Skip to main content
Addgene
Showing: 351 - 400 of 471 results
  1. pJR021

    Plasmid
    #198814
    Purpose
    Variant of yellow fluorescent protein for transient gene expression
    Depositor
    Insert
    15xUAS::destabilizedYFP::let-838 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    YFP with destabilized signal from mouse ornithine…
    Promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pJR017

    Plasmid
    #198835
    Purpose
    In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.
    Depositor
    Insert
    15xUAS::TeTX-SL2-mKate::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

    Plasmid
    #209086
    Purpose
    Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression
    Depositor
    Inserts
    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    Use
    Tags
    AviTag
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    TRE4G and hSyn
    Available since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)

    Plasmid
    #209085
    Purpose
    Ngn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expression
    Depositor
    Inserts
    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    TRE4G and hSyn
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. TFORF2531

    Plasmid
    #142954
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Insert
    SOX9 (SOX9 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pJL046

    Plasmid
    #198807
    Purpose
    In vivo neuronal inhibition through histamine-gated chloride channel. Neurons expressing HisCl1 transgene are inhibited after addition of histamine.
    Depositor
    Insert
    15xUAS::HisCl1-SL2-GFP::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pHW581

    Plasmid
    #198818
    Purpose
    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics
    Depositor
    Insert
    15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pHW449

    Plasmid
    #198805
    Purpose
    Direct light-gated cation channel from Chlamydomonas reinhardtii that allows neuron deploarization through brief pulses of blue light.
    Depositor
    Insert
    15xUAS::hChR2(H134R)-YFP::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. pDO001

    Plasmid
    #198828
    Purpose
    Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production.
    Depositor
    Insert
    15xUAS::miniSOG-103L-VAMP2-mCherry::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pHW582

    Plasmid
    #198819
    Purpose
    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, higher baseline fluorescence
    Depositor
    Insert
    15xUAS::GCaMP7b-SL2-mKate2::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pHW539

    Plasmid
    #198811
    Purpose
    Dominant negative form of Protein Kinase A (PKA). Lowers PKA activity in targeted neurons.
    Depositor
    Insert
    15xUAS::PKA(DN)-SL2-GFP::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    G310D mutation to abolish the binding of cAMP of …
    Promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 351 - 400 of 471 results