We narrowed to 5,105 results for: Braf

1. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

   Plasmid

   #75154

   Purpose

   Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   H2B-mCerulean3-P2A-mKOFP2-CAAX
   

   Use

   Middle entry vector for multisite gateway three f…

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTone-gata2aECE-Kaede

   Plasmid

   #132976

   Purpose

   gata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backbone

   Depositor

   Nathan Lawson

   Article

   Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

   Inserts

   Kaede
   gata2a endothelial enhancer (x6) and a carp b-actin basal promoter
   

   Use

   Unspecified; Transposon-mediated

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSlick-Neo-RECK

   Plasmid

   #121986

   Purpose

   Tetracycline-inducible expression of untagged full-length RECK

   Depositor

   GuangJun Zhang

   Article

   Kumari et al Oncotarget. 2018 May 4;9(34):23494-23504

   Insert

   RECK (RECK Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Tight TRE promoter

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2TAL3-RR

   Plasmid

   #37276

   Purpose

   TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

   Depositor

   David Grunwald

   Article

   Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

   Type

   Empty backbone

   Use

   Tags

   C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF-ABEmax

   Plasmid

   #164415

   Purpose

   EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   ABEmax
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+ mWnt5a

   Plasmid

   #102860

   Purpose

   Expresses mouse Wnt5a in mammalian cells, zebrafish or Xenopus

   Depositor

   Henry Ho

   Article

   Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

   Insert

   Mouse Wnt5a
   

   Use

   Zebrafish expression; xenopus expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV; SP6

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

   Plasmid

   #191500

   Purpose

   GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye

   Depositor

   Michael Granato

   Article

   Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

   Insert

   CaSR-EGFP-SV40
   

   Use

   Creation of transgenic fish using tol2 transgenes…

   Tags

   Expression

   Mutation

   Promoter

   myo6b

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AsCas12a-2C-NLS in pCSDest

   Plasmid

   #126637

   Purpose

   Expresses AsCas12a-2C-NLS in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   AsCas12a-2C-NLS
   

   Use

   CRISPR

   Tags

   3xHuman influenza hemagglutinin (HA)-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94 promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p13xQUAS-MCS, α-cry:mCherry

   Plasmid

   #180481

   Purpose

   Inducible gene expression vector p13xQUAS-MCS, α-cry:mCherry

   Depositor

   Hyunju Ro

   Article

   Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

   Insert

   Multiple cloning site
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG657 H2B-pFAST

    Plasmid

    #172862

    Purpose

    Expresses pFAST fused to zebrafish histone H2B in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    H2B-pFAST
    

    Use

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ZIKV_Sensor_32B_LacZ

    Plasmid

    #75007

    Purpose

    Toehold switch sensor to detect Zika Virus

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_sensor_32B_LacZ
    

    Use

    Tags

    LacZ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Tol2-LyzC-GFP-U6ac-ctrl-guides

    Plasmid

    #168240

    Purpose

    "neutrophil specific GFP with ubiquitous ctrl sgRNAs"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    control sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ZIKV_Trigger_27B

    Plasmid

    #75008

    Purpose

    Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequence

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_Trigger_27B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTol2-elavl3-WHaloCaMP1a

    Plasmid

    #205312

    Purpose

    Expression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FnoCas12a-2C-NLS in pCSDest

    Plasmid

    #126639

    Purpose

    Expresses FnoCas12a-2C-NLS in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnoCas12a-2C-NLS
    

    Use

    CRISPR

    Tags

    3xHuman influenza hemagglutinin (HA)-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94 promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT2-cryR;dll4P1Egfp

    Plasmid

    #90160

    Purpose

    contains dll4 specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    dll4P1Egfp (dll4 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ZIKV_Trigger_32B

    Plasmid

    #75009

    Purpose

    Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_Trigger_32B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2-ER-EmEGFP

    Plasmid

    #169000

    Purpose

    Express ER-EmEGFP in zebrafish

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Synthetic, Human)
    

    Use

    Unspecified

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT2-cryR;lmo2P1Egfp

    Plasmid

    #90162

    Purpose

    contains lmo2 specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    lmo2P1Egfp (lmo2 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Tol2-LyzC-GFP-U6ac-rac2-guides

    Plasmid

    #168241

    Purpose

    "neutrophil specific GFP with ubiquitous rac2 sgRNAs"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    rac2 sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCK072 p5E 147bp exorh:EGFP

    Plasmid

    #195950

    Purpose

    Gateway p5E vector with reporter

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    exorh minimal regulatory elements and EGFP
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 782 pMEActMEK

    Plasmid

    #31166

    Depositor

    Nathan Lawson

    Article

    Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

    Insert

    MEK (MAP2K1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Ser218Glu Ser222Asp; activated MEKK

    Promoter

    Available since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

23. 1352wUV2 F2bbs kan GCANNNAGGk

    Plasmid

    #18752

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    3 tandem zinc fingers
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A Kan cassette (1090bp) is inserted between two b…

    Promoter

    Available since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid

    #75177

    Purpose

    Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    H2B-mCerulean3-P2A-mKOFP2-CAAX
    

    Use

    Three prime entry vector for multisite gateway th…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Tol2-LyzC-GFP-U6ac-cdk2-guides

    Plasmid

    #168250

    Purpose

    "neutrophil specific GFP with ubiquitous cdk2 sgRNAs"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    cdk2 sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 1352wUV2 F3bbs kan NNNTGGGCGk

    Plasmid

    #18756

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    3 tandem zinc fingers
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A Kan cassette (1090bp) is inserted between two b…

    Promoter

    Available since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 1352wUV2 F1bbs kan GCGTGGNNN

    Plasmid

    #18753

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    3 tandem zinc fingers
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A Kan cassette (1090bp) is inserted between two b…

    Promoter

    Available since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ath5:H2B-RFP

    Plasmid

    #105960

    Purpose

    transgenesis, appears rather late, likely due to long RFP maturation time but is brighter than most other Ath5 construct

    Depositor

    Caren Norden

    Article

    Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

    Insert

    H2B (H2BC21 Human)
    

    Use

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR221-kcnj11

    Plasmid

    #173139

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj11 (kcnj11 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. sox10:PDGFRA wild-type

    Plasmid

    #98696

    Purpose

    I-SceI meganuclease-mediated human PDGFRA wild-type transgenesis in zebrafish

    Depositor

    Thomas Look

    Article

    Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

    Insert

    PDGFRA (PDGFRA Human)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. bactin:mKate2-ras

    Plasmid

    #105963

    Purpose

    transgenesis, ubiquitous expression

    Depositor

    Caren Norden

    Article

    Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

    Insert

    Ras (KRAS Human)
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 360 pCR kdrb

    Plasmid

    #13067

    Depositor

    Nathan Lawson

    Article

    Covassin et al Proc Natl Acad Sci U S A. 2006 Apr 25. 1

    Insert

    kdrb (kdr Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 18, 2010

    Availability

    Academic Institutions and Nonprofits only

33. sox10:PDGFRA mutant

    Plasmid

    #98697

    Purpose

    I-SceI meganuclease-mediated human PDGFRA mutant transgenesis in zebrafish

    Depositor

    Thomas Look

    Article

    Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

    Insert

    PDGFRA (PDGFRA Human)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    exon 8 and 9 deletion

    Promoter

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pZE21-APX(W41F) dimeric

    Plasmid

    #58199

    Purpose

    Bacterial expression of APX W41F under control of TetR

    Depositor

    Alice Ting

    Article

    Dwyer et al Proc Natl Acad Sci U S A. 2014 May 20;11

    Insert

    pea APX W41F
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    W41F

    Promoter

    pL(tetO-1)

    Available since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTRC-APX(W41F) dimeric

    Plasmid

    #58499

    Purpose

    Bacterial expression of APX W41F inducible by IPTG

    Depositor

    Alice Ting

    Article

    Dwyer et al Proc Natl Acad Sci U S A. 2014 May 20;11

    Insert

    pea APX W41F (dimeric)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    W41F

    Promoter

    trc

    Available since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Nanog-mNG-KS-FtoG

    Plasmid

    #238297

    Purpose

    For RNA injection of Nanog-mNG-KS-FtoG into Zebrafish

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    Nanog-mNG-KS-FtoG
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAX1_Z11 gpN:SpyTag

    Plasmid

    #225196

    Purpose

    Used for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SpyTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAX1_HS∆tum

    Plasmid

    #225198

    Purpose

    Used to create a markerless deletion of the E. coli HS tum gene

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    tum homology arms
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAX1_HS_lexA(Ind-)

    Plasmid

    #225199

    Purpose

    Used to create a non-inducible lexA by introducing a G85D mutation in E. coli HS

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    lexA homology arms
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    lexA G85D

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAX1_HS_gpN:SpyTag

    Plasmid

    #225193

    Purpose

    Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SpyTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge