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We narrowed to 100,187 results for: Gene

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Showing: 2851 - 2880 of 100187 results

  6. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  8. T7-VEE-OKS-iM

    Plasmid
    #58972
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Promoter
    26S subgenomic promoter and IRES
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58972-plasmid-map-sequence-id-466890

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  21. pAAV-double floxed-eNpHR-EYFP-WPRE-pA

    Plasmid
    #20949
    Purpose
    Cre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to EYFP for optogenetic inhibition
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    halorhodopsin
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available Since
    May 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    20949-plasmid-map-sequence-id-200280

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  24. MSP2443

    Plasmid
    #72252
    Purpose
    Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72252-plasmid-map-sequence-id-207329

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  27. pDULE-ABK

    Plasmid
    #49086
    Purpose
    A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments
    Depositor
    Peter Schultz
    Article
    Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.
    Inserts
    tRNA synthetase
    tRNA for a photocrosslinking amino acid
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    GlnRS and lpp
    Available Since
    Nov. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    49086-plasmid-map-sequence-id-396431

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Showing: 2851 - 2880 of 100187 results