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pRK5myc Rac1 N17

Plasmid

#15904

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Rac1 (RAC1 Human)

Tags

myc

Expression

Mammalian

Mutation

T17N

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRK5myc Rac1 L61

Plasmid

#15903

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Rac1 (RAC1 Human)

Tags

myc

Expression

Mammalian

Mutation

Q61L

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCHERRY3

Plasmid

#24659

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Expression

Bacterial

Available Since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-XRN2_sgRNA_1

Plasmid

#242895

Purpose

PiggyBac cargo vector with XRN2 sgRNA 1 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Murvin et al bioRxiv 2025.07.16.665154

Insert

XRN2 (Xrn2 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pKN3097

Plasmid

#113392

Purpose

Galactose-inducible Ty3 with extra PPT- used for random bar code library

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Insert

Ty3

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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Dazl-CPG-TV

Plasmid

#70144

Purpose

a targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyA

Depositor

Rudolf Jaenisch

Article

Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

Insert

5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL

Use

Mouse Targeting and Synthetic Biology

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1_Ec_rpoZ

Plasmid

#128837

Purpose

E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

Depositor

Seth Darst

Article

Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

Insert

rpoZ (rpoZ E. coli)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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GALT-APEX2

Plasmid

#215554

Purpose

Stable, inducible expression in Golgi

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

APEX2-NES-GalT-EGFP

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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JME4472

Plasmid

#129658

Purpose

URA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Article

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pGL2B -1538/+64

Plasmid

#24942

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pWALIUM-intron 3x UAS attB

Plasmid

#206360

Purpose

includes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequences

Depositor

Erdem Bangi

Article

Teague et al PLoS Genet. 2023 Jun 2;19(6):e1010792. d

Type

Empty backbone

Expression

Insect

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMV306G13+FFluc

Plasmid

#26157

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

G13 promoter + Firefly luciferase

Use

Mycobacteria integrating vector

Mutation

Codon optimized for Mycobacterium tuberculosis. …

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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LAMP1-APEX2

Plasmid

#215555

Purpose

Stable, inducible expression in lysosome

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

LAMP1-EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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B108-GFP-IRES-AP

Plasmid

#73988

Purpose

B108 enhancer of Blimp1 gene drives EGFP expression.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

B108 enhancer

Expression

Mammalian

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGEMT-PTE2A

Plasmid

#111773

Purpose

Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Type

Empty backbone

Use

Cloning intermediate

Promoter

T7

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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hMTP-flag

Plasmid

#138335

Purpose

Expresses MTP

Depositor

Mahmood Hussain

Article

Rava et al J Biol Chem. 2006 Apr 21;281(16):11019-2

Insert

MTTP (MTTP Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-eGFP-W3SL

Plasmid

#218742

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKM061

Plasmid

#134174

Purpose

Bacterial expression of GST-HJURP

Depositor

Iain Cheeseman

Article

McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

Insert

HJURP (HJURP Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMV306hsp+FFluc

Plasmid

#26156

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Firefly luciferase

Expression

Bacterial

Mutation

Firefly luciferase gene codon optimised for mycob…

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA-Tet1

Plasmid

#85742

Purpose

shRNA against mouse Tet1

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA Tet1

Use

AAV

Tags

EYFP

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pOPINS-UBE3C

Plasmid

#66711

Purpose

Expresses UBE3C HECT domain in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

KiAA0010 (UBE3C )

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
pmCherry-TPX2

Plasmid

#31227

Depositor

Patricia Wadsworth

Article

Ma et al Mol Biol Cell. 2010 Mar 15. 21(6):979-88

Insert

TPX2 (TPX2 Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-SENP2

Plasmid

#18047

Depositor

Edward Yeh

Article

Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
ELP-TEMP/pcDNA3.1(-)

Plasmid

#178434

Purpose

Genetically encoded ratiometric fluorescent thermometer

Depositor

Takeharu Nagai

Article

Vu et al Sci Rep. 2021 Aug 13;11(1):16519. doi: 1

Insert

ELP-TEMP

Tags

No tag

Expression

Mammalian

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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LifeAct-scramble

Plasmid

#217499

Purpose

Expresses LifeAct-mScarlet-scramble to serve as control plasmid for LifeAct-MTED

Depositor

Erik Dent

Article

Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

Insert

LifeAct-mScarlet-scramble

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyLigase

Plasmid

#51722

Purpose

Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

Depositor

Mark Howarth

Article

Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

Insert

SpyLigase

Tags

His6 Tag

Expression

Bacterial

Promoter

T7

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pCS6

Plasmid

#55752

Purpose

Produces T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

T7 RNA Polymerase

Expression

Bacterial

Promoter

pBAD

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGateway 3XFlag Prickle2 flag tagged

Plasmid

#24645

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

Prickle 2 (PRICKLE2 Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

April 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3 Tat HA (P#29)

Plasmid

#14654

Depositor

Matija Peterlin

Article

Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

Insert

Tat (tat HIV)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pENV8(WT)-GFPuv

Plasmid

#70127

Purpose

Cobalamin riboswitch reporter vector for use in E. coli

Depositor

Robert Batey

Article

Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

Insert

wild type environmental cobalamin riboswitch sequence (env8)

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

tac

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CTAG_mScarlet-I

Plasmid

#136118

Purpose

FP for C-term fusion with CDS12

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG-mScarletI(noATG)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBP-BBa_J23100

Plasmid

#74097

Purpose

BBa_J23100 promoter (iGEM collection)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_J23100 constitutive promoter (Sigma70)

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM4Z-Fluc

Plasmid

#183474

Purpose

This plasmid can be used to prepare Fluc mRNA by In Vitro Transcription

Depositor

Chantal Pichon

Article

Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

Insert

Firefly luciferase

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8379

Plasmid

#126913

Purpose

Plasmid containing integration cassette at X-4 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and XdCrtYB

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8380

Plasmid

#126914

Purpose

Plasmid containing integration cassette at XII-5 integration site in Saccharomyces cerevisiae for overexpression of XdCrtE

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtE

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4-PKCZeta WT His tagged

Plasmid

#24609

Depositor

Jeff Wrana

Article

Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

Insert

PKCZeta (PRKCZ Human)

Tags

His

Expression

Mammalian

Available Since

May 5, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-hSyn-ConVERGD-eGFP-WPRE

Plasmid

#218743

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-RRN3/TIF1A

Plasmid

#17661

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RRN3/TIF1A (RRN3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

See depositor comments below

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
IVT-MCP-Phi29

Plasmid

#207456

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

MCP-Phi29

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
stathmin-GFP

Plasmid

#86782

Purpose

Mammalian expression of stathmin-GFP

Depositor

Lynne Cassimeris

Article

Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

Insert

stathmin (STMN1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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