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173,361 results

Showing: 2491 - 2520 of 173361 results

  1. pLV-Golgi eGFP

    Plasmid
    #79809
    Purpose
    To express and target GFP into the Golgi apparatus using lentiviruses
    Depositor
    Pantelis Tsoulfas
    Insert
    Golgi eGFP (B4GALT1 Human)
    Use
    Lentiviral
    Tags
    Fusion of the first 62 amino acids of the human b…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    79809-plasmid-map-sequence-id-500311

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  6. pAAV-Ef1a-fDIO mCherry (AAV Retrograde)

    Viral Prep
    #114471-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Flp-dependent)
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only

  8. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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  10. pRTC2-puro

    Plasmid
    #190567
    Purpose
    Expresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.
    Depositor
    Anthony Furano
    Article
    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112
    Insert
    L1 (L1.3) with reporter cassette
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    190567-plasmid-map-sequence-id-393296

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  11. pKAR2-Br512

    Plasmid
    #161875
    Purpose
    Expresses Br512 polymerase in E.coli
    Depositor
    Andrew Ellington
    Article
    Maranhao et al medRxiv
    Insert
    Modified DNA Polymerase I Large Fragment (Br512)
    Tags
    8x His tag; Villin Headpiece 47 amino acid (HP47)…
    Expression
    Bacterial
    Promoter
    T7, tetPA
    Available Since
    Nov. 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    161875-plasmid-map-sequence-id-316881

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  16. PX458 V3

    Plasmid
    #226957
    Purpose
    CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226957-plasmid-map-sequence-id-450777

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  18. pcDNA3HA-AR-LBD

    Plasmid
    #89111
    Purpose
    mammalian expression of human androgen receptor ligand binding domain amino acid residues 624-919 with N-terminal double HA tag
    Depositor
    Elizabeth Wilson
    Article
    He et al J Biol Chem. 1999 Dec 24;274(52):37219-2
    Insert
    hAR ligand binding domain (AR Human)
    Tags
    2xHA
    Expression
    Mammalian
    Mutation
    contains Ligand Binding Domain (624-919)
    Promoter
    CMV
    Available Since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    89111-plasmid-map-sequence-id-176143

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  25. pGP-AAV-syn-jGCaMP8m-WPRE (AAV Retrograde)

    Viral Prep
    #162375-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP8m-WPRE (#162375). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8m-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available Since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only

  27. pLV-EF1A-CD19.8H.8TM.BB.28.3z

    Plasmid
    #200673
    Purpose
    Modular CD8hinge-CD8transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Michele Bernasconi
    Article
    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):
    Insert
    FMC63-CD8HD-CD8TM-41BB-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Promoter
    EF1a-short
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    200673-plasmid-map-sequence-id-391864

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Showing: 2491 - 2520 of 173361 results