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Plasmid#102929PurposeExpress a lysosomal TagDepositorInsertTmem192 (TMEM192 Human)
UseLentiviralTagsFlagExpressionMutationPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX-sgRNA
Plasmid#50662PurposeLentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)DepositorInsertsgAAVS1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
N1-Tq-Ca-FLITS
Plasmid#191454PurposeGenetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changesDepositorInsertTq-Ca-FLITS
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
PVS10
Plasmid#104398Purposepolycistronic vector for expression of E. coli core RNAPDepositorUseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-ATG8(416)/GFP-AUT7(416)
Plasmid#49425PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.DepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseTagsGFPExpressionBacterial and YeastMutationPromoterATG8Available sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hSyn-DIO-EGFP (AAV8)
Viral Prep#50457-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBinit
Plasmid#110100PurposeE.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTagDepositorTypeEmpty backboneUseTagsmyc, HisExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTP955
Plasmid#104164PurposeBacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)DepositorInsertAnti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMALB-ATF4.5
Plasmid#155032PurposeFluorescent reporter for ATF4 translationDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag
Plasmid#132402Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059APromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-mSTING
Plasmid#214157PurposeTransient expression of STINGDepositorInsertSTING (Sting1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-NfiB
Plasmid#64900PurposeInducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.DepositorInsertNfiB (Nfib Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTetOAvailable sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-L-R279E
Plasmid#182286PurposeMammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDXinit
Plasmid#110101PurposeE.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phagesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flpo (AAV Retrograde)
Viral Prep#55637-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-EF1a-Flpo (#55637). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flpo plasmid DNA. EF1a-driven Flpo recombinase expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K48
Plasmid#17605PurposeMammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK48 only, other lysines mutated to arginines. Enh…PromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
hCRISPRi_dual_1_2
Pooled Library#187246PurposeUltra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.DepositorSpeciesSyntheticUseLentiviralAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-PsppA-mCherry
Plasmid#225428PurposePlasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherryDepositorInsertmCherry, Cas9, SgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterPsppA upstream of mCherry and Cas9, P3 upstream o…Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
ymScarlet
Plasmid#111917Purposeyeast optimized mScarletDepositorInsertymScarlet
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-MITF-A
Plasmid#38132DepositorInsertMITF-A (MITF Human)
UseTagsEGFPExpressionMammalianMutationdeleted stop codonPromoterCMVAvailable sinceJuly 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-MITF-M
Plasmid#38131DepositorInsertMITF-M (MITF Human)
UseTagsEGFPExpressionMammalianMutationdeleted stop codonPromoterCMVAvailable sinceJuly 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV-Brainbow-2.1 R
Plasmid#18723DepositorInserthrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP
Plasmid#158962PurposeCre-dependent Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed DsRed cassette
Cas9-GFP
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry
Plasmid#85430Purpose[Edit] Lentiviral vector for Gal4 inducible human IL-10/PD-L1 with constitutive mCherry expressionDepositorInserthuman IL-10 T2A PD-L1
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR
Plasmid#191795Purposevector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6DepositorInserthumanized hfCas13d
UseAAV and CRISPRTagsExpressionMammalianMutationA134V, A140V, A141V, A143VPromoterEFS, hU6Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
R-pre-mRFP
Plasmid#17275DepositorInsertR-pre
UseTagsmRFPExpressionMammalianMutationPromoterAvailable sinceApril 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
GFP-CYLD-STOP
Plasmid#60077PurposeN-terminal GFP tagged CYLD with stop codon, driven by CMV promoterDepositorInsertCylindromatosis (CYLD Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-EBFP2-NLS (pRW1447)
Plasmid#216219PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1.tR4-MbPyl
Plasmid#50832Purposebaculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cellsDepositorInsertstwo-copy Pyl tRNA cassette
MbPyl
two copy Pyl tRNA cassette
UseBaculoviralTagsExpressionInsectMutationPromoterCMV and U6 and H1Available sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only