167,910 results

1. pDONR223_CHD2_WT

   Plasmid

   #81768

   Purpose

   Gateway Donor vector containing CHD2 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CHD2 (CHD2 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn1-tdTomatof

   Plasmid

   #104060

   Purpose

   Also known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracing

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   tdTomato
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-SspB-mScarlet-I

   Plasmid

   #178521

   Purpose

   Expresses SspB-mScarlet-I in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   SspB-mScarlet-I
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE-hygro-2xMyc-Rac1-WT

   Plasmid

   #128580

   Purpose

   For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   2xMyc epitope

   Expression

   Mammalian

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-AAV-syn-jGCaMP8m-WPRE (AAV PHP.eB)

   Viral Prep

   #162375-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP8m-WPRE (#162375). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8m-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   Syn

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

6. pX330A_dCas9-1x4

   Plasmid

   #63598

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE

   Plasmid

   #123362

   Purpose

   Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Methods. 2019 May;16(5):405-408. doi

   Insert

   Firefly luciferase
   

   Use

   CRISPR, Lentiviral, and Luciferase

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE-hygro-2xMyc-Rac1-P29S

   Plasmid

   #128581

   Purpose

   For mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   2xMyc epitope

   Expression

   Mammalian

   Mutation

   Changed Proline29 to Serine.

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Plenti-CMV-MCS-RFP-SV-puro

   Plasmid

   #109377

   Purpose

   This plasmid expresses dsRed2 and puromycin resistance

   Depositor

   Jonathan Garlick, Behzad Gerami-Naini

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   dsRed2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. YEpADE1

    Plasmid

    #22495

    Depositor

    Beth Montelone

    Insert

    ADE1 (ADE1 Budding Yeast)
    

    Use

    Yeast shuttle

    Mutation

    Original 4067 bp HindIII-HindIII insertion (see Y…

    Available Since

    Jan. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSR62

    Plasmid

    #125076

    Purpose

    Expression of ompR under PLtetO-1, output promoter PompF146

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    ompR (ompR )
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEGFP-2xbiRhoBAST

    Plasmid

    #196349

    Purpose

    Expression of mEGFP mRNA with 2xbiRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

    Insert

    mEGFP-2xbiRhoBAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRES2-DsRed-MST-Kv2.1

    Plasmid

    #131707

    Purpose

    Expresses Kv2.1in mammalian cells

    Depositor

    Jennifer Kearney

    Article

    Jorge et al Proc Natl Acad Sci U S A. 2011 Mar 29;10

    Insert

    KCNB1 (KCNB1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. mEGFP-4xbiRhoBAST

    Plasmid

    #196350

    Purpose

    Expression of mEGFP mRNA with 4xbiRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

    Insert

    mEGFP-4xbiRhoBAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEE392

    Plasmid

    #149282

    Purpose

    T-DNA encoding TRV2 with mFT augmented gRNA targeting NbPDS3

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbPDS3sgRNA_mFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

16. psd44-G13WT

    Plasmid

    #46829

    Purpose

    expression of wild type G protein alpha 13 in mammalian cells

    Depositor

    Agnese Mariotti

    Insert

    GNA13 (GNA13 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK promoter

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

17. Anti-Kv2.1 K+ channel (External) [K39/25R]

    Plasmid

    #128620

    Purpose

    Mammalian Expression Plasmid of anti-Kv2.1 K+ channel (External) (Human). Derived from hybridoma K39/25.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Kv2.1 K+ Channel (External) (Human) recombinant mouse monoclonal antibody (KCNB1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-EGFP-C1-HsDCP2-E148Q_U

    Plasmid

    #147650

    Purpose

    Mammalian Expression of HsDCP2-E148Q

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsDCP2-E148Q (DCP2 Human)
    

    Expression

    Mammalian

    Mutation

    two silent mutations compared to the sequence giv…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRRL.SP146.gp91.eGFP

    Plasmid

    #30474

    Depositor

    Didier Trono

    Article

    Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

    Insert

    SP146/gp91/eGFP
    

    Use

    Lentiviral

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. GOLPH3 shRNA

    Plasmid

    #163860

    Purpose

    shRNA targeting the coding sequences of GOLPH3

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    golgi phosphoprotein 3 (GOLPH3 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGWB440

    Plasmid

    #74826

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. P4-PE

    Plasmid

    #211375

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    P4-PE
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. Anti-GABA(B)R2 [N81/37.1R]

    Plasmid

    #114544

    Purpose

    Mammalian Expression Plasmid of anti-GABA(B)R2 (Human). Derived from hybridoma N81/37.1.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GABA(B)R2 (Homo sapiens) recombinant mouse monoclonal antibody (GABBR2 Mouse)
    

    Expression

    Mammalian

    Promoter

    dual CMV

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pRFP

    Plasmid

    #26924

    Depositor

    Ron Weiss

    Article

    Basu et al Nature. 2005 Apr 28. 434(7037):1130-4.

    Insert

    LacI, HcRed
    

    Expression

    Bacterial

    Available Since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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25. pBABE-hygro-2xMyc-Rac1-Q61L

    Plasmid

    #128582

    Purpose

    For mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

    Depositor

    Julian Downward

    Article

    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

    Insert

    Rac1 (Rac1 Mouse)
    

    Use

    Retroviral

    Tags

    2xMyc epitope

    Expression

    Mammalian

    Mutation

    Changed Glutamine61 to Leucine.

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLV.SP146.gp91.eGFP.cHS4

    Plasmid

    #30476

    Depositor

    Didier Trono

    Article

    Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

    Insert

    SP146/gp91/eGFP/cHS4
    

    Use

    Lentiviral

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSLQ11434

    Plasmid

    #183625

    Purpose

    pHR-R(HRE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE

    Depositor

    Stanley Qi

    Article

    Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

    Insert

    Cas12 Guide (crGFP*)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET28a(CTLA4)

    Plasmid

    #177905

    Purpose

    Bacterial expression of his10-tagged human CTLA4 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    CTLA4 (aa 183-223 only) (CTLA4 Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pT7-EGFP-C1-HsNot7-D40AE42A_L

    Plasmid

    #146890

    Purpose

    Mammalian Expression of HsNot7-D40AE42A. Please note that this plasmid does not contain the T7 promoter.

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    HsNot7-D40AE42A (CNOT7 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. Anti-Nav1.2 Na+ channel [K69/33R]

    Plasmid

    #199396

    Purpose

    Mammalian expression plasmid of Anti-Nav1.2 Na+ channel [K69/33R] (Rat). Derived from hybridoma K69/33.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-Nav1.2 Na+ channel (Rat) recombinant mouse monoclonal antibody (Scn2a Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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