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pBS3Clux

Plasmid

#55172

Purpose

lux-reporter vector, integration at sacA, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCLucIPZ

Plasmid

#53222

Purpose

Constitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

Depositor

Thorsten Stiewe

Article

Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

Inserts

Cypridina luciferase
non-silencing shRNA

Use

Lentiviral

Expression

Mammalian

Available Since

June 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pJet1.2 ermB cassette

Plasmid

#117121

Purpose

Erythromycin resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Erythromycin resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJMP3

Plasmid

#79875

Purpose

Bacillus subtilis sgRNA expression vector; integrates into thrC

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

sgRNA RR1

Use

CRISPR

Expression

Bacterial

Promoter

veg

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas9_sgRNA_0

Plasmid

#70763

Purpose

expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating

Depositor

Regine Kahmann

Article

Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10

Inserts

U6 promoter
cas9
tnos terminator

Use

Self-replicating in ustilago maydis, conferring c…

Tags

N-terminal NLS, C-terminal HA-tag +NLS

Mutation

Streptococcus pyogenes gene codon-optimized for U…

Promoter

U6 from Ustilago maydis and synthetic otef promot…

Available Since

Nov. 12, 2015

Availability

Academic Institutions and Nonprofits only

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SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

Plasmid

#123615

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A/K34A)-P2A-EGFP

Expression

Mammalian

Mutation

R33A/K34A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBS4S

Plasmid

#55170

Purpose

Empty vector, integration at thrC, ampr, specr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pET28a-MH6-Bsu

Plasmid

#163911

Purpose

Expresses Bsu large fragment for affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

Bsu polymerase

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pC0037

Plasmid

#181934

Purpose

Dual secreted luciferase reporter (EF1alpha-Gaussia luciferase, CMV-Cypridina luciferase)

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Inserts

Gaussia luciferase
Cypridina luciferase

Expression

Mammalian

Promoter

CMV and EF1alpha

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCSCX

Plasmid

#63918

Purpose

Expression of the sucrose utilization phenotype

Depositor

Claudia Vickers

Article

Bruschi et al Biotechnol Adv. 2012 Sep-Oct;30(5):1001-

Insert

cscAKB

Use

Bacterial cloning vector

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pJQ200SK

Plasmid

#78497

Purpose

Suicide vector for gene replacement

Depositor

Michael Hynes

Article

Quandt et al Gene. 1993 May 15;127(1):15-21.

Type

Empty backbone

Use

Suicide plasmid

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5RGDPK7

Plasmid

#198291

Purpose

Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions)

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5RGDPK7

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRH030

Plasmid

#159633

Purpose

CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

Depositor

Robert Hertel

Article

Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

Type

Empty backbone

Use

CRISPR; Shuttle vector

Promoter

Constitutive tracrRNA

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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PxylA

Plasmid

#55178

Purpose

Xylose-inducible promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

xylA promoter

Use

Synthetic Biology; Bacillus biobrick box

Promoter

xylA

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

Plasmid

#123614

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A)-P2A-EGFP

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR/pSM2(CMV/TO) (w55-1)

Plasmid

#17388

Purpose

Gateway entry vector for miRNA expression under CMV/TO promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and RNAi

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pSIM24 KanR

Plasmid

#67583

Purpose

Plant Gene Expression Vector

Depositor

Indu Maiti

Article

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Type

Empty backbone

Expression

Plant

Promoter

M24

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pT2SK

Plasmid

#59383

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-kan

Use

Template

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-Cre

Plasmid

#198274

Purpose

For loading Cre into PVCs; also contains PVC regulatory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-Cre

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti X1 Puro empty (w610-1)

Plasmid

#20953

Purpose

3rd gen lentiviral empty control vector, Puro

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Neo DEST (w18-1)

Plasmid

#17390

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning, Lentiviral, and RNAi

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR4-GST 6P-1 (w487-1)

Plasmid

#17741

Purpose

Gateway entry vector for N-term GST fusion, frame 6P-1

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

GST

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

Plasmid

#74010

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5xUAS
Cas9
Cre
U6 promoter

Use

CRISPR

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR4-myc-nuc (w556-1)

Plasmid

#20026

Purpose

Gateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

Myc and NLS

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO V5-LUC Puro (w549-1)

Plasmid

#19785

Purpose

3rd gen lentiviral V5-Luciferase expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pMyNT-kan

Plasmid

#84693

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

Acetamidase

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEF-ENTR-LacZ (710-1)

Plasmid

#17430

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

LacZ

Use

Gateway Cloning

Tags

His and V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pUG6SP-tTA

Plasmid

#41029

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tTA (tetR-VP16)

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gαi*-BERKY3

Plasmid

#158198

Purpose

BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pH2B-miRFP670nano

Plasmid

#127438

Purpose

Histone 2B labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

H2B-miRFP670nano (H2BC11 Synthetic, Human)

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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