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Showing: 4451 - 4500 of 11732 results

  2. pCYC1pr-GNSI (LEU)

    Plasmid
    #111450
    Purpose
    GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Available Since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    111450-plasmid-map-sequence-id-211605

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  6. pCEP4-HLA-c-myc-optiT1R3 ECD-MHC

    Plasmid
    #113961
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain fused to a canonical
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-563 fused to a transmembrane tether
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113961-plasmid-map-sequence-id-215291

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  7. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid
    #50483
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    YFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the YFP open…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pJL CusS NarX Chimera

    Plasmid
    #225652
    Purpose
    Expresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    CusS-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available Since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225652-plasmid-map-sequence-id-455039

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  10. pJL NrsS NarX Chimera

    Plasmid
    #225653
    Purpose
    Expresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    NrsS-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225653-plasmid-map-sequence-id-454026

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  11. pJL VanS NarX Chimera

    Plasmid
    #225655
    Purpose
    Expresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    VanS-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225655-plasmid-map-sequence-id-454023

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  12. pJL RssA NarX Chimera

    Plasmid
    #225654
    Purpose
    Expresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    RssA-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225654-plasmid-map-sequence-id-454025

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  17. pRS405 PEX14-VAC8-RFP

    Plasmid
    #166844
    Purpose
    Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554
    Insert
    VAC8 (VAC8, PEX14 Budding Yeast)
    Tags
    PEX14 transmembrane domain and RFP
    Expression
    Yeast
    Mutation
    PEX14 transmembrane domain
    Promoter
    VAC8 promoter
    Available Since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166844-plasmid-map-sequence-id-329332

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  18. pRS405 SEC66-VAC8-RFP

    Plasmid
    #166843
    Purpose
    Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554
    Insert
    VAC8 (VAC8, SEC66 Budding Yeast)
    Tags
    RFP and SEC66 transmembrane domain
    Expression
    Yeast
    Mutation
    SEC66 transmembrane domain
    Promoter
    VAC8 promoter
    Available Since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166843-plasmid-map-sequence-id-329331

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  22. pcDNA3-Lyso-PTEN(A4)-mCherry

    Plasmid
    #184054
    Purpose
    Lysosome-targeted constitutively active mutant of phosphatase and tensin homolog (PTEN); tagged with mCherry.
    Depositor
    Jin Zhang
    Article
    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202
    Insert
    Lyso-PTEN(A4)-mCherry (PTEN Human)
    Tags
    Lysosome-associated membrane protein 1 (LAMP1). a…
    Expression
    Mammalian
    Mutation
    Ser 380/385 and Thr 382/383 in PTEN mutated to Al…
    Promoter
    CMV
    Available Since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184054-plasmid-map-sequence-id-363239

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  24. pCAG-mTHC

    Plasmid
    #112620
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-mTFP1 (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Tags
    mTeal (mTFP1)
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112620-plasmid-map-sequence-id-226488

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  25. CelTOS-COMP-blac-flag-his

    Plasmid
    #111014
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    cell traversal protein for ookinetes and sporozoites (CelTOS) (PF3D7_1216600 )
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    111014-plasmid-map-sequence-id-224577

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  26. GRP94-COMP-blac-flag-his

    Plasmid
    #111008
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    endoplasmin, putative (GRP94) (PFL1070c Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111008-plasmid-map-sequence-id-224562

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  27. pZE21/UBP1/ClpS_V65I

    Plasmid
    #98567
    Purpose
    Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon
    Depositor
    George Church
    Article
    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11
    Inserts
    Truncated ubiquitin cleavase, codon optimized
    Mutated ATP-dependent Clp protease adapter protein
    Expression
    Bacterial
    Mutation
    Contains the V65I mutation that increases discrim…
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98567-plasmid-map-sequence-id-203054

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  29. pVHC

    Plasmid
    #112614
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Venus (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    No Promoter
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112614-plasmid-map-sequence-id-224545

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  30. MAEBL-COMP-blac-flag-his

    Plasmid
    #110963
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    merozoite adhesive erythrocytic binding protein (MAEBL) (PF3D7_1147800.1 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    110963-plasmid-map-sequence-id-223595

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  34. Anti-Kv1.2 K+ channel [K14/16R] (Recombinant Antibody)

    Antibody
    #180086-rAb
    Purpose
    Anti-Kv1.2 K+ channel (Human) recombinant mouse monoclonal antibody
    Depositor
    James Trimmer
    Article
    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1
    Recommended Applications
    Immunohistochemistry and Western Blot
    Reactivity
    Frog, Human, Mouse, Rat, and Zebrafish
    Source Species
    Mouse
    Isotype
    IgG2a
    Trial Size
    Available to purchase
    Available Since
    March 28, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by James Trimmer, UC Davis

  40. InterCatch-1xL

    Plasmid
    #186907
    Purpose
    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance gene
    Depositor
    Taekjip Ha, Allen Liu
    Article
    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100
    Insert
    TfR-(GGGGS)1-SpyCatcher003-sfCherry1-10
    Expression
    Mammalian
    Available Since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    186907-plasmid-map-sequence-id-369404

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Showing: 4451 - 4500 of 11732 results