We narrowed to 54,252 results for: plasmid

1. pAS2-his-24::Cerulean

   Plasmid

   #124324

   Purpose

   Expressing Nuclear Localized Cerulean

   Depositor

   Andrew Leifer

   Insert

   Cerulean (his-24 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS2-his-24::tagBFP2

   Plasmid

   #124322

   Purpose

   Expressing Nuclear Localized tagBFP2

   Depositor

   Andrew Leifer

   Insert

   tagBFP2 (his-24 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PG1424PGA

   Plasmid

   #74653

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PG1424PGA
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. psicheck_85_CA_repeat

   Plasmid

   #65881

   Purpose

   Luciferase assay vector, assessing regulation of 85 nucleotide CA repeats

   Depositor

   Thomas Tuschl

   Insert

   CA repeats 85 nucleotides in length
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

5. psicheck_SRM1_3UTR

   Plasmid

   #65883

   Purpose

   Luciferase assay vector, assessing regulation of SRM1 3' UTR

   Depositor

   Thomas Tuschl

   Insert

   SRM1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

6. Mouse 3' Ubf AID GFP PuroR

   Plasmid

   #127901

   Purpose

   HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Ubf Gene

   Depositor

   Mark Groudine

   Insert

   Ubf (Ubtf Mouse, Mustard Weed)
   

   Use

   Donor plasmid

   Tags

   GFP, AID, PuroR

   Expression

   Mutation

   Inserting GFP AID 2A Puro into mouse 3' Ubf

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJAT32

   Plasmid

   #204297

   Purpose

   pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-Clover-HIF-1alpha Wildtype

   Plasmid

   #163365

   Purpose

   Express HA- and Clover-tagged HIF-1alpha wildtype plasmid

   Depositor

   Violaine Sée

   Insert

   Hypoxia Inducible Factor 1 alpha (HIF1A Human)
   

   Use

   Tags

   HA- and Clover-tagged

   Expression

   Mammalian

   Mutation

   Wildtype

   Promoter

   CMV Promoter

   Available since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Spike

   Plasmid

   #173921

   Purpose

   Expresses the original SARS-Cov-2 Spike Protein under a CMV Promoter

   Depositor

   Andreas Stuermer

   Insert

   SARS-2 Spike Protein (S Synthetic, Human, SARS-COV-2)
   

   Use

   Tags

   no tags, native protein

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   standard CMV

   Available since

   April 11, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pU6-Universal

    Plasmid

    #52694

    Purpose

    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

    Depositor

    Sebastian Lourido

    Inserts

    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    

    Use

    CRISPR; Toxoplasma gondii

    Tags

    3X FLAG and Nuclear localization signal

    Expression

    Mutation

    Promoter

    TgTUB1 and Toxoplasma U6

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJAT77

    Plasmid

    #204295

    Purpose

    pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid

    #159908

    Purpose

    Mutagenesis of Htr2a

    Depositor

    Larry Zweifel

    Insert

    Htr2a (Htr2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7RNAP-Q737P

    Plasmid

    #175356

    Purpose

    Support plasmid for T7_ori plasmids

    Depositor

    Sven Panke

    Article

    Becker et al Nucleic Acids Res. 2021 Jul 13. pii: 632

    Insert

    T7RNAP-Q737P
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q737P

    Promoter

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM/SAR-CAG-aSyn(A53T)/HA-WPRE-bGHpA

    Plasmid

    #194242

    Purpose

    Expresses human alpha-Synuclein A53T

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    aSynuclein(A53T) (SNCA Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    A53T

    Promoter

    SAR-CAG

    Available since

    Jan. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pGL4.10/RSV_XS3B-miniXon_Luciferase

    Plasmid

    #217302

    Purpose

    Plasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a modification of the SF3B3-miniXon system. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-XS3B by L

    Depositor

    Beverly L. Davidson

    Insert

    XS3B-miniXon
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Kozak ATG initiation sequence in middle exon

    Promoter

    RSV

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pESC-MET

    Plasmid

    #222482

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and MET15 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pESC-LYS

    Plasmid

    #222483

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and LYS2 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pESC-TYR

    Plasmid

    #222484

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and TYR1 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pESC-THR

    Plasmid

    #222479

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and THR1 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pESC-ADE

    Plasmid

    #222480

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and ADE2 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge