We narrowed to 12,912 results for: Pol

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poly E67R SUMO1

Plasmid

#221072

Purpose

Expression of poly E67R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of E67R SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Mutation

E67R

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35K SUMO1

Plasmid

#221070

Purpose

Expression of poly H35K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35K SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Mutation

H35K

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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poly H35R SUMO1

Plasmid

#221069

Purpose

Expression of poly H35R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of H35R SUMO1 each separated by (GGS)4 (SUMO1 Human)

Tags

His and MBP

Expression

Bacterial

Mutation

H35R

Promoter

tac

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-BiPOLES-mRuby3

Plasmid

#227780

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons fused to mRuby3

Depositor

Hillel Adesnik

Insert

BiPOLES-mRuby3

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGGF-bGH polyA-G

Plasmid

#218902

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

bGH polyA

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAcBAC3-POLY-pnRFP

Plasmid

#201680

Purpose

Boost the expression of pnRFP in mammalian cells (e.g., macrophage RAW 264.7 cells)

Depositor

Huiwang Ai

Article

Pang et al ACS Chem Biol. 2023 Jun 16;18(6):1388-13

Insert

813

Expression

Mammalian

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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RS314-U6(PolII-Lsm)

Plasmid

#200772

Purpose

Pol II expression of U6 Lsm

Depositor

Aaron Hoskins

Article

Lipinski et al RNA. 2022 Dec;28(12):1606-1620. doi: 10.

Insert

Gal-SNR6-II

Expression

Bacterial and Yeast

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCK005 ubi:mCerulean_ubb-polyA

Plasmid

#195979

Purpose

Expression construct

Depositor

Alexa Burger, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

ubi:mCerulean

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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TRE3GV-somBiPOLES-mCerulean

Plasmid

#192578

Purpose

AAV-vector for bidirectional optogenetic manipulation of tetTag-positive neurons

Depositor

Simon Wiegert

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

TRE3GV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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CaMKII-somBiPOLES-eGFP

Plasmid

#192583

Purpose

AAV-vector for bidirectional optogenetic manipulation of excitatory neurons

Depositor

Simon Wiegert

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

CaMKIIa(0.4)

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Pole4 KO sgRNA

Plasmid

#186935

Purpose

Pole4 KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Pole4 KO sgRNA (Pole4 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Pole3 KO sgRNA

Plasmid

#186934

Purpose

Pole3 KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Pole3 KO sgRNA (Pole3 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPole#2/Cre

Plasmid

#173610

Purpose

Expresses a Pole-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Pole (Pole Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPole#1/Cre

Plasmid

#173609

Purpose

Expresses a Pole-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Pole (Pole Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-POLBHR-eGFP

Plasmid

#176064

Purpose

Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

EGFP-PolB (POLB Human)

Tags

EGFP

Expression

Mammalian

Mutation

pUC with endogenous SapI mutated out, PAM mutatio…

Promoter

N/A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27Linear-2, PolyU(0)

Plasmid

#166994

Purpose

Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(0)

Expression

Mammalian

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1, PolyU(0)

Plasmid

#166992

Purpose

Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(0)

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLY, Linear-3, PolyU(1)

Plasmid

#166988

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLNYtRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(1)

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLY, Linear-3, PolyU(4)

Plasmid

#166989

Purpose

Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(4)

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLN, Linear-3, PolyU(4)

Plasmid

#166985

Purpose

Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(4)

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGST-pol1-N2-2A2

Plasmid

#160991

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Mutation

F58A/D62A

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-pol1-N1-2A2

Plasmid

#160990

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Mutation

F58A/D62A

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(1)

Plasmid

#159479

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(1)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(2)

Plasmid

#159480

Purpose

Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(2)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(3)

Plasmid

#159481

Purpose

Tests for the impact of 3 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(3)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pmiRGLO-POLR2E-213

Plasmid

#117356

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR POLR2E-213

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Murine PGK-1

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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YCe1889 HC_Kan_SV40-polyA_p11

Plasmid

#100678

Purpose

part designed to occupy position 11 of EMMA. Functional category: polyadenylation signal sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

SV40-polyA

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

Plasmid

#168290

Purpose

CRISPR-activator Suntag-P65-HSF1 (SPH)

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

dCas9, Suntag-P65-HSF1

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RLUC POLIRES FLUC

Plasmid

#45642

Purpose

Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

Depositor

Nahum Sonenberg

Article

Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

Insert

RLUC - IRES - FLUC

Use

Luciferase

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-FHC-POLQ

Plasmid

#64875

Purpose

lentiviral expression of human POLQ

Depositor

Richard Wood

Article

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-EF1-FHC-POLQ-DM

Plasmid

#64880

Purpose

lentiviral expression of human POLQ double mutant (in POL and HDL domains)

Depositor

Richard Wood

Article

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

K121M and D2330A,Y2331A

Promoter

EF1alpha

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pFUGW-Pol2-ffLuc2-eGFP

Plasmid

#71394

Purpose

Lentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoter

Depositor

Glenn Merlino

Article

Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

Insert

Pol2-ffLuc2-eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

Pol2 (mouse RNA polymerase II gene promtoer)

Available Since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

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HO-hisG-URA3-hisG-poly-HO

Plasmid

#51663

Purpose

yeast plasmid for integration of target sequence at HO locus, URA3 selection

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-RanWT-mRFP1-polyA

Plasmid

#59750

Purpose

Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran-mRFP1

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-H3.3-polyA

Plasmid

#59780

Purpose

Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

H3F3B (H3-3B Human)

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-flag-mKdm4d-polyA

Plasmid

#61553

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Article

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Kdm4d (Kdm4d Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV and T7

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus-tagged Apollo-NADP+

Plasmid

#71797

Purpose

Expresses Venus-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

Depositor

Jonathan Rocheleau

Article

Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

Insert

Apollo-NADP+

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-flag-mZscan4d-polyA

Plasmid

#61831

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Article

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Zscan4d (Zscan4d Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV and T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Renilla luciferase-Pol III

Plasmid

#37380

Depositor

Norbert Perrimon

Article

Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

Insert

PolIII-Renilla control reporter (RpIII128 Fly)

Use

Luciferase

Expression

Mammalian

Promoter

RNA PolIII 128 subunit (RpIII128)

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
Cerulean-tagged Apollo-NADP+

Plasmid

#71800

Purpose

Expresses Cerulean-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

Depositor

Jonathan Rocheleau

Article

Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

Insert

Apollo-NADP+

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 321 - 360 of 12912 results

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