Addgene

We narrowed to 1,954 results for: Endog

Showing: 1301 - 1320 of 1954 results

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pAPInt-mScarlet-Nat

Plasmid

#236491

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

mScarlet

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mCherry-Hyg

Plasmid

#236489

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

mCherry

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-GFP-Nat

Plasmid

#236476

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-Dendra2-Hyg

Plasmid

#236495

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

Dendra2

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mNeonGreen-Hyg

Plasmid

#236483

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mScarlet-Hyg

Plasmid

#236492

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

mScarlet

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-GFP-Hyg

Plasmid

#236477

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-sfGFP-Nat

Plasmid

#236479

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

sfGFP

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mNeonGreen-Nat

Plasmid

#236482

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Vamp2-TagIN-HA

Plasmid

#196494

Purpose

To tag endogenous mouse VAMP2 with HA

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Inserts

HA
gRNA

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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KBB11

Plasmid

#185422

Purpose

Full-length KAP60/SRP1

Depositor

Laura Davis, Ken Belanger

Article

Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

Insert

KAP60

Use

Tags

Expression

Yeast

Mutation

Promoter

endogenous

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRS315Nab3Q->E

Plasmid

#110538

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

O'Rourke et al PLoS One. 2016 Mar 8;11(3):e0150865. doi

Insert

NAB3 (NAB3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Q to E mutations in Nab3 low complexity domain

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pJK500

Plasmid

#72245

Purpose

Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

Depositor

T. J. Kappock

Inserts

N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase

Use

Tags

Expression

Bacterial

Mutation

Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

Promoter

T7 and endogenous

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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EasyFusion mAID-mCherry with Neo

Plasmid

#113831

Purpose

EasyFusion vector for targeting endogeneous genes with mAID-mCherry

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

mAID-mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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CelTag Plasmid

Plasmid

#66562

Purpose

Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

Depositor

David Engelke

Article

Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

Insert

CelTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCM-HA-Gas1

Plasmid

#38313

Depositor

Susan Lindquist

Article

McLellan et al ACS Chem Biol. 2012 Jun 22.

Insert

Gas1 (GAS1 Budding Yeast)

Use

Tags

2xHA

Expression

Mutation

contains promoter region

Promoter

Endogenous

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-LEXY (pDN157)

Plasmid

#72658

Purpose

Constitutively nuclear LEXY variant for blocking endogenous, CRM-1-dependent nuclear export with blue light

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

H2B-GFP-LEXY

Use

Tags

Expression

Mutation

Promoter

CMV

Available since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMT-halo-HA-p2a-neoR

Plasmid

#161977

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GAlinker-HA-halo-HA-p2a-neoR

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-puroR-p2a-HA-halo

Plasmid

#161979

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

puroR-p2a-HA-halo-GSlinker

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAPInt-mNeonGreen-G418

Plasmid

#236484

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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