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We narrowed to 32,130 results for: por

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Showing: 2131 - 2160 of 32130 results

  1. pONSY-Venus

    Plasmid
    #111875
    Purpose
    This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Venus
    Use
    Capsaspora owczarzaki
    Tags
    Venus
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111875-plasmid-map-sequence-id-213485

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  2. pN1_Rev-GR(m10)-EGFP

    Plasmid
    #252751
    Purpose
    Control for studying nuclear export. Expression of a fusion protein containing full-length Rev, the hormone-responsive element of the rat Gr, and GFP. The m10 mutation abolishes nuclear export.
    Depositor
    Kristen Verhey
    Article
    Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do
    Insert
    HIV-1 Rev and Glucocorticoid Receptor (GR)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    m10 is mutation of nuclear export sequence
    Available Since
    March 19, 2026
    Availability
    Industry, Academic Institutions, and Nonprofits
    252751-plasmid-map-sequence-id-503504

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  10. WT MetNIQ in pBAD

    Plasmid
    #118253
    Purpose
    Wild Type Methionine Binding Protein and Methionine ABC Transporter in pBAD Vector for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala)
    Promoter
    None and araBAD Promoter
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    118253-plasmid-map-sequence-id-226828

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  11. pET-hMICU1EF

    Plasmid
    #199180
    Purpose
    This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.
    Depositor
    Ming-Feng Tsai
    Article
    Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;12
    Insert
    MICU1 (MICU1 Human)
    Tags
    His6 and MBP, Thrombin site, TEV site
    Mutation
    D421A, E432K, F433W, F453W
    Available Since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    199180-plasmid-map-sequence-id-403144

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  14. pBEST-p70a-UTR1-deGFP Y35TAG D193TAG-6xHis-T500

    Plasmid
    #92227
    Purpose
    Expression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    deGFP Y35TAG D193TAG
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Residues Y35 D193 were mutated into TAG (Amber) s…
    Promoter
    p70a
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    92227-plasmid-map-sequence-id-197047

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  19. pGF1-4eCOL2A1

    Plasmid
    #97210
    Purpose
    Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter
    Depositor
    Ryan Porter
    Article
    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d
    Insert
    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    COL2A1 regulatory elements
    Available Since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    97210-plasmid-map-sequence-id-182599

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  20. pELPS-eDHFR-YFP-T2A-Renilla

    Plasmid
    #193253
    Purpose
    Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    Depositor
    Mark Sellmyer
    Article
    Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1
    Insert
    The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    Use
    Lentiviral
    Tags
    Renilla luciferase (rLuc) and yellow fluorescent …
    Mutation
    None
    Promoter
    EF1a
    Available Since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    193253-plasmid-map-sequence-id-383064

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  21. pUC57-4eCOL2A1

    Plasmid
    #97211
    Purpose
    For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors
    Depositor
    Ryan Porter
    Article
    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d
    Insert
    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    Use
    Vector is designed for cloning and generation of …
    Promoter
    COL2A1 regulatory elements
    Available Since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    97211-plasmid-map-sequence-id-181460

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  23. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid
    #58477
    Purpose
    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Article
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed Arginine 62 to Aspartic Acid
    Promoter
    CMV
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    58477-plasmid-map-sequence-id-90386

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  29. pSin-EF2-DMD53-GFP

    Plasmid
    #138353
    Purpose
    Expressing a GFP reporter cassette, whose GFP reading frame is disrupted by insertion of partial DMD exon 53 sequences
    Depositor
    Baisong Lu
    Article
    Lyu et al PLoS One. 2020 Sep 24;15(9):e0239468. do
    Insert
    DMD exon 53 partial sequence to disrupt GFP reading frame (DMD Human)
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Promoter
    EF2
    Available Since
    Nov. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    138353-plasmid-map-sequence-id-273452

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  30. N229A (EcQ) MetNIQ in pBAD

    Plasmid
    #118257
    Purpose
    N229A Mutation in Methionine Binding Protein (MetQ) and Wild Type Methionine ABC Transporter for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (N229A) (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 229 to Alanine (Ala) and…
    Promoter
    None and araBAD Promoter
    Available Since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118257-plasmid-map-sequence-id-237124

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Showing: 2131 - 2160 of 32130 results