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pTRIP-CMV-mCherry-53BP1

Plasmid

#127658

Purpose

Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.

Depositor

Nicolas Manel

Publication

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

53BP1 (TP53BP1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

Nucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-NRG1B1

Plasmid

#120287

Purpose

Produce human recombinant NRG1 in DE3 cells

Depositor

Paul Burridge

Publication

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

NRG1-beta 1

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJYS3_ΔcrtYf

Plasmid

#85542

Purpose

Constitutive transcription of FnCpf1 and crRNA of crtYf

Depositor

Sheng Yang

Publication

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Inserts

Cpf1
crRNA of crtYf

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Expression

Bacterial

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

Plasmid

#79620

Purpose

Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Publication

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9

Tags

PmCDA1 and UGI

Expression

Mammalian

Mutation

D10A for nickase Cas9

Promoter

pCMV

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pSIN4-EF1a-ETV2-IRES-Puro

Plasmid

#61061

Purpose

lentiviral vector for ETV2

Depositor

Igor Slukvin

Publication

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

ETV2 (ETV2 Human)

Use

Lentiviral

Promoter

EF1alpha

Available since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

Plasmid

#49771

Purpose

Level 1 hCas9 module

Depositor

Sophien Kamoun

Publication

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

35Sp::hCas9

Use

CRISPR

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pET His6 GST TEV LIC cloning vector (2G-T)

Plasmid

#29707

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, GST, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pCMV p16 INK4A

Plasmid

#10916

Depositor

Bob Weinberg

Publication

Medema et al Proc Natl Acad Sci U S A. 1995 Jul 3. 92

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p16 INK4 (CDKN2A Human)

Expression

Mammalian

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-hIBA1a-GFP-miR124T

Plasmid

#214147

Purpose

AAV vector to restrict GFP expression in microglia.

Depositor

Chun-Li Zhang

Publication

Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

Has service

AAV5

Insert

GFP

Use

AAV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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B-95.ΔAΔfabR

Bacterial strain

#197934

Purpose

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Depositor

Kensaku Sakamoto

Publication

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Bacterial resistance

None

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Cas12f-GE ver4.1

Plasmid

#176544

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Publication

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas12f-GE ver4.1
Cas12f-GE ver4.1

Expression

Mammalian

Promoter

U6 and chicken β-actin

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-mDlx-GCaMP6f-Fishell-2 (AAV9)

Viral prep

#83899-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-mDlx-GCaMP6f-Fishell-2 (#83899). In addition to the viral particles, you will also receive purified pAAV-mDlx-GCaMP6f-Fishell-2 plasmid DNA. mDlx-driven expression of GCaMP6f. These AAV preparations are suitable purity for injection into animals.

Depositor

Gordon Fishell

Publication

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Promoter

mDlx

Available since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only
mNeonGreen-Giantin

Plasmid

#98880

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Giantin

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pKM464 (deletions)

Plasmid

#108322

Purpose

Orbit integrating plasmid to delete target gene.

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

Bxb1 attB site

Use

Orbit integration plasmid for creating knockouts …

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pC1-HyPer-C199S

Plasmid

#42213

Purpose

Peroxide-insensitive version of HyPer to be used as a pH-control. Can be applied as a pH sensor.

Depositor

Vsevolod Belousov

Publication

Belousov et al Nat Methods. 2006 Apr;3(4):281-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HYPER C199S

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMYC-GFP

Plasmid

#42142

Depositor

Olufunmilayo Olopade

Publication

Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

c-Myc (MYC Human)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hrGFP-Keap1

Plasmid

#28025

Depositor

Qing Zhong

Publication

Fan et al Autophagy. 2010 Jul 22. 6(5):.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Keap1 (KEAP1 Human)

Tags

hrGFP

Expression

Mammalian

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pMSCVpuro-Flag-cMyc T58A

Plasmid

#20076

Depositor

Juan Belmonte

Publication

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

cMyc (MYC Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

T58A

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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V51 pIRESpuro-GLUE (pGLUE)

Plasmid

#15100

Purpose

tandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tags

Depositor

Randall Moon

Publication

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMV5-AML1B

Plasmid

#12426

Purpose

pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

Depositor

Scott Hiebert

Publication

Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AML1B (RUNX1 Human)

Expression

Mammalian

Available since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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