We narrowed to 18,992 results for: Met;

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TN-XXL pcDNA3

Plasmid

#45797

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Insert

TN-XXL

Tags

ECFP and YFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only
pMK375 (Hygro-P2A-Stag-3FLAG)

Plasmid

#121186

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-Stag-3FLAG

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2 3xmiR-122T

Plasmid

#35672

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

3 miR-122 target sites

Use

Luciferase

Promoter

HSV-TK

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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UMG-SP-1_pET29b

Plasmid

#215433

Purpose

Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-1

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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UMG-SP-2_pET29b

Plasmid

#215434

Purpose

Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-2

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-C4-mCherry

Plasmid

#66530

Purpose

Expresses mCherry under the control of Mutant T7 Promoter C4

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - C4

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pEB2-TagRFP

Plasmid

#103997

Purpose

Plasmid encoding TagRFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

TagRFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, K42R, K67R, …

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pU1/Sm/3' Box_(GLuc)_TOP1

Plasmid

#68437

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U1

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pREF-GFP kan

Plasmid

#122061

Purpose

reporter plasmid for termination and terminator override to express GFP. Marked with URA3

Depositor

Daniel Reines

Article

Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

Inserts

intergenic terminator from yeast IMD2
green fluorescent protein

Expression

Yeast

Promoter

GAL1 and GAL1 comes in pYES2

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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mIFP24-C1

Plasmid

#54820

Purpose

Localization: C1 Cloning Vector, Excitation: 683, Emission: 704

Depositor

Michael Davidson, Xiaokun Shu

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Type

Empty backbone

Tags

mIFP24

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET28a-ybbR-HIS-sfGFP-DocI

Plasmid

#58708

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

sfGFP-DocI

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Promoter

T7

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pmScarlet-H_peroxisome_C1

Plasmid

#85064

Purpose

In vivo visualization of the peroxisome (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Peroxisomal targeting sequence

Tags

mScarlet-H

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Vinculin-23

Plasmid

#58030

Purpose

Localization: Focal Adhesions, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Vinculin (VCL Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
AAV-CamK2-tTA

Plasmid

#210731

Purpose

AAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)

Depositor

Wei Xu

Article

Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

tetracycline-controlled transactivator (tTA)

Use

AAV

Promoter

CamK2

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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e-ciCas9_pcDNA5

Plasmid

#100553

Purpose

Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

e-ciCas9

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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Clover-Rab5a-7

Plasmid

#56530

Purpose

Localization: Ras GTPase, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Rab5a

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pKMV-VioC

Plasmid

#65349

Purpose

systhesis of vioC gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioC

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pTRE2-Hyg-omMx1

Plasmid

#52420

Purpose

Inducible expression (Tet-Off system) of the rainbow trout antiviral Mx1 protein

Depositor

Bertrand Collet

Article

Lester et al J Virol Methods. 2012 Jun;182(1-2):1-8.

Insert

Oncorhynchus mykiss interferon-induced GTP-binding protein Mx1 (mx1)

Expression

Mammalian

Promoter

TRE2

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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mRuby2-Cx43-7

Plasmid

#55894

Purpose

Localization: Gap Junctions, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Cx43

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pUAST cyto roGFP2-Orp1

Plasmid

#64996

Purpose

for expression of cytosolic roGFP2-Orp1 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Tags

roGFP2

Expression

Insect

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano30/pcDNA3

Plasmid

#51963

Purpose

mammalian expression of Yellow Cameleon-Nano30, an ultrasensitive Ca2+ indicator with Kd = 30 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano30

Expression

Mammalian

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
pEB1-mCerulean ME

Plasmid

#103971

Purpose

Plasmid encoding mCerulean ME

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCerulean ME

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_INT[Spinach2]

Plasmid

#68430

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing the "Spinach2" aptamer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV/3' Box_(GLuc)_TOP2

Plasmid

#68435

Purpose

Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP2 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMBA323

Plasmid

#214751

Purpose

To create delta(glmS,nadE) working strains and maintaining their viability

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)

Use

CRISPR

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA-mCtnnb1

Plasmid

#84542

Purpose

mCtnnb1 gRNA expressing vector

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

mCtnnb1 gRNA

Use

CRISPR

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM2937

Plasmid

#84112

Purpose

target miRNA2937 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM2937

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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mOrange2-H2B-6

Plasmid

#57962

Purpose

Localization: Nucleus/Histones, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Insert

H2B (H2BC11 Human)

Tags

mOrange2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
SBP-mCherry-Ecadherin_puromycin

Plasmid

#65293

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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mRuby2-MannII-N-10

Plasmid

#55903

Purpose

Localization: Mannosidase/Golgi, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

MannII

Tags

mRuby2

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Str-KDEL_flGalT-SBP-tagBFP

Plasmid

#65274

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pWKS1583

Plasmid

#70188

Purpose

Modular vector for secreted amylase reporter expression

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

amyEopt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pFU-no toxin-PE

Plasmid

#24149

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

Insert

PDGF-receptor-transmembrane domain (PDGFRB Aequorea victoria, Human)

Use

Lentiviral

Tags

EGFP and FLAG

Expression

Mammalian

Available Since

April 8, 2010

Availability

Academic Institutions and Nonprofits only
ManII-SBP-EGFP_puromycin

Plasmid

#65260

Purpose

ManII reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated Mannosidae II

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pKMV-VioE

Plasmid

#65351

Purpose

systhesis of vioE gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioE

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM159

Plasmid

#84084

Purpose

target miRNA159 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM159

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mOrange-H2B-6

Plasmid

#57942

Purpose

Localization: Nucleus/Histones, Excitation: 548, Emission: 562

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Insert

H2B (H2BC11 Human)

Tags

mOrange

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pDS272

Plasmid

#34975

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2-GFP-LOVpep (MID2 A. sativa, Budding Yeast)

Expression

Yeast

Mutation

T406A, T407A

Promoter

TEF

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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Syt1LeftArm-3XGS-GCaMP6s-SV40-3XP3-dsRed-SV40-Syt1RightArm

Plasmid

#159636

Purpose

Donor plasmid for generating Syt1:GCaMP6s strain in Aedes aegypti with CRISPR/Cas9

Depositor

Carolyn McBride

Article

Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

Insert

GCaMP6s

Expression

Insect

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ1202

Plasmid

#44484

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, peel-1, MCS

Use

Multiple cloning site vector

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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