165,637 results

1. LentiCRISPR v2 hBAK

   Plasmid

   #129579

   Purpose

   CRISPR deletion of human BAK

   Depositor

   Stephen Tait

   Article

   Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

   Insert

   gRNA vs human BAK (BAK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPB-cT3G-cERP2-GATA4

   Plasmid

   #192896

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of GATA4

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   GATA4 (GATA4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Codon-optimized

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAcCas12nHS_RV6_empty

   Plasmid

   #203811

   Purpose

   Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12nHS_RV6
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RN3P-KDM5B-FLAG

   Plasmid

   #86398

   Purpose

   FLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression

   Depositor

   Wei Xie

   Article

   Zhang et al Nature. 2016 Sep 14;537(7621):553-557. d

   Insert

   KDM5B (Kdm5b Mouse)
   

   Use

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Lac

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHR_TRE_Ncad-P2A-GFPlig

   Plasmid

   #133806

   Purpose

   inducible expression of Ncad and membrane-tethered GFP in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2018 Jul 13;361(6398):156-162.

   Insert

   cadherin 2 (CDH2) (Cdh2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLPO1

   Plasmid

   #209975

   Purpose

   Contains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   replication origin (256_rep)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. CLIMP-63 pcDNA

   Plasmid

   #80977

   Purpose

   mammalian expression of CLIMP-63

   Depositor

   Peter Steyger

   Article

   Karasawa et al Cell Death Dis. 2010 Nov 18;1:e102. doi:

   Insert

   CLIMP-63 (CKAP4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-DIO-BACE-HA

   Plasmid

   #232353

   Purpose

   Beta-Secretase to be expressed (1:1) with either ATLAS protein.

   Depositor

   Don Arnold

   Article

   Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

   Insert

   BACE (BACE1 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CAG-GFP (AAV8 trial size)

   Viral Prep

   #37825-AAV8.T

   Purpose

   Ready-to-use AAV8 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   GFP

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-Ef1a-DIO EYFP (AAV9)

    Viral Prep

    #27056-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, cre-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Tags

    EYFP (Cre-dependent)

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

11. myc-KIF5B

    Plasmid

    #127617

    Purpose

    N-terminal myc-tagged mouse KIF5B

    Depositor

    Josef Kittler

    Article

    Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

    Insert

    KIF5B (Kif5b Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Human LFA-1 alpha

    Plasmid

    #8630

    Depositor

    Timothy Springer

    Article

    Larson et al J Cell Biol 1989 Feb;108(2):703-12.

    Insert

    LFA1 alpha (ITGAL Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

13. AAV phSyn1(S)-tdTomato-WPRE

    Plasmid

    #51506

    Purpose

    Can be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Has Service

    AAV5

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    phSyn1

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMyNT-kan

    Plasmid

    #84693

    Purpose

    Protein expression in mycobacteria

    Depositor

    Matthias Wilmanns

    Article

    Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Acetamidase

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mTOR_2 shRNA

    Plasmid

    #1856

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    mTOR (MTOR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TetOn-REEP5-mCherry-eGFP

    Plasmid

    #139870

    Purpose

    Dox-inducible lentiviral expression of human REEP5

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    REEP5 (REEP5 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-eGFP

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDusk

    Plasmid

    #43795

    Purpose

    Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

    Depositor

    Andreas Moeglich

    Article

    Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSB819-PKR-hum

    Plasmid

    #20030

    Depositor

    Harmit Malik

    Article

    Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

    Insert

    Protein kinase R (EIF2AK2 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK-Myc-Smurf1

    Plasmid

    #13676

    Depositor

    Ying Zhang

    Article

    Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

    Insert

    Smurf1 (SMURF1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTOR_1 shRNA

    Plasmid

    #1855

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    mTOR (MTOR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. nLuc-GLP-1R-His-FLAG

    Plasmid

    #124831

    Purpose

    Expresses N-terminally NanoLuc-Tagged GLP-1R in mammalian cells

    Depositor

    Sam Gellman

    Article

    Cary et al Nat Chem Biol. 2022 Mar;18(3):256-263. d

    Insert

    GLP1R (GLP1R Human, Synthetic)
    

    Use

    Luciferase

    Tags

    FLAG, His6, and NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV CMV mCherry-DDX1-V5

    Plasmid

    #175162

    Purpose

    Lentiviral expression of human DDX1-V5

    Depositor

    Larry Gerace

    Article

    Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

    Insert

    DDX1 (DDX1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-hSyn-Cre-P2A-dTomato (AAV1)

    Viral Prep

    #107738-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Rylan Larsen

    Promoter

    Syn

    Tags

    dTomato (physically separate, not a fusion protein)

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

24. pBMN BFP-Parkin

    Plasmid

    #186221

    Purpose

    For expression of Parkin tagged with BFP

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP-N1-ACTR3

    Plasmid

    #8462

    Depositor

    Matthew Welch

    Article

    Welch et al J Cell Biol 1997 Jul 28;138(2):375-84.

    Insert

    ACTR3 (ACTR3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSIQmate-EGFP

    Plasmid

    #216241

    Purpose

    All-in One IQ-Cumate transgene switch for the EGFP expression

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCS2-Jmjd3-F

    Plasmid

    #17440

    Purpose

    Expression of Jmjd3 in mammalian cells

    Depositor

    Kai Ge

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

    Insert

    JMJD3 (Kdm6b Mouse)
    

    Use

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBK- Ma PylRS WT

    Plasmid

    #197573

    Purpose

    Expresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Insert

    M. alvus PylRS WT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    GlnS (constitutive)

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS

    Plasmid

    #154774

    Purpose

    plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    in PylT: UUA anticodon and G10A, G14U, G16U, C20U…

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPID2

    Plasmid

    #169898

    Purpose

    Minimal empty vector with piggyBac, insulators, and a DTS

    Depositor

    Richard Schneider

    Article

    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge