Showing: 1 - 20 of 64 results
-
Plasmid#166438PurposeExpress pGEX-6P-1 Toxoplasma gondii CBS-pair domainDepositorInsertToxoplasma gondii CBS-pair domain
UseTagsGSTExpressionBacterialMutationPromoterTac, LacI, AmpAvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Toxoplasma gondii (delta prenylation motif)
Plasmid#170789PurposeExpresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)DepositorInsertToxoplasma gondii PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lacIAvailabilityAcademic Institutions and Nonprofits only -
Lourido Toxoplasma CRISPR Library V1
Pooled Library#80636PurposePlasmid sgRNA library containing ten guides against each of the 8,158 predicted Toxoplasma gondii protein coding genes.DepositorAvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal.Sth.dCas9
Plasmid#171088PurposeCRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.DepositorInsertsToxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
DHFR-TSc3
UseToxoplasma gondii expressionTagsExpressionMutationNote: A S245F mutation is present but did not app…PromoterDHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalExpressionMutationPromoterTgTUB1 and Toxoplasma U6AvailabilityAcademic Institutions and Nonprofits only -
pBM019
Plasmid#128179PurposeConstruct for generating stable Cas9-expressing Toxoplasma gondiiDepositorInsertssgRNA#1
SpCas9
Chloramphenicol acetyltransferase
UseCRISPR; Toxoplasma gondii expressionTags3X FLAG and Nuclear Localization SignalExpressionMutationPromoterTUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…AvailabilityAcademic Institutions and Nonprofits only -
pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)
Plasmid#171089PurposeCRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.DepositorInsertsDecoy sgRNA
dCas9
Chloramphenicol acetyltransferase
UseCRISPR; Toxoplasma gondii expressionTags3X FLAG and Nuclear Localization SignalExpressionMutationD10A, H840APromoterTUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…AvailabilityAcademic Institutions and Nonprofits only -
pLinker-AID-3xHA-HXGPRT-LoxP
Plasmid#86553Purposeused for a TIR1-AID system with CRISPR tagging technology in Toxoplasma gondiiDepositorInsertLinker-AID-3xHA-HXGPRT 3'UTR
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGCaMP5-DHFR
Plasmid#78706PurposeGCaMP5 under the Toxoplasma SAG1 5' UTR. Encodes a DHFR selectable marker.DepositorInsertsGCaMP5
DHFR
UseTagsExpressionMutationPromoterToxoplasma SAG1 5' UTRAvailabilityAcademic Institutions and Nonprofits only -
pSAG1::CAS9-U6::sgUPRT
Plasmid#54467PurposeEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.DepositorInsertSAG1 5' UTR
UseCRISPR; Toxoplasma expressionTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLinker-AID-Ty-HXGPRT-LoxP
Plasmid#86667Purposefor generation of AID strains using CRISPR tagging technologyDepositorInsertLinker-AID-Ty-HGPRT 3'UTR
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLinker-AID-3xHA-DHFR-LoxP
Plasmid#86669Purposefor generation of AID strains using CRISPR tagging technologyDepositorInsertLinker-AID-3xHA-HXGPRT 3'UTR
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLoxP-DHFR-mCherry
Plasmid#70147PurposeExpresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletionDepositorInserta selectable dihydrofolate reductase-thymidylate synthase marker
UseExpression in toxoplasma gondiiTagsmCherryExpressionMutationPromoterDHFR 5' UTRAvailabilityAcademic Institutions and Nonprofits only -
pU6-DHFR
Plasmid#80329PurposeAllows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertDHFR-TSc3
UseCRISPRTagsExpressionMutationPromoterDHFR-TS 5' UTRAvailabilityAcademic Institutions and Nonprofits only -
pCAT-YFP-TetR
Plasmid#59018PurposeExpresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondiiDepositorInsertYFP-TetR
UseToxoplasma expressionTagsExpressionMutationPromoteralpha-tubulinAvailabilityAcademic Institutions and Nonprofits only -
pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP
Plasmid#171090PurposeCRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.DepositorInsertsSth1 dCas9
Chloramphenicol acetyltransferase
TagBFP
UseCRISPR; Toxoplasma gondii expressionTags3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…ExpressionMutationD9A, H599APromoterTUB1 (Toxoplasma gondii), transcriptionally linke…AvailabilityAcademic Institutions and Nonprofits only -
pLinker-6xHA-HXGPRT-LoxP
Plasmid#86552PurposeThe plasmid is used for endogenous tagging using CRISPR technology in ToxoplasmaDepositorInsertLinker-6xHA-HGPRT-LoxP
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGCaMP5
Plasmid#78705PurposeGCaMP5 under the Toxoplasma SAG1 5' UTR. No Toxoplasma selectable markerDepositorInsertGCaMP5
UseTagsExpressionMutationPromoterSAG1 5' UTRAvailabilityAcademic Institutions and Nonprofits only -
pLIC-SM-HA
Plasmid#111188PurposeHigh affinity HA Tagging of weakly expressed genes (optimized for Toxoplasma gondii)DepositorTypeEmpty backboneUseToxoplasma gondii expressionTagsSpaghetti Monster HA TagExpressionMutationPromoterEndogenousAvailabilityIndustry, Academic Institutions, and Nonprofits -
pLIC-SM-Myc
Plasmid#111189PurposeHigh affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)DepositorTypeEmpty backboneUseToxoplasma gondii expressionTagsSpaghetti Monster Myc TagExpressionMutationPromoterEndogenousAvailabilityIndustry, Academic Institutions, and Nonprofits
Showing: 1 - 20 of 64 results