Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

171,702 results

Showing: 3801 - 3850 of 171702 results

  3. pCas

    Plasmid
    #62225
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgR
    Depositor
    Sheng Yang
    Article
    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii
    Has Service
    Cloning Grade DNA
    Insert
    cas9
    Use
    CRISPR; Lambda red recombinase
    Expression
    Bacterial
    Promoter
    native cas9 promoter
    Available Since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    62225-plasmid-map-sequence-id-275267

    Enlarge

  5. pAAV-hSyn-DIO-EGFP (AAV5)

    Viral Prep
    #50457-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available Since
    July 8, 2019
    Availability
    Academic Institutions and Nonprofits only

  7. pCV020-tetQ

    Plasmid
    #232826
    Purpose
    Random transposon mutagenesis vector for Bacteroidales species - tetracycline
    Depositor
    Athanasios Typas
    Insert
    tetQ
    Expression
    Bacterial
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232826-plasmid-map-sequence-id-462958

    Enlarge

  8. Chromoprotein kit 2.0

    Plasmid Kit
    #1000000198
    Purpose
    Expression of visible chromoproteins in E. coli, including two new chromoprotein genes and improved stability.
    Depositor
    Anthony Forster
    Application
    For the Classroom, Gene Expression and Labeling
    Vector Type
    Bacterial Expression
    Available Since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only

  9. AAV-CamKIIa-jGCaMP8s-WPRE (AAV1)

    Viral Prep
    #176752-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-CamKIIa-jGCaMP8s-WPRE (#176752). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8s-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    CamKIIalpha
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only

  11. pAAV-syn-flex-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234442
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Has Service
    AAV1
    Insert
    iGluSnFR4f-NGR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234442-plasmid-map-sequence-id-465693

    Enlarge

  14. pcDNA3.3_CoV2_D18

    Plasmid
    #170442
    Purpose
    Express SARS-CoV-2 WT spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Rogers et al Science. 2020 Aug 21;369(6506):956-963.
    Insert
    SARS-CoV-2 spike D18 (S SARS-CoV-2, USA-WA1/2020 strain)
    Tags
    NA
    Expression
    Mammalian
    Mutation
    18aa deletion in c-terminal tail
    Promoter
    CMV
    Available Since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    170442-plasmid-map-sequence-id-337888

    Enlarge

  23. pcDNA3-TLR4-YFP

    Plasmid
    #13018
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 4 (TLR4 Human)
    Tags
    YFP
    Expression
    Mammalian
    Available Since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13018-plasmid-map-sequence-id-339915

    Enlarge

  25. pLIX403-ccdB-Blast

    Plasmid
    #158560
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158560-plasmid-map-sequence-id-309876

    Enlarge

  27. pAAV_BiPVe3_GCaMP6f (AAV1)

    Viral Prep
    #213943-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV_BiPVe3_GCaMP6f (#213943). In addition to the viral particles, you will also receive purified pAAV_BiPVe3_GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the PV+ basket cell-targeting enhancer E3. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1
    Available Since
    Sept. 23, 2025
    Availability
    Academic Institutions and Nonprofits only

  28. pET22b_PstS_2

    Plasmid
    #78199
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A17C,A197C)
    Expression
    Bacterial
    Mutation
    A17C; A197C (please see depositor comment below)
    Promoter
    T7
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78199-plasmid-map-sequence-id-146049

    Enlarge

  30. pENN.AAV.CMVs.Pl.Cre.rBG (AAV5)

    Viral Prep
    #105537-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only

  33. pAAV-CaMKIIa-Flpo (AAV8)

    Viral Prep
    #183535-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-Flpo (#183535). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-Flpo plasmid DNA. CamKIIa-driven expression of Flpo recombinase. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi
    Promoter
    CaMKII
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only

  34. dSaCas9-VPR

    Plasmid
    #205251
    Purpose
    In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495
    Depositor
    Rasmus Bak
    Article
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    dSaCas9-VPR
    Use
    CRISPR
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    205251-plasmid-map-sequence-id-408439

    Enlarge

  43. pOpen-MMLV_RT (lack H)

    Plasmid
    #165556
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165556-plasmid-map-sequence-id-339910

    Enlarge

  47. EGFP-GRAM-W

    Plasmid
    #211701
    Purpose
    Expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (EGFP-GRAM1b G187W) in mammalian cells
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211701-plasmid-map-sequence-id-419225

    Enlarge

Showing: 3801 - 3850 of 171702 results