    Lentiviral expression of Foxa1 DNA-binding domain (exon 2) targeting sgRNA

    Depositor

    Christopher Vakoc

    Article

    Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

    Insert

    Foxa1 (sgRNA, e2.4)
    

    Use

    Lentiviral

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge