We narrowed to 29,684 results for: REP

1. pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

   Plasmid

   #55217

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)

   Plasmid

   #48297

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   StrepII and msfGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

   Plasmid

   #55216

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

   Plasmid

   #55221

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   StrepII and msfGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA

   Plasmid

   #183027

   Purpose

   B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Insert

   H2B-sfGFP-T2A-tdTomato
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

   Plasmid

   #55222

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)

   Plasmid

   #55223

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus replaced NBCe C-terminus (NT94)

   Plasmid

   #50912

   Purpose

   Expresses human NKCC1 with truncation of N-terminus, mutations at P676C I730C and C-terminus replaced with that of NBCe. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   P676C and I730C; N-term deletion of aa13-222; C-t…

   Promoter

   CMV

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease fusion with non cleavable linker (N-terminal Twin-Strep-tag)

   Plasmid

   #228661

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from Zika virus for biochemical assay

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Zika virus portion of NS2B protease fused to NS3
   

   Use

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. sgRNA pool for Streptococcus pneumoniae

    Pooled Library

    #170432

    Purpose

    CRISPR library targeting S. pneumoniae D39V

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107

    Expression

    Bacterial

    Use

    CRISPR

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

11. DNA repair library with 1,573 sgRNAs

    Pooled Library

    #177663

    Purpose

    CRISPR-interference (CRISPRi) library of 1,573 sgRNAs targeting 476 genes encoding factors involved in DNA repair and associated processes

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Species

    Homo sapiens

    Use

    CRISPR

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

12. DNA repair library with 1,513 sgRNAs

    Pooled Library

    #177670

    Purpose

    CRISPR-interference (CRISPRi) library designed to target 476 genes involved in DNA repair and associated processes. It contains 1,513 sgRNAs and 60 non-targeting control sgRNAs.

    Depositor

    Brittany Adamson

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

13. DNA repair library with 336 sgRNAs

    Pooled Library

    #177664

    Purpose

    CRISPR-interference (CRISPRi) library of 366 sgRNAs targeting 118 genes encoding factors involved in DNA repair and associated processes

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Species

    Homo sapiens

    Use

    CRISPR

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM)

    Pooled Library

    #198050

    Purpose

    By supplying an AAV cap gene of interest in trans, researchers can use this library to investigate the effects of rep mutations on production of a wide range of AAV capsid serotypes

    Depositor

    George Church

    Article

    Jain et al bioRxiv 2023.01.31.526541

    Expression

    Mammalian

    Use

    AAV

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

15. pRep-Cap Scanning Saturation Mutagenesis (SSM) pooled library

    Pooled Library

    #199601

    Purpose

    pRep-Cap SSM library enables VP1, VP2, and VP3 expression under the control of the endogenous p40 promoter

    Depositor

    George Church

    Article

    Ogden et al Science. 2019 Nov 29;366(6469):1139-1143

    Expression

    Mammalian

    Species

    Aav2

    Use

    AAV

    Available since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

16. Anti-Strep [IPI-C23.21] (Recombinant Antibody)

    Antibody

    #218105-rAb

    Purpose

    Anti-Strep tag chimeric recombinant antibody, with fused mouse variable and rabbit constant domains; binds to WSHPQFEK sequence.

    Depositor

    Institute for Protein Innovation (IPI)

    Article

    Institute for Protein et al Unpublished

    Recommended Applications

    Western Blot

    Reactivity

    Source Species

    Rabbit

    Isotype

    IgG1

    Trial Size

    Not available to purchase

    Available since

    May 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

17. CoV-NSP3-c001

    Plasmid

    #173082

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E1000-K1173, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRT

    Plasmid

    #120274

    Purpose

    TA-cloning vector of PCR-products

    Depositor

    Ken Motohashi

    Article

    Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CoV-2-NSP3-c001

    Plasmid

    #173083

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E1084-E1192, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CoV-2-NSP3-c002

    Plasmid

    #173084

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E1084-E1192, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge