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Plasmid#58935Purposelabels macrophages with mcherryDepositorInsertmpeg1-mcherry
UseTagsExpressionMutationPromotermpeg1Available sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRG2
Plasmid#104174PurposesgRNA expressionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOpen-DpnIR
Plasmid#165529PurposeUsed to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)DepositorInsertDpnI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
hCtRNA_FT
Plasmid#186715PurposeFragment template for DAP arrayDepositorInsertgRNA scaffold and hCtRNA
UseCRISPRTagsExpressionMammalianMutationPromoterhCtRNAAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgG1/λ
Plasmid#50366PurposeExpresses grass pollen allergen Phl p 7 specific human IgG1/λ antibody isotype (102.1F10-IgG1/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Gamma 1 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
UseTagsExpressionMammalianMutationPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-SEAP-26Sfur-B2AD-tevs-KKMP
Plasmid#179636PurposeTEVP-inducible single TM SEAP RELEASE constructDepositorInsertSEAP RELEASE
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV1)
Viral Prep#112677-AAV1PurposeReady-to-use AAV1 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpRY Cas9
Plasmid#188491PurposeExpresses FLAG tagged SpRY Cas9DepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationA61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…PromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK FKBP12(F36V)-OGT (Neo)
Plasmid#154294PurposeLentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoterDepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x HA and FKBP12(F36V)ExpressionMammalianMutationPromoterPGKAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_eGFP
Plasmid#26822DepositorInsert5'UTR-eGFP-3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-HA-rat Nrxn1alpha
Plasmid#58266PurposeExpression plasmid for HA-tagged rat Neurexin 1alphaDepositorInsertNrxn1alpha (Nrxn1 Rat)
UseTagsHAExpressionMammalianMutationsee commentsPromoterCAGAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHDR_sox2HA_P2A-GFP-pA_HA_G2913A
Plasmid#196191PurposeTagging human SOX2 gene with P2A-EGFP at the C-terminusDepositorInsertSOX2-HAL-P2A-EGFP-SOX2-HAR (SOX2 Human)
UseHomology repair donorTagsExpressionMutationG2913A mutation to replace the guide cutting site…PromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.GFP
Plasmid#57827PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
EF1a_SPI1_P2A_Hygro_Barcode
Plasmid#120482PurposeBarcoded lentiviral vector to express SPI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertSPI1 (SPI1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMK001
Plasmid#33780DepositorInsertProteorhodopsin Optical Proton Sensor
UseTagsExpressionBacterialMutationD97NPromoterAraBADAvailable sinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMKV058
Plasmid#133313PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and WUS2DepositorInsertLuc+. WUS2
UseTagsExpressionPlantMutationPromoterAtUbi10, NosAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgE/λ
Plasmid#50365PurposeExpresses grass pollen allergen Phl p 7 specific human IgE/λ antibody isotype (102.1F10-IgE/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human epsilon heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
UseTagsExpressionMammalianMutationPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
P2X4-pHluorin123
Plasmid#52926Purposeexpress P2X4 protein with pHluorin at 123rd positionDepositorInsertmP2X4-pHluorin123
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV-MYC-t2A-BCL2
Plasmid#135306PurposeOverexpression of multiple human genes (BCL2 and MYC)DepositorUseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-GFP-ECL-3TM-tevs-KKMP V3
Plasmid#179640PurposeTEVP-inducible membrane-bound GFP RELEASE constructDepositorInsertMembrane-bound GFP RELEASE
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only