We narrowed to 23,605 results for: crispr

1. pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)

   Plasmid

   #113040

   Purpose

   AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   gRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

   Plasmid

   #113042

   Purpose

   AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRetro-UMI

   Plasmid

   #222686

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Mutation

   PGK without BbsI cutsite

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pR6K-GFP-CASTIF

   Plasmid

   #199653

   Purpose

   R6K plasmid encoding a type I-F CAST but no CRISPR array

   Depositor

   IIya Finkelstein

   Insert

   CAST I-F systems
   

   Expression

   Bacterial

   Promoter

   J23119 promoter

   Available Since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB_gRNA_S.pyogenes_scaffold_BB

   Plasmid

   #226429

   Purpose

   PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.

   Depositor

   Alon Goren

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-dCas9-VP64-BFP

   Plasmid

   #196711

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-VP64-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-UCOE-dCas9-BFP-VP64

   Plasmid

   #196717

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-TagBFP-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-dSa-VPR

   Plasmid

   #99651

   Purpose

   Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9

   Depositor

   George Church

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VP64-p65-RTA activator

   Mutation

   dead Cas9

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcU6.3_gRNA_c1

   Plasmid

   #221381

   Purpose

   Chicken-specific U6 sgRNA expression mini-vector with 10x capture sequence 1

   Depositor

   James Briscoe, Tatjana Sauka-Spengler

   Type

   Empty backbone

   Use

   CRISPR

   Promoter

   G. gallus U6.3

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYJ12-PB-U6-ND1-acti-gRNA1

    Plasmid

    #131056

    Purpose

    Activation of NeuroD1 via SAM system

    Depositor

    Xiaojun Lian

    Insert

    NeuroD1 activation gRNA1 (NEUROD1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYJ13-PB-U6-ND1-acti-gRNA2

    Plasmid

    #131057

    Purpose

    Activation of NeuroD1 via SAM system

    Depositor

    Xiaojun Lian

    Insert

    NeuroD1 activation gRNA2 (NEUROD1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYJ14-PB-U6-ND1-acti-gRNA3

    Plasmid

    #131058

    Purpose

    Activation of NeuroD1 via SAM system

    Depositor

    Xiaojun Lian

    Insert

    NeuroD1 activation gRNA3 (NEUROD1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. 1203AE-CMV-Cas7.1-7.2-H43A-mutant

    Plasmid

    #221449

    Purpose

    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, H to A mutation at amino acid 43 in Cas7.1

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1203AF-CMV-Cas7.1-7.2-Y55A-mutant

    Plasmid

    #221450

    Purpose

    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, Y to A mutation at amino acid 55 in Cas7.1

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1203AG-CMV-Cas7.1-7.2-N152A-mutant

    Plasmid

    #221451

    Purpose

    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, N to A mutation at amino acid 152 in Cas7.1

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOliSpec5'

    Plasmid

    #228551

    Purpose

    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 5' region of aadA
    

    Use

    CRISPR

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOliSpec3'

    Plasmid

    #228550

    Purpose

    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.

    Depositor

    Jeferson Gross

    Insert

    Truncated 3' region of aadA
    

    Use

    CRISPR

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpec5'

    Plasmid

    #228549

    Purpose

    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 5' region of aadA
    

    Use

    CRISPR

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpec3'

    Plasmid

    #228548

    Purpose

    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 3' region of aadA
    

    Use

    CRISPR

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pErCas12a EZ Clone

    Plasmid

    #132641

    Purpose

    Expresses ErCas12a and an sgRNA to target a region of interest

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    AAV and CRISPR

    Tags

    3x HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Q926R (Please see depositor comments) and BsaI si…

    Promoter

    CMV Promoter

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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