We narrowed to 2,571 results for: piggyBac
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Plasmid#133369PurposePiggybac vector for constitutive expressionDepositorInsertPLAGL2 (PLAGL2 Human)
TagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAT-PB-CG2APtk
Plasmid#86003Purposehomology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassetteDepositorInserteGFP_T2A_puro_dTK
UsePiggybac exciseableExpressionMammalianPromoterCAGAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-T3G-CERP2
Plasmid#175498PurposeAll-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianPromoterTRE3GAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2-MG
Plasmid#175501PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianPromoterTRE3G-mCMVAvailable sinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAS_4xMma PylT(UUA)_FLAG-AcKRS
Plasmid#140010PurposePlasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) suppression; for transient or stable piggyBac-mediated integrationDepositorInsertAcKRS-3 (pylS Methanosarcina mazei)
TagsFLAGExpressionMammalianMutationL301M, L305I, Y306F, L309A, C348FPromoterEF1Available sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT_FLAG-Mx PylRS YA
Plasmid#140012PurposePlasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianMutationY126APromoterEF1Available sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
KA1042_pPB-hCMV*1-Esrrb-IV
Plasmid#124177PurposepiggyBac-based Dox-inducible expression of EsrrbDepositorAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OKMS
Plasmid#80480PurposepiggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)DepositorUsePiggybac transposonExpressionMammalianMutationKlf4 [10-483]PromotertetOAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG
Plasmid#140018PurposePlasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertEcoTyrRS
TagsFLAGExpressionMammalianMutationY37L, D182S, F183M, L186APromoterEF1Available sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-Hyg-Dest-mCherry-PAR-6B
Plasmid#194908PurposeTo generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.DepositorInsertPAR-6B (Pard6b Mouse)
UseMouse TargetingTagsmCherryExpressionMammalianMutation39 C to G, silent mutationAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2
Plasmid#175497PurposeAll-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianPromoterTRE3G-mCMVAvailable sinceOct. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR
Plasmid#170808PurposePiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistanceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsExpressionMammalianMutationSynonymous mutation in PYL1 to remove XhoI site (…Available sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_FLAG-Mx1201 PylRS
Plasmid#154764Purposeplasmid with Mx1201 PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertMx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianPromoterEF1Available sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
KA2897_pPB-TREtight-UBCmin-cHA-IRESH2BmKO2
Plasmid#124176PurposepiggyBac-based Dox-inducible expression (moderate)DepositorTypeEmpty backboneExpressionMammalianAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1065_pPB-hCMV*1-cHA-IRESH2BmCherry
Plasmid#124170PurposepiggyBac-based Dox-inducible expressionDepositorTypeEmpty backboneExpressionMammalianAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1130_pPB-TREtight-cHA-IRESH2BmCherry
Plasmid#124173PurposepiggyBac-based Dox-inducible expressionDepositorTypeEmpty backboneExpressionMammalianAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-eDIO-ChR2-EYFP
Plasmid#104387PurposePiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertChannelrhodopsin 2
UseCre/Lox; PiggybacTagsEYFPExpressionMammalianMutationE134RAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg83-PDGFR_EF1a_iRFP670
Plasmid#186311PurposePlasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg83-PDGFR
iRFP670
TagsHis, mycExpressionMammalianAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg88-PDGFR_EF1a_eBFP2
Plasmid#186312PurposePlasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.DepositorInsertssg88-PDGFR
eBFP2
TagsHis, mycExpressionMammalianAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA
Plasmid#140014PurposePlasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianMutationY126A, Y197FPromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only