168,515 results

1. pQE30-roGFP1-iR

   Plasmid

   #83314

   Purpose

   Inducible expression of redox sensitive GFP in bacteria

   Depositor

   S. James Remington

   Article

   Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

   Insert

   roGFP1-iR
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

   Promoter

   T5

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pSTARR-seq_fly

   Plasmid

   #71499

   Purpose

   flySTARR-seq screening vector with DSCP core promoter.

   Depositor

   Alexander Stark

   Article

   Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

   Type

   Empty backbone

   Use

   Flystarr-seq screening vector

   Tags

   sgGFP (SuperGlo, Qbiogene, Inc)

   Expression

   Insect

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPK2660

   Plasmid

   #70709

   Purpose

   Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_BiPVe4_dTomato_nlsdTomato

   Plasmid

   #213936

   Purpose

   AAV construct expressing dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_dTomato_nlsdTomato

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Has Service

   AAV PHP.eB and AAV1

   Insert

   dTomato_P2A_nls.dTomato
   

   Use

   AAV

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-N SV40 LT

   Plasmid

   #37857

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   SV40 LT (SV40gp6 Simian Virus 40)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   PKG

   Available Since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. p6352 MSCV-CMV-CMV-Flag-HA-JMJD6

   Plasmid

   #31358

   Depositor

   Peter Howley

   Article

   Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

   Insert

   JMJD6 (JMJD6 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Available Since

   Oct. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV hSyn FLEx-loxp Kir2.1-2A-GFP

   Plasmid

   #161574

   Purpose

   Expression of Kir2.1-2A-GFP in a Cre-dependent fashion

   Depositor

   Kevin Beier, Robert Malenka

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   Kir2.1-2A-GFP (Kcnj2 Mouse)
   

   Use

   AAV

   Mutation

   NA

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM5505

   Plasmid

   #132664

   Purpose

   pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Insert

   MobA
   

   Expression

   Bacterial

   Mutation

   please see depositor comments below

   Promoter

   p1,p3

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNLF1-C_PLCG1:1-1291

   Plasmid

   #211089

   Purpose

   Mammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   PLCG1:M1-L1291 (PLCG1 Human)
   

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   isoform 2. I813T natural variant (VAR_011908)

   Promoter

   CMV

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM11-6xHis-TEV-hORF1p

    Plasmid

    #202589

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Kathleen Burns, Liam Holt

    Article

    Taylor et al Cell. 2013 Nov 21;155(5):1034-48. doi: 1

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Available Since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #236406

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNuc-TrxRFP1

    Plasmid

    #98998

    Purpose

    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization

    Depositor

    Huiwang Ai

    Article

    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

    Insert

    TrxRFP1 and nuclear localization sequence
    

    Tags

    His-tag and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. (559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

    Plasmid

    #163024

    Purpose

    Expression of CRISPRi with U6-gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    AAV

    Tags

    KRAB, SV40 polyA, and myc NLS

    Expression

    Mammalian

    Promoter

    hEF-1a

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMVV052

    Plasmid

    #215303

    Purpose

    Expresses sfGFP under rhamnose induction (RhaS + RhaR + pRhaB-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Promoter

    PRhaB

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid

    #139988

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLPC-Flag-PML-IV

    Plasmid

    #62804

    Purpose

    expresses the isoform IV of PML (promyelocytic leukemia protein)

    Depositor

    Gerardo Ferbeyre

    Article

    Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

    Insert

    PML-IV (PML Human)
    

    Use

    Retroviral

    Tags

    3X-FLAG

    Expression

    Mammalian

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pAC026

    Plasmid

    #227598

    Purpose

    Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

    Depositor

    Brittany Adamson

    Article

    Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. p_m16S

    Plasmid

    #160549

    Purpose

    Used for mycoplasma detection by qPCR. 16S insert truncated internally from the strain Mycoplasma capricolum subsp. capricolum strain California Kid. ATB: Carbenicilline

    Depositor

    Denis Gerlier

    Article

    Jean et al PLoS One. 2017 Feb 22;12(2):e0172358. do

    Insert

    16S rDNA fragment from M. capricolum subsp. capricolum strain California Kid (gi_83283139)
    

    Use

    Unspecified

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pV1393

    Plasmid

    #111430

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV::TVA/tRFP

    Plasmid

    #164486

    Purpose

    Dual CMV promoter plasmid to express TVA and TurboRFP

    Depositor

    Hollis Cline

    Article

    Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

    Inserts

    TVA (CD320 Chicken)
    TurboRFP
    

    Expression

    Mammalian

    Promoter

    sCMV and sCMV IE94

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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