We narrowed to 11,195 results for: ena

1. pMOD_B2518

   Plasmid

   #91075

   Purpose

   Module B, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TaU6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CAV1-K*R-HA

   Plasmid

   #27765

   Depositor

   Ari Helenius

   Article

   Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

   Insert

   caveolin-1, lysine null mutant (CAV1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   All lysines mutated to arginines (K*R)

   Promoter

   Available since

   Aug. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Dead Streptavidin-SpyTag

   Plasmid

   #59548

   Purpose

   pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Dead Streptavidin-SpyTag
   

   Use

   Tags

   SpyTag

   Expression

   Bacterial

   Mutation

   The construct lacks final K of SpyTag

   Promoter

   T7

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pJRH056

   Plasmid

   #171637

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-tdTomato
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLKO.1-sh-mSlug-4

   Plasmid

   #40648

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   sh-mSlug-4 (Snai2 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pMOD_C2906

   Plasmid

   #91093

   Purpose

   Module C, Promoter: FMV 34S, Gene: TREX2 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TREX2 (+ BaeI site for GT donor cloning) (Trex2 Mouse)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   FMV 34S

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJMP1187

   Plasmid

   #119256

   Purpose

   rfp "test" strain

   Depositor

   Jason Peters, Oren Rosenberg, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NLS-HA-2xMCP-KRAB

   Plasmid

   #126589

   Purpose

   Expresses MCP (MS2 Coat Protein) fusion to KRAB in mammalian cells, lentiviral backbone

   Depositor

   David Segal

   Article

   O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

   Insert

   2XMCP-KRAB
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C promoter

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMXs_NMNAT3-FLAG

   Plasmid

   #133261

   Purpose

   Express FLAG-tagged NMNAT3 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   NMNAT3 (NMNAT3 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIW447-CRISPR XYL2

    Plasmid

    #98906

    Purpose

    K. marxianus CRISPR Plasmid for XYL2 editing

    Depositor

    Ian Wheeldon

    Article

    Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

    Inserts

    Codon optimized Cas9
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SB-SRT-Puro

    Plasmid

    #154888

    Purpose

    Sleeping Beauty self-reporting transposon with puromycin reporter

    Depositor

    Rob Mitra, Joseph Dougherty

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    Sleeping beauty SRT
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28_Human_SAT1_Cterm_His

    Plasmid

    #220487

    Purpose

    heterologous expression of SSAT1 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    SSAT1 (SAT1 Human)
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28_Human_SAT1_Nterm_His

    Plasmid

    #220482

    Purpose

    heterologous expression of SSAT1 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    SSAT1 (SAT1 Human)
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRDB_156

    Plasmid

    #216090

    Purpose

    Cas9 [Sp] CRISPRa targeting CD274, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD274 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEY51

    Plasmid

    #191054

    Purpose

    mec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    mec-3 (mec-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTn7·X-nia

    Plasmid

    #122592

    Purpose

    Plasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    xylS/Pm->Nia
    

    Use

    Synthetic Biology; Tn7 genomic integration

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXspuro-GFP-NBR1-dLIR

    Plasmid

    #74204

    Purpose

    Retroviral expression of GFP-NBR1 with aa 727-738 deletion

    Depositor

    Jayanta Debnath

    Article

    Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

    Insert

    neighbor of BRCA1 gene 1 (NBR1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 727-738

    Promoter

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

18. PB-SRT-Puro

    Plasmid

    #154884

    Purpose

    piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

    Depositor

    Rob Mitra, Joseph Dougherty

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    piggyBac SRT
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

    Plasmid

    #170182

    Purpose

    Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    mCherry
    

    Use

    Tags

    3XFlag and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJMP1189

    Plasmid

    #119257

    Purpose

    rfp "test" strain

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEY52

    Plasmid

    #191055

    Purpose

    odr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    odr-1 (odr-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJMP1360

    Plasmid

    #119278

    Purpose

    for cloning new sgRNAs

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-U6sgbbBsmbI-puro-2A-Fluc

    Plasmid

    #100277

    Purpose

    Lentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imaging

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pETDuet RagA(D130N)-RagC

    Plasmid

    #99642

    Purpose

    Bacterial expression of RagA(D130N)-RagC

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D130N

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMA_Int

    Plasmid

    #110096

    Purpose

    E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Insert

    mycophage integrase
    

    Use

    Required for transformation of attb integrating v…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLKO.1-sh-mSlug-3

    Plasmid

    #40647

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSlug-3 (Snai2 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pETDuet RagA(T21N)-RagC

    Plasmid

    #99644

    Purpose

    Bacterial expression of RagA(T21N)-RagC

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    T21N

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AA009

    Plasmid

    #216037

    Purpose

    Fragmid fragment: (C' terminus) Nucleoplasmin NLS

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    NpNLS_v1.1; 3xHA_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AA345

    Plasmid

    #216031

    Purpose

    Fragmid fragment: (Cas protein) Cas13d

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas13d_v1.1 [Dj]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRDA_834

    Plasmid

    #216087

    Purpose

    Cas9 [Sp] knockout targeting CD59, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD59 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSicoR-TMF1i2

    Plasmid

    #59625

    Purpose

    Expression of shRNA against human TMF1

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    TMF1 (TMF1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pSicoR-TMF1i1

    Plasmid

    #59624

    Purpose

    Expression of shRNA against human TMF1

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    TMF1 (TMF1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pBEAST-pBen-Luc

    Plasmid

    #122694

    Purpose

    Output expression of firefly luciferase under the activation of BenR transcription factor for cell-free expression

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    Firefly luciferase
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGEX-4T-1-RIPK3

    Plasmid

    #78827

    Purpose

    To purify GST-tagged recombinant RIPK3 protein

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. cre_CD

    Plasmid

    #66030

    Purpose

    MoClo Basic Part: CDS - cre recombinase (recombines DNA at loxP sites) [C:cre:D]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pW211-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR

    Plasmid

    #170809

    Purpose

    Lentiviral vector to co-express an spsgRNA with NLS-mNeonGreen

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #170810

    Purpose

    Lentiviral vector to co-express an spsgRNA with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CAV1-mEGFP-mCherry

    Plasmid

    #27763

    Depositor

    Ari Helenius

    Article

    Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

    Insert

    caveolin-1 (CAV1 Canis lupus familiaris)
    

    Use

    Tags

    monomeric EGFP-monomeric Cherry (tandem)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBAD-Ag43

    Plasmid

    #107743

    Purpose

    Expresses Ag43 under the control of an L-arabinose transcriptional regulator

    Depositor

    Ingmar Riedel-Kruse

    Article

    Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

    Insert

    Ag43 (flu )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    removed all pstI cut sites with silent mutations,…

    Promoter

    pBAD

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGEX-parkin 141-465

    Plasmid

    #45971

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin 141-465 (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Dele…

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge