We narrowed to 11,866 results for: KIN

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pET28a_Pro-IL-33

Plasmid

#214342

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL33 (IL33 Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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HHV6 U69

Plasmid

#26689

Depositor

Robert Kalejta

Article

Kuny et al PLoS Pathog. 2010 . 6(9):.

Insert

U69 (U69 Human Herpes Virus 6)

Tags

HA

Expression

Mammalian

Available Since

Nov. 1, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PAK7

Plasmid

#23850

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK7 (PAK5 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CKM

Plasmid

#23866

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CKM (CKM Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PCTK3

Plasmid

#23710

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PCTK3 (CDK18 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pET28_human-Caspase-4_enzymatic-domain

Plasmid

#183381

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-4 (AA94-377) (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM1-Flag-Rheb

Plasmid

#165031

Purpose

expression of Flag tagged Rheb protein in mammalian cells

Depositor

Ken Inoki

Article

Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

Insert

Rheb (RHEB Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-ALMS1

Plasmid

#136828

Purpose

Mammalian expression of the centrosomal protein ALMS1 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-ALMS1 (ALMS1 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgAMPKa1

Plasmid

#138704

Purpose

Expresses a human AMPKa1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgAMPKa1 human (PRKAA1 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-TRAF6-C70A

Plasmid

#43930

Depositor

Bryant Darnay

Article

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Insert

TNF Receptor-Associated Factor 6 (Traf6 )

Tags

GST

Expression

Bacterial

Mutation

C70A

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES-hSphK2-EGFP

Plasmid

#84371

Purpose

Expression of exogenous human SphK2 in mammalian cells

Depositor

Anthony Don, Hugh Rosen

Article

Don et al J Biol Chem. 2007 May 25;282(21):15833-4

Insert

SPHK2 (SPHK2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only
pGZ110 (pFA6a-3FLAG-MNase-TRP1)

Plasmid

#70233

Purpose

ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope tag

Depositor

Steven Henikoff

Article

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

3xFLAG tag and aa 83-231 of Mnase

Use

Yeast genomic targeting

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-AP1S3

Plasmid

#58292

Purpose

mammalian expression of AP1S3

Depositor

Francesca Capon

Article

Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

Insert

AP1S3 (AP1S3 Human)

Tags

6xHis and myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
MSCV-myrAkt

Plasmid

#49267

Purpose

retroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequence

Depositor

Lawrence Kane, Arthur Weiss

Article

Kane et al Nat Immunol. 2001 Jan;2(1):37-44.

Insert

Akt (AKT1 Bovine)

Use

Retroviral

Tags

IRES-EGFP and myr

Expression

Mammalian

Mutation

N2D, D149E and A478G compared to AAW71957.1

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only
pET28a-Beclin1 (1-242)

Plasmid

#24401

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 1-242 only.

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pEF1a-mRor2deltaIg

Plasmid

#22629

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 delta Ig (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of Ig domain

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
GAL 46Q+ProGFPp416

Plasmid

#15581

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q + Pro (HTT Human)

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pMig (Martins Lab)

Plasmid

#14125

Depositor

L. Miguel Martins

Article

Nicke et al Mol Cell. 2005 Dec 9. 20(5):673-85.

Insert

IRES-EGFP

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

Cla I sites were silenced in the original pRetroS…

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK2 L198A

Plasmid

#8975

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

L198A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pET28_mouse-pro-IL18

Plasmid

#183389

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

pro-IL18 (Il18 Mouse)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CAMK2D

Plasmid

#38987

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

CAMK2D (CAMK2D Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
CAMK2D

Plasmid

#38904

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

CAMK2D (CAMK2D Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pDONR223-CDK10

Plasmid

#23652

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDK10 (CDK10 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PCTK1

Plasmid

#23754

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PCTK1 (CDK16 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DGUOK

Plasmid

#23864

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DGUOK (DGUOK Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcdna3 T7 Jip2

Plasmid

#59028

Purpose

act as molecular scaffolds that mediate the activation of the JNK signaling pathway in vivo.

Depositor

Roger Davis

Article

Yasuda et al Mol Cell Biol. 1999 Oct;19(10):7245-54.

Insert

MAPK8IP2 mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2 Human)

Tags

T7

Expression

Mammalian

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pDONR223-DGKA

Plasmid

#23837

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DGKA (DGKA Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
PAK4-FL-3'UTR

Plasmid

#113088

Purpose

Luciferase reporter containing PAK4- FL-3'UTR

Depositor

James Donahue

Article

Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

Insert

p21 Activated kinase 4 (PAK4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

none

Promoter

PGK

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PdTK-CAG-mCh-[uBglII]

Plasmid

#107280

Purpose

puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

AAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-AGK

Plasmid

#23578

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AGK (AGK Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
L4552 x6NFATRE (NFAT) Ben DsRed-Express2 in TUPV1

Plasmid

#244201

Purpose

Expression of a DsRed-Express2 reporter under an NFAT-responsive promoter

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

NFAT-responsive promoter (6 NFAT binding sites + YB_TATA), dsRed-Express2 reporter

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304-DCK*-GW-IRES-GFP

Plasmid

#176289

Purpose

Gateway vector for use in generating DCK*-POI fusion reporter

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Insert

Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S74E R104M D133A

Promoter

CMV

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-2xFLAG-2xSTREP-CDK6

Plasmid

#172614

Purpose

Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK6 (CDK6 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-mEGFP(A206K)-paCaMKII(K42M)

Plasmid

#165440

Purpose

Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(K42M)

Tags

mEGFP(A206K)

Expression

Mammalian

Mutation

K42M: Kinase dead mutation

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p35S-A3A-PBE

Plasmid

#119773

Purpose

Conversion of C to T in potato

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS-UGI-NLS

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

CaMv 2x35S

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-CCR5

Plasmid

#98945

Purpose

Mammalian expression plasmid for untagged human CCR5

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-β-arrestin2 RRK/Q

Plasmid

#38261

Depositor

Robert Lefkowitz

Article

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Tags

Flag

Expression

Mammalian

Mutation

Arg233, Arg237, and Lys251 converted to Glutamines

Promoter

CMV

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
GST TopBP1 B (aa 978-1192)

Plasmid

#20372

Depositor

Aziz Sancar

Article

Choi et al Nucleic Acids Res. 2009 Apr;37(5):1501-9

Insert

TopBP1 (978-1192) (TOPBP1 Human)

Expression

Bacterial

Mutation

amino acids 978-1192

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO/Strep-HA-ZAKalpha_deltaCTD

Plasmid

#141196

Purpose

Expresses Strep-HA-ZAKalpha_deltaCTD in mammalian cells, can be used to make inducible cell lines

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 (MAP3K20 Human)

Tags

Strep, HA

Expression

Mammalian

Mutation

deletion of last 27 amino acids

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-MKK1

Plasmid

#107973

Purpose

expression of MAP Kinase Kinase

Depositor

Edwin Krebs

Article

Seger et al J Biol Chem. 1994 Oct 14;269(41):25699-7

Insert

MKK1 (MAP2K1 Human)

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

CMV

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/HisMaxB-YAP1-S164A

Plasmid

#18990

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein S164A mutant (YAP1 Human)

Tags

His

Expression

Mammalian

Mutation

Serine 164 changed to Alanine

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pET15TV-L-hlPYK WT

Plasmid

#187975

Purpose

Expresses the human liver pyruvate kinase

Depositor

Audrey Lamb

Article

McFarlane et al Acta Crystallogr F Struct Biol Commun. 2

Insert

hlPYK WT

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pB.FRB-3myc-KLC1a

Plasmid

#134627

Purpose

Mammalian expression of mouse KLC1a

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KLC1a (Klc1 Mouse)

Tags

FRB, 3myc

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
K401-iLid

Plasmid

#122484

Purpose

Component of optically-induced motor dimerization system

Depositor

Matt Thomson

Article

Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

Insert

Kinesin 1-401 iLid His tag

Tags

His6 and iLid

Expression

Bacterial

Mutation

Kinesin amino acids 1-401

Promoter

T7

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-CenpB 1-166

Plasmid

#108475

Purpose

expression of GFP-CenpB 1-166

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CenpB 1-166 AA (CENPB Human)

Tags

EGFP

Expression

Mammalian

Mutation

encodes only the DNA-binding domain of CENP-B

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-TTP(C147R)-GG-PUF

Plasmid

#52138

Purpose

Receiving vector for PUM-HD assembly and expression in mammalian cells

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Type

Empty backbone

Tags

(GGGGS)3GGGG linker, 3xFlag, PUM-HD flanking regi…

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-RPS6KA6

Plasmid

#23540

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KA6 (RPS6KA6 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKACG

Plasmid

#23788

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKACG (PRKACG Human)

Use

Gateway Cloning

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MYLK2

Plasmid

#23656

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MYLK2 (MYLK2 Human)

Use

Gateway Cloning

Mutation

P86A

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
PCTAIRE-1

Plasmid

#1996

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

PCTAIRE-1 (CDK16 Human)

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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