We narrowed to 5,091 results for: codon optimized

1. pBad-sfGFP

   Plasmid

   #85482

   Purpose

   Arabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tag

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

   Insert

   superfolder GFP
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   Arabinose

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR-PE-bacteria

   Plasmid

   #172715

   Purpose

   Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   Cas9n-linker-M-MLV2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H840A of Cas9

   Promoter

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDe-CAS9

   Plasmid

   #61433

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PcUBI4-2

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p201H Cas9

   Plasmid

   #59176

   Purpose

   Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Inserts

   Cas9
   hph
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   2x35S and StUbi-3P

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDB179-ThermoPETase

   Plasmid

   #218698

   Purpose

   Encodes codon optimized ThermoPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   ThermoPETase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDB179-DepoPETase

   Plasmid

   #218702

   Purpose

   Encodes codon optimized DepoPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   DepoPETase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDB179-DuraPETase

   Plasmid

   #218699

   Purpose

   Encodes codon optimized DuraPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   DuraPETase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pN7HELIX

   Plasmid

   #190662

   Purpose

   Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   CMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. His-Tev-UbE1

   Plasmid

   #186804

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   His-Tev-UbE1 (UBA1 Human)
   

   Use

   Tags

   His6-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDB179-BhrPETase

    Plasmid

    #218692

    Purpose

    Encodes codon optimized BhrPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    BhrPETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDB179-CaPETaseM9

    Plasmid

    #218707

    Purpose

    Encodes codon optimized CaPETaseM9 enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    CaPETaseM9
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

    Plasmid

    #128424

    Purpose

    Human Codon Optimized dCas9-DNMT3A3L

    Depositor

    Bradley Bernstein

    Article

    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

    Insert

    dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7 Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9 (PX165)

    Plasmid

    #48137

    Purpose

    Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDSW1728

    Plasmid

    #120812

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)

    Depositor

    David S. Weiss, Craig Ellermeier

    Article

    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

    Insert

    red fluorescent protein (mCherry), codon-optimized for C. difficile
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPomyces-SaCas9

    Plasmid

    #129553

    Purpose

    Contains codon-optimized SaCas9 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    SaCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHCMV_RVFV_GP

    Plasmid

    #207304

    Purpose

    Mammalian expression of Rift valley fever virus GP protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    RVFV_GP (RVFV_sM_gp1 Rift valley fever virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPomyces-Sth1Cas9

    Plasmid

    #129552

    Purpose

    Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    Sth1Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDD282

    Plasmid

    #66823

    Purpose

    GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    GFP-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. mt POLRMT

    Plasmid

    #205059

    Purpose

    POLRMT protein expression

    Depositor

    Smita Patel

    Article

    Ramachandran et al Nucleic Acids Res. 2017 Jan 25;45(2):861

    Insert

    POLRMT (POLRMT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHCMV_EEE_E1E2

    Plasmid

    #207272

    Purpose

    Mammalian expression of Eastern equine encephalitis virus E1E2 protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    EEE_E1E2 (EEEVgp3 Eastern equine encephalitis virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDD268

    Plasmid

    #132523

    Purpose

    mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein, Daniel Dickinson

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    mNG-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized mNG

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

    Plasmid

    #31484

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    EF1s HA NLS Sce(opt) T2A IFP1.4
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    "Opt" indicates the I-SceI coding seque…

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pYZ960

    Plasmid

    #187972

    Purpose

    TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pN7CAST

    Plasmid

    #190661

    Purpose

    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJ204-loxP-100RPU.2-coEPO-lox2272

    Plasmid

    #154828

    Purpose

    Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietin

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Insert

    coEPO
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized (Cricetulus griseus)

    Promoter

    100RPU.2

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCRISPomyces-FnCpf1

    Plasmid

    #129554

    Purpose

    Contains codon-optimized FnCpf1 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    FnCpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PARP15-c020

    Plasmid

    #173101

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    N78-S267, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMAL-his-LbCpf1-EC

    Plasmid

    #79008

    Purpose

    Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    LbCpf1
    

    Use

    Tags

    MBP-6xHis-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PARP15-c021

    Plasmid

    #173102

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    G287-N470, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDHt/sk-PE

    Plasmid

    #92126

    Purpose

    Expression of codon-optimized Cas9-eGFP fusion protein with Ppdc promoter in Trichoderma reesei

    Depositor

    Zhihua Zhou

    Article

    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

    Insert

    Cas9-eGFP
    

    Use

    CRISPR; Trichoderma reesei expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Ppdc

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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