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pC4M-F2E-GFP-FKBP

Plasmid

#68058

Purpose

express EGFP-tagged FKBP for heterodimerization with FRB in mammalian cells

Depositor

Richard Youle

Article

Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

Insert

EGFP

Tags

2xFKBP and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pSP64-mHCN2

Plasmid

#53060

Purpose

Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Chen et al J Biol Chem. 2000 Nov 17;275(46):36465-7

Insert

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2 Mouse)

Use

Xenopus oocyte expression

Mutation

E55G, R237H, and K283R polymorphisms compared to …

Promoter

SP6

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pDsRed-miR9 Sensor

Plasmid

#22742

Depositor

Lynn Hudson

Article

Lau et al J Neurosci. 2008 Nov 5. 28(45):11720-30.

Insert

microRNA 9 binding sites

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP

Plasmid

#156448

Purpose

For transient expression of human CTCF tagged with eGFP

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

human CTCF (CTCF Human)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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BPK2014-AaCas12b

Plasmid

#121949

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b

Tags

10xHis tag

Expression

Bacterial

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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AMPK-γ1-HA

Plasmid

#40605

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-γ1 (Prkag1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pSP64-hKCNQ1

Plasmid

#53048

Purpose

Expresses alpha subunit for hKCNQ1 channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

Insert

Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)

Use

Xenopus oocyte expression

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab2WT

Plasmid

#46779

Purpose

expression of WT Rab2a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB2 (RAB2A Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pML433

Plasmid

#177190

Purpose

Expresses recombinant HyPro enzyme in E. coli

Depositor

Eugene Makeyev

Article

Yap et al Mol Cell. 2021 Nov 2. pii: S1097-2765(21

Insert

HyPro enzyme

Expression

Bacterial

Promoter

T7

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTAL7b

Plasmid

#48706

Purpose

TALEN expression in mammalian cells

Depositor

Boris Greber

Article

Frank et al BMC Genomics. 2013 Nov 9;14(1):773.

Type

Empty backbone

Use

TALEN

Tags

FokI nuclease

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pGL4 mIL-17 0.6kb promoter

Plasmid

#20126

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only
pSC101-pT7-T3RNAP

Plasmid

#140872

Purpose

T7 promoter driven synthesis of T3 RNA polymerase

Depositor

Neal Devaraj

Article

Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

Insert

T3 RNA polymerase

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-FLAG-CHK1

Plasmid

#86875

Purpose

To express CHK1 with a FLAG tag at N-terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

CHEK1 (CHEK1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-GECO2

Plasmid

#159603

Purpose

AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2

Depositor

Robert Campbell

Article

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Insert

NIR-GECO2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pmRFP-N1 human cofilin S3E

Plasmid

#50858

Purpose

expresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available Since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
pML1342

Plasmid

#32376

Depositor

Michael Niederweis

Article

Huff et al Gene. 2010 Nov 15;468(1-2):8-19. Epub 20

Insert

int L5; xylEm; ttsbiA, ttsbiB

Use

Bacterial integration vector

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pKO2.2

Plasmid

#22676

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

DTA

Use

Mouse Targeting

Mutation

pDTA for targeting vector generation. I-CeuI lin…

Available Since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-CD4-FLAG

Plasmid

#51604

Purpose

expresses CD4 with C-terminal FLAG tag

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

CD4 (CD4 Human)

Tags

FLAG and myc

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pC4-RhE-FRB-Fis1

Plasmid

#68056

Purpose

express Fis1 tail-anchored FRB for heterodimerization with FKBP in mammalian cells

Depositor

Richard Youle

Article

Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

Insert

Fis1 (FIS1 Human)

Tags

FRB

Expression

Mammalian

Mutation

Fis1 tail (aa 93-152)

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLTR-RVG

Plasmid

#17577

Depositor

Jakob Reiser

Article

Mochizuki et al J Virol. 1998 Nov . 72(11):8873-83.

Insert

rabies virus glycoprotein (SAD B19 strain)

Use

Lentiviral

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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