161,273 results

1. pDE43-MCSq17

   Plasmid

   #27333

   Depositor

   Dirk Schnappinger

   Article

   Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

   Insert

   qtag17
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   Available since

   May 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mPiezo2-CMV-Sport6

   Plasmid

   #81073

   Purpose

   mouse Piezo2

   Depositor

   Ardem Patapoutian

   Article

   Coste et al Nat Commun. 2015 May 26;6:7223. doi: 10.

   Insert

   Piezo2 (please see depositor comments below) (Piezo2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJL1-CRM197-4xDQNAT

   Plasmid

   #128395

   Purpose

   In vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sites

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Diphtheria toxin
   

   Use

   Tags

   4xDQNAT glycosylation tag and 6xHis tag

   Expression

   Bacterial

   Mutation

   Inactivating G52E mutation

   Promoter

   T7

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMSCVII-mChe

   Plasmid

   #162753

   Purpose

   Retroviral (MSCV) expression of mCherry fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

   Insert

   mCherry
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMSCVII-E2Crimson

   Plasmid

   #162756

   Purpose

   Retroviral (MSCV) expression of E2-Crimson fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

   Insert

   E2-Crimson
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 5'-BirA-FLAG GFP minimal CMV pCDNA5

   Plasmid

   #212087

   Purpose

   LR vector for integration of GFP into N2a FRT rtTA3 expression cells (BioID vector)

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

   Plasmid

   #220612

   Purpose

   Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-ArgiNLS-AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Rtn4a-GFP

   Plasmid

   #61807

   Purpose

   Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

   Insert

   Rtn4a (RTN4 Human)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Rtn4a Stop Codon removed

   Promoter

   CMV

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSFFV_sfCherry3V(1-10)

   Plasmid

   #117483

   Purpose

   Lentiviral vector for constitutive expression of sfCherry3V(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   sfCherry3V(1-10)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   6 substitutions (K45R, G52D, L83W, T106A, K182R, …

   Promoter

   SFFV

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMSCVII-AM

    Plasmid

    #162751

    Purpose

    Retroviral (MSCV) expression of ametrine fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    ametrine
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA3 HaloTag-V5-TurboID

    Plasmid

    #226474

    Purpose

    Expresses fusion of HaloTag and TurboID in mammalian cells

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Cell Chem Biol. 2022 Dec 15;29(12):1739-

    Insert

    TurboID
    

    Use

    Tags

    HaloTag and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LeGO-E

    Plasmid

    #27359

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    U6 promoter, SFFV promoter, EmeraldGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLSG (stra8)

    Plasmid

    #21619

    Depositor

    David Garbers

    Article

    Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

    Insert

    Stra8 promoter (Stra8 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAS9counter

    Plasmid

    #107192

    Purpose

    Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

    Depositor

    Steve Salipante

    Article

    Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

    Inserts

    Cas9
    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    prab17

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV 3xFLAG-LC3C WT

    Plasmid

    #123095

    Purpose

    Expresses 3xFLAG-LC3C wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBAD miniSOG

    Plasmid

    #91774

    Purpose

    Bacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    mini Singlet Oxygen Generator
    

    Use

    Tags

    Hexa-Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-Clover-mRuby2

    Plasmid

    #49089

    Purpose

    Clover-mRuby2 tandem fusion for FRET calibration

    Depositor

    Kurt Beam

    Insert

    Clover-mRuby2 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO-PTEN-shRNA-3001

    Plasmid

    #25639

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    PTEN-shRNA-3001 (PTEN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

    Plasmid

    #122151

    Purpose

    Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    dCas9 fused to SunTag(24x)
    

    Use

    Lentiviral

    Tags

    2xNLS-P2A-BFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSCVII-LO

    Plasmid

    #162754

    Purpose

    Retroviral (MSCV) expression of LSSmOrange fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    LSSmOrange
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXSE901B

    Plasmid

    #91713

    Purpose

    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAW13.lentiguide.mCherry

    Plasmid

    #104375

    Purpose

    mCherry vector for cloning in guides

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSpCas9(BB)-2A-Neo

    Plasmid

    #127762

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MSCV-(mcs) human CSF1 full length-IRES-Puro

    Plasmid

    #86797

    Purpose

    retroviral expression of CSF-1

    Depositor

    Charles Sherr, Martine Roussel

    Article

    Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

    Insert

    CSF-1 (CSF1 Human)
    

    Use

    Retroviral

    Tags

    IRES-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRSETb-pcDronpa2-A69T

    Plasmid

    #98578

    Purpose

    photoconvertible fluorescent protein pcDronpa2-A69T in pRSetB plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

    Depositor

    Ulrike Endesfelder

    Article

    Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

    Insert

    pcDronpa2-A69T
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBA-mCherry-EGFP-PIM

    Plasmid

    #111758

    Purpose

    Low level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition

    Depositor

    Lukas Kapitein

    Article

    Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:

    Insert

    2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

    Promoter

    chicken beta-actin

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDRM18 LTN (Luciferase tdTomato Neo)

    Plasmid

    #174721

    Purpose

    Expresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    tdTomato
    aph
    

    Use

    Lentiviral and Luciferase

    Tags

    E2A linker and P2A linker

    Expression

    Mammalian

    Mutation

    Promoter

    MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pML107

    Plasmid

    #67639

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52 and pTDH3 (aka GAP promoter)

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PB_TetON_mCherry_P2AT2A_GFP-nb-KS-FtoG

    Plasmid

    #238242

    Purpose

    For integration of mCherry_P2AT2A_GFP-nb-KS-FtoG

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-KS-FtoG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDH-EF1-rtTA-PGK-BSD

    Plasmid

    #192885

    Purpose

    expression of rtTA

    Depositor

    Kazuhiro Oka

    Insert

    rtTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge