Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

175,655 results

Showing: 3901 - 3950 of 175655 results

  1. pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657

    Plasmid
    #212961
    Purpose
    Lentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_backbone
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212961-plasmid-map-sequence-id-422482

    Enlarge

  3. pAAV-CaMKIIa-EGFP (AAV9)

    Viral Prep
    #50469-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-EGFP (#50469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-EGFP plasmid DNA. CamKIIa-driven EGFP expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    CaMKII
    Tags
    EGFP
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  5. RXFP1-Tango

    Plasmid
    #66492
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    RXFP1 (RXFP1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pTRKH2

    Plasmid
    #71312
    Purpose
    Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.
    Depositor
    Rodolphe Barrangou, Todd Klaenhammer
    Article
    O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.
    Type
    Empty backbone
    Use
    E. coli-gram positive bacteria shuttle vector
    Expression
    Bacterial
    Available Since
    Jan. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    71312-plasmid-map-sequence-id-323567

    Enlarge

  11. TFORF1974

    Plasmid
    #143988
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TBX3 (TBX3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143988-plasmid-map-sequence-id-385902

    Enlarge

  15. pOPARA2

    Plasmid
    #200417
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200417-plasmid-map-sequence-id-394144

    Enlarge

  21. Lenti-multi-Guide

    Plasmid
    #85401
    Purpose
    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo
    Depositor
    Qin Yan
    Article
    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Feb. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    85401-plasmid-map-sequence-id-322305

    Enlarge

  25. pOPARA1

    Plasmid
    #200416
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200416-plasmid-map-sequence-id-394143

    Enlarge

  32. FUGW-APPSwe-T2A-Hygro

    Plasmid
    #190805
    Purpose
    Express Swedish mutant APP from Ubiquitin C promoter, co-expressing Hygro resistance gene
    Depositor
    Ronald Hart
    Insert
    APP (APP Human)
    Use
    Lentiviral
    Mutation
    Swedish (KM670/671NL)
    Promoter
    Ubi C
    Available Since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    190805-plasmid-map-sequence-id-372847

    Enlarge

  39. pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40 (AAV1)

    Viral Prep
    #101064-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40 (#101064). In addition to the viral particles, you will also receive purified pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40 plasmid DNA. Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator his-tagged CaMPARI2-L398T. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Eric Schreiter
    Article
    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:
    Promoter
    Syn
    Available Since
    July 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  45. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid
    #140003
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140003-plasmid-map-sequence-id-337354

    Enlarge

  46. pET50_WT MDA5

    Plasmid
    #216802
    Purpose
    Expresses wild type (wt) melanoma differentiation-associated protein 5
    Depositor
    Sun Hur
    Insert
    melanoma differentiation-associated protein 5 (IFIH1 Human)
    Expression
    Bacterial
    Available Since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    216802-plasmid-map-sequence-id-428834

    Enlarge

  48. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q79

    Plasmid
    #111729
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q79 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111729-plasmid-map-sequence-id-226812

    Enlarge

Showing: 3901 - 3950 of 175655 results