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We narrowed to 4,419 results for: crispr c plasmids

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Showing: 121 - 140 of 4419 results

  1. AAV-hSyn-mCherry-P2A-Cre-WPRE (AAV1)

    Viral prep
    #107312-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-hSyn-mCherry-P2A-Cre-WPRE (#107312). In addition to the viral particles, you will also receive purified AAV-hSyn-mCherry-P2A-Cre-WPRE plasmid DNA. hSyn-driven expression of mCherry and Cre (physically separate). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hui Yang
    Publication
    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/
    Tags
    mCherry (physically separate, not a fusion protein)
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  4. SapTrap-SEC kit for C. elegans genome engineering

    Plasmid kit
    #1000000109
    Purpose
    A collection of C. elegans plasmids used for building homologous repair templates that incorporate a self-excising drug selection cassette (SEC), based on the SapTrap CRISPR/Cas Toolkit.
    Depositor
    Bob Goldstein
    Application
    Genome Editing
    Vector type
    Worm Expression
    Editing type
    CRISPR
    Available since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  5. SP6-hCas9-Ce-mRNA

    Plasmid
    #47911
    Purpose
    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.
    Depositor
    Paul Sternberg
    Publication
    Chiu et al Genetics. 2013 Aug 26.
    Insert
    hCas9 with C. elegans 5' and 3' UTRs
    Use
    CRISPR
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    47911-plasmid-map-sequence-id-68528

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  9. pMB67

    Plasmid
    #47947
    Purpose
    Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter
    Depositor
    Mike Boxem
    Publication
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and egl-13 NLS
    Expression
    Worm
    Promoter
    hsp-16.48
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    47947-plasmid-map-sequence-id-68808

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  13. pMB63

    Plasmid
    #47945
    Purpose
    Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter
    Depositor
    Mike Boxem
    Publication
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and egl-13 NLS
    Expression
    Worm
    Promoter
    eef-1A.1 (eft-3)
    Available since
    Dec. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    47945-plasmid-map-sequence-id-68805

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  16. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid
    #160734
    Purpose
    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Publication
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    160734-plasmid-map-sequence-id-318595

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  19. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid
    #197511
    Purpose
    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and BPNLS-NpuC(intein)
    Mutation
    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…
    Promoter
    Cbh
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197511-plasmid-map-sequence-id-386647

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Showing: 121 - 140 of 4419 results