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Plasmid#85584PurposecGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegansDepositorInsert15xUAS-delta pes-10-GFP
UseTagsExpressionWormMutationGFP(S65C) with three introns for expression in C.…Promoter15xUAS-delta pes-10-GFPAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJR019
Plasmid#198834PurposeInactivation of G protein subunits through Pertussis toxin (PTX)DepositorInsert15xUAS::PTX-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-FLNa S2152A
Plasmid#8983DepositorInsertFilamin A S2152A (FLNA Human)
UseTagsmycExpressionMammalianMutationSer2152AlaPromoterAvailable sinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-sh-hSOX9-1
Plasmid#40644DepositorInsertsh-hSOX9-1 (SOX9 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJR021
Plasmid#198814PurposeVariant of yellow fluorescent protein for transient gene expressionDepositorInsert15xUAS::destabilizedYFP::let-838 3'UTR
UseTagsExpressionWormMutationYFP with destabilized signal from mouse ornithine…PromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)
Plasmid#85583PurposecGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)DepositorInsertPrab-3-GAL4-SK(DBD)-VP64
UseTagsExpressionWormMutationPromoterPrab-3-GAL4-SK(DBD)-VP64Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLUT7 HA-GLI1
Plasmid#62970PurposeExpresses HA-tagged Gli1DepositorInsertGLI1 (GLI1 Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABEPuro-HA-GLI1
Plasmid#62967PurposeExpresses HA-tagged Gli1DepositorInsertGLI1 (GLI1 Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pNH11 (pmyo-2::Arch(D95N)::2xMycTag)
Plasmid#130275PurposeExpresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.DepositorInsertArch(D95N)
UseTags2x Myc-tagExpressionWormMutationPromoterAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21c(+)-mtPheRS
Plasmid#31080DepositorInsertMature form human mitochondrial phenylalanine-tRNA synthetase (FARS2 Human)
UseTagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Nt …PromoterT7Available sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNH13 (pmyo-2::QuasAr::mOrange)
Plasmid#130273PurposeExpresses the eFRET-based voltage sensor QuasAr::mOrange in the pharynx of C. elegans.DepositorInsertQuasAr::mOrange
UseTagsmOrangeExpressionWormMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNH12 (pmyo-2::MacQ::mCitrine)
Plasmid#130274PurposeExpresses the eFRET-based voltage sensor MacQ::mCitrine in the pharynx of C. elegans.DepositorInsertMacQ::mCitrine
UseTagsmCitrineExpressionWormMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GLI(-)TAD
Plasmid#37113DepositorInsertGLI1 (GLI1 Human)
UseTagsExpressionMammalianMutationdeleted 1,409 bp AccI fragment from 3' end o…PromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
625_HOXD13 in pmiRGlo
Plasmid#78129Purposeluciferase reporter for miRNA activityDepositorInsertHOXD13 (HOXD13 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation3'UTR containing miRNA binding sitesPromoterPGKAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA_HOXD13-mcherry
Plasmid#159793PurposeS. pyogenes sgRNA collocated with pegRNA targeting mouse HOXD13 geneDepositorInsertspacer of sgRNA targeting HOXD13 gene (HOXD13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJR017
Plasmid#198835PurposeIn vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.DepositorInsert15xUAS::TeTX-SL2-mKate::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR032
Plasmid#198773PurposeStabilized step function Opsins ChR2 variantDepositorInsert15xUAS::hChR2 (C128S, D156A)-EYFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR035
Plasmid#198821PurposeFor tissue specific RNA-seqDepositorInsert15xUAS::rpl-22HA-SL2-mKate::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW491
Plasmid#198808PurposeIn vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.DepositorInsert15xUAS::Tetx cDNA::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL049
Plasmid#198809PurposeIn vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity.DepositorInsert15xUAS::GCaMP6s-SL2-mKate2::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR031
Plasmid#198825PurposeStable step function ChR2 variant. Activated by bluelight, terminated by green light.DepositorInsert15xUAS::ChR2(C128S)-EYFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR034
Plasmid#198829PurposeStep-Waveform Inhibitory ChannelrhodopsinDepositorInsert15xUAS::SwiCHR-TS-EYFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR033
Plasmid#198822PurposeChloride-conducting channelrhodopsinDepositorInsert15xUAS::UAS-iC1-c2-TS-EYFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)
Plasmid#209086PurposeNgn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expressionDepositorUseTagsAviTagExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)
Plasmid#209085PurposeNgn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expressionDepositorUseTagsExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-SOX9
Plasmid#170703Purposedoxycycline-inducible overexpression of SOX9DepositorInsertSOX9 (SOX9 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Perox-mTurq2-Apollo-NADP+
Plasmid#193304PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the peroxisomeDepositorInsertG6PD (G6PD Human)
UseTagsmTurqoise2, peroxisomal targeting sequenceExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2531
Plasmid#142954PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX9 (SOX9 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR014
Plasmid#198813PurposeNatural light-gated anion channels. In vivo inhibition of neurons using blue light.DepositorInsert15xUAS::GtACR2::SL2::GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
SOX9-T2A-H2B-EGFP repair template
Plasmid#167972PurposeSOX9 reporter repair templateDepositorInsertSRY-Box Transcription Factor 9 (SOX9 Human)
UseCRISPR; Donor templateTagsH2B-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL046
Plasmid#198807PurposeIn vivo neuronal inhibition through histamine-gated chloride channel. Neurons expressing HisCl1 transgene are inhibited after addition of histamine.DepositorInsert15xUAS::HisCl1-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mito-mTurq2-Apollo-NADP+
Plasmid#193303PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the mitochondriaDepositorInsertG6PD (G6PD Human)
UseTagsmTurqoise2 and mitochondrial targeting sequenceExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cyto-mTurq2-Apollo-NADP+
Plasmid#193301PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the cytoplasmDepositorInsertG6PD (G6PD Human)
UseTagsmTurqoise2ExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW581
Plasmid#198818PurposeIn vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kineticsDepositorInsert15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW449
Plasmid#198805PurposeDirect light-gated cation channel from Chlamydomonas reinhardtii that allows neuron deploarization through brief pulses of blue light.DepositorInsert15xUAS::hChR2(H134R)-YFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T236A
Plasmid#111455PurposeTransient expression of Flag-tagged human Sox9 with T236A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT236A mutationPromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nuc-mTurq2-Apollo-NADP+
Plasmid#193302PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the nucleusDepositorInsertG6PD (G6PD Human)
UseTagsSV40 NLS and mTurqoise2ExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDO001
Plasmid#198828PurposeInhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production.DepositorInsert15xUAS::miniSOG-103L-VAMP2-mCherry::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T240A
Plasmid#111457PurposeTransient expression of Flag-tagged human Sox9 with T240A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT240APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-GLI1
Plasmid#79497PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human GLI1 CDSDepositorInsertGLI1 (GLI1 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T236A/T240A
Plasmid#111458PurposeTransient expression of Flag-tagged human Sox9 with T236A/T240A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT236A and T240APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG69 G6PD 3'UTR mut
Plasmid#12053DepositorInsertG6PD 3'UTR mut (G6PD Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-pegRNA_HOXD13_CtoT-EGFP
Plasmid#159792PurposeS. pyogenes pegRNA for C to T mutation at the HOXD13 site of mouse cells using prime editingDepositorInsertspacer of pegRNA_HOXD13_CtoT targeting HOXD13 gene (HOXD13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-pegRNA_HOXD13_CtoG-EGFP
Plasmid#159791PurposeS. pyogenes pegRNA for C to G mutation at the HOXD13 site of mouse cells using prime editingDepositorInsertspacer of pegRNA_HOXD13_CtoG targeting HOXD13 gene (HOXD13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
shRNA_circMTO1_2
Plasmid#215233PurposeSupression of shcircMTO1(2,RI,3)_2 expressionDepositorInsertcircMTO1 shRNA 2 (MTO1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
shRNA_MTO1_2
Plasmid#215213PurposeSupression of shMTO1_2 expressionDepositorInsertMTO1 shRNA 2 (MTO1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW582
Plasmid#198819PurposeIn vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, higher baseline fluorescenceDepositorInsert15xUAS::GCaMP7b-SL2-mKate2::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR012
Plasmid#198823PurposeNucleolus-transport-enhanced Cre protein. Recombination-mediated gene expression knockout.DepositorInsert15xUAS::nCRE-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW539
Plasmid#198811PurposeDominant negative form of Protein Kinase A (PKA). Lowers PKA activity in targeted neurons.DepositorInsert15xUAS::PKA(DN)-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationG310D mutation to abolish the binding of cAMP of …PromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only