Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 21,995 results for: ESC

Showing: 3161 - 3200 of 21995 results

  1. 1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid
    #159676
    Purpose
    Plasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.
    Depositor
    Omar Akbari
    Article
    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1
    Insert
    pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    159676-plasmid-map-sequence-id-314890

    Enlarge

  3. pAAV-UBC-S2-cAMPr

    Plasmid
    #160723
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S2-cAMPr
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160723-plasmid-map-sequence-id-315524

    Enlarge

  7. pAAV-UBC-S3-ExRaiAKAR

    Plasmid
    #160724
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S3-ExRaiAKAR
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160724-plasmid-map-sequence-id-315754

    Enlarge

  8. pAAV-UBC-S4-ExRaiCKAR

    Plasmid
    #160725
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S4-ExRaiCKAR
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160725-plasmid-map-sequence-id-315528

    Enlarge

  10. pAAV-UBC-S2-RAB_EKARev

    Plasmid
    #160726
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S2-RAB_EKARev
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160726-plasmid-map-sequence-id-315763

    Enlarge

  12. pGL

    Plasmid
    #119952
    Purpose
    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102
    Inserts
    gpps
    limS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminal truncation of 56 amino acid residues (…
    Promoter
    Available since
    Feb. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119952-plasmid-map-sequence-id-235949

    Enlarge

  13. pAAV-UBC-S3-RAB_EKARev

    Plasmid
    #160727
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S3-RAB_EKARev
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160727-plasmid-map-sequence-id-315764

    Enlarge

  15. pAAV-UBC-S1-jRCaMP1a

    Plasmid
    #160728
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S1-jRCaMP1a
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160728-plasmid-map-sequence-id-315534

    Enlarge

  16. 1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid
    #159677
    Purpose
    Plasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.
    Depositor
    Omar Akbari
    Article
    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1
    Insert
    [Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    159677-plasmid-map-sequence-id-314891

    Enlarge

  17. pAED2

    Plasmid
    #234599
    Purpose
    Fluorescent reporter of the SOS response
    Depositor
    Tanel Tenson
    Article
    Kaldalu et al NPJ Antimicrob Resist. 2025 Apr 11;3(1):
    Inserts
    mScarlet-I
    gfp-mut2
    Use
    Reporter
    Tags
    Expression
    Bacterial
    Mutation
    GFPmut2 was derived from avGFP with the following…
    Promoter
    Ptet+dnaK P1 and cda
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234599-plasmid-map-sequence-id-467741

    Enlarge

  20. pCHMP4B-NS3-Blue

    Plasmid
    #223534
    Purpose
    Mammalian expression of CHMP4B fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.
    Depositor
    Winfried Weissenhorn
    Article
    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1
    Insert
    CHMP4B (CHMP4B Human)
    Use
    Tags
    NS3 Hepatitis C protease and mTurquoise
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    223534-plasmid-map-sequence-id-443365

    Enlarge

  21. pCHMP4B-mut-NS3-Green

    Plasmid
    #223531
    Purpose
    Mammalian expression of CHMP4B fused to the hepatitis C virus protease NS3 through a short linker containing mutated NS3 cleavage site, along with a green fluorescent protein.
    Depositor
    Winfried Weissenhorn
    Article
    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1
    Insert
    CHMP4B (CHMP4B Human)
    Use
    Tags
    NS3 Hepatitis C protease and mNeonGreen
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    223531-plasmid-map-sequence-id-443356

    Enlarge

  22. pCHMP3-mut-NS3-Green

    Plasmid
    #223530
    Purpose
    Mammalian expression of CHMP3 fused to the hepatitis C virus protease NS3 through a short linker containing mutated NS3 cleavage site, along with a green fluorescent protein.
    Depositor
    Winfried Weissenhorn
    Article
    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1
    Insert
    CHMP3 (CHMP3 Human)
    Use
    Tags
    NS3 Hepatitis C protease and mNeonGreen
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    223530-plasmid-map-sequence-id-443352

    Enlarge

  23. pCHMP2A-mut-NS3-Green

    Plasmid
    #223529
    Purpose
    Mammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing a mutated NS3 cleavage site, along with a green fluorescent protein.
    Depositor
    Winfried Weissenhorn
    Article
    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1
    Insert
    CHMP2A (CHMP2A Human)
    Use
    Tags
    NS3 Hepatitis C protease and mNeonGreen
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    223529-plasmid-map-sequence-id-443350

    Enlarge

  34. rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)

    Plasmid
    #122450
    Purpose
    Expression of inositol 1,4,5-trisphosphate 3-kinase A
    Depositor
    Christophe Erneux
    Article
    Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.
    Insert
    inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
    Use
    Tags
    beta-galactosidase
    Expression
    Bacterial
    Mutation
    coding sequence from V66 till stop codon
    Promoter
    Available since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    122450-plasmid-map-sequence-id-237990

    Enlarge

  35. pBABE-MCS(extended)-Puro (empty vector)

    Plasmid
    #172603
    Purpose
    Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    The multiple cloning site (MCS) has been edited/e…
    Promoter
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172603-plasmid-map-sequence-id-340216

    Enlarge

Showing: 3161 - 3200 of 21995 results