We narrowed to 7,972 results for: lif
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Plasmid#123350PurposeYellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.DepositorInsertVenus-cpVenus-FLARE-Cameleon
Tags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-cpVenus-FLARE-ICUE
Plasmid#123355PurposeYellow-fluorescent homoFRET/anisotropy-based cAMP indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.DepositorInsertVenus-cpVenus-FLARE-ICUE
Tags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianPromoterCMVAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL11060
Plasmid#68264PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)DepositorInsertPromoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
UseSynthetic BiologyExpressionPlantAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-mRuby3-CERT(PHD)
Plasmid#221762PurposeStable expression of mRuby3-CERT(PHD) in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationamino acids 1–116Available SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; HE1A:BFP -0
Plasmid#180010PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; HE1A: BFP
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-PPARG
Plasmid#192912PurposeBarcoded piggybac transposon vector with Dox-inducible expression of PPARGDepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human full-length LIC1
Plasmid#74597Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tagDepositorInsertfull length human dynein light intermediate chain 1 (DYNC1LI1 Human)
TagsN-terminal GST and Strep IIExpressionBacterialPromoterT7Available SinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-WT1
Plasmid#192906PurposeBarcoded piggybac transposon vector with Dox-inducible expression of WT1DepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1cam-Fc-His
Plasmid#72083PurposeExpresses the extracellular region of the L1CAM protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-cpVenus-FLARE-AKAR
Plasmid#123329PurposeYellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.DepositorInsertVenus-cpVenus-FLARE-AKAR
Tags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianPromoterCMVAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)
Plasmid#123330PurposeNegative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.DepositorInsertVenus-cpVenus-FLARE-AKAR(T/A)
Tags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; HE1A:BFP -1
Plasmid#180011PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; HE1A: BFP
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus-tBid-pEGFP-C1
Plasmid#166736PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, tBidDepositorAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl-2-s2193
Plasmid#166750PurposeExpress mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2 in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.DepositorAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 mitoAURKA
Plasmid#157750PurposeExpression of AURKA targeted to mitochondriaDepositorInsertAURKA (AURKA Human)
ExpressionMammalianMutationAurora A without the first 30AA + MTS of CoxVIIIPromoterCMVAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-NR1H2
Plasmid#192919PurposeBarcoded piggybac transposon vector with Dox-inducible expression of NR1H2DepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-H2B-V5-mScarlet
Plasmid#191093PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertH2B and mScarlet
UseAAVTagsH2B-V5-mScarletExpressionMammalianMutationThe fusion protein was optimized to the Human cod…PromoterCMV and CAGAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-OSBP(PHD)
Plasmid#221745PurposeStable expression of GFP-OSBP(PHD) in mammalian cellsDepositorAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-EF1a-mTagRFP-T-CAAX
Plasmid#167973PurposeAAVS1 targeting repair templateDepositorUseCRISPR; Donor templateTagsmTagRFP-T-CAAXExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair template
Plasmid#167943PurposeSFTPC reporter targeting repair templateDepositorInsertSFTPC (SFTPC Human)
UseCRISPR; Donor templateTagsVenusExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterEF1aAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
iSH-iRFP670-sspB
Plasmid#176102PurposeHeterodimerization with iLID, phosphatidylinositol 3-kinase derived iSHDepositorInsertphosphatidylinositol 3-kinase (Pik3r1 Mouse)
ExpressionMammalianAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-T2A-mCherry-PTS1
Plasmid#87827PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
TagsEGFP and mCherryExpressionMammalianPromoterCMV promoter, tetracycline operatorAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bad-pEGFP-C1
Plasmid#166733PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, BadDepositorAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-CD8-NbVHH05-GFP
Plasmid#171576PurposeExpresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembraneDepositorInsertCD8-NbVHH05-GFP (UBE2J1 Human)
TagsCD8 and GFPExpressionInsectPromoterfly actin5C promoterAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flrt2-Fc-His
Plasmid#72074PurposeExpresses the extracellular region of the FLRT2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-Necl-5-ScNeo
Plasmid#170283PurposeTo monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInserta recombinant mouse Necl-5 fused to the mScarlet and to the mNeonGreen fluorescent protein (Pvr Synthetic, Mouse)
TagsmScarlet and mNeonGreenExpressionMammalianAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Anti-PICK1 [L20/8R]
Plasmid#128630PurposeMammalian Expression Plasmid of anti-PICK1 (Human). Derived from hybridoma L20/8.DepositorInsertanti-PICK1 (Human) recombinant mouse monoclonal antibody (PICK1 Mouse)
ExpressionMammalianPromoterDual CMVAvailable SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGPN-ACE2
Plasmid#187483PurposeExpression vector - ACE2DepositorAvailable SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Atoh1
Plasmid#158529PurposeExpresses mouse Atoh1 in mammalian cellsDepositorAvailable SinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag
Plasmid#185682PurposeLow-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.DepositorAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Anti-Neuroligin-1 [N97A/31R]
Plasmid#114554PurposeMammalian Expression Plasmid of anti-Neuroligin-1 (Rat). Derived from hybridoma N97A/31.DepositorInsertanti-Neuroligin-1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn1 Mouse)
ExpressionMammalianPromoterdual CMVAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV superYFP-AURKA-mTurq2
Plasmid#157772PurposeExpression of AuroraA kinase biosensor under CMV promoterDepositorAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-TSC22D3
Plasmid#192916PurposeBarcoded piggybac transposon vector with Dox-inducible expression of TSC22D3DepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lrrc4c-Fc-His
Plasmid#72085PurposeExpresses the extracellular region of the LRRC4C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
kanMX6-ins6
Plasmid#195043PurposepFA6a derived selection cassette 5' flanked with tDEG1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interferenceDepositorInsertKanR
UseYeast genomic targetingTagsS. cerevisiae DEG1 terminator, A. gossypii TEF pr…Available SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-mScarlet-LZ-LOV2
Plasmid#197419PurposeMammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1DepositorInsertMAPRE1 (MAPRE1 Human)
TagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…Available SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CMV-Grid2(I677C)
Plasmid#164782Purposecysteine mutated glutamate delta2 receptor (I677C) for the attachment of a photoswitchable tethered ligandDepositorAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL-pEGFP-C1
Plasmid#166735PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, BimELDepositorAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-AC8(1-106)
Plasmid#181958PurposeResidues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.DepositorInsertEGFP-AC8(1-106) (Adcy8 Rat)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 mCherry-Rtn4HD
Plasmid#86685PurposeLentivirus to stably express fluorescent human protein in mammalian cellsDepositorInsertRtn4 homology domain (RTN4 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationhomology domain onlyPromoterCMVAvailable SinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only