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167,150 results

Showing: 871 - 900 of 167150 results

  9. LSL-Cas9-Rosa26TV

    Plasmid
    #61408
    Purpose
    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    Cas9
    EGFP
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    3xFLAG and P2A
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    CAG
    Available Since
    Jan. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    61408-plasmid-map-sequence-id-333613

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  13. pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64

    Plasmid
    #203348
    Purpose
    Template for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Christopher Grigsby, Molly Stevens
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Promoter
    T7
    Available Since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203348-plasmid-map-sequence-id-399143

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  15. RNA-Protein Landing Pad

    Plasmid
    #226162
    Purpose
    Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression
    Depositor
    Dustin Maly
    Article
    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.
    Inserts
    mTagBFP2-P2A-iCasp9
    Reverse tetracycline transactivator 3
    Hygromycin-IRES-EGFP
    Use
    Synthetic Biology
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    226162-plasmid-map-sequence-id-450826

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  18. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid
    #139999
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139999-plasmid-map-sequence-id-346119

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  27. MBP-Benzonase

    Plasmid
    #218192
    Purpose
    Bacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001
    Depositor
    Cheryl Arrowsmith
    Article
    Rieubon et al protocols.io 2025
    Insert
    nucA
    Tags
    MBP
    Expression
    Bacterial
    Available Since
    April 24, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    218192-plasmid-map-sequence-id-431003

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  29. pcDNA3.1-Flag

    Plasmid
    #208051
    Purpose
    Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208051-plasmid-map-sequence-id-418310

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Showing: 871 - 900 of 167150 results