159,332 results

1. pT22-GluCNZ-6His

   Plasmid

   #213504

   Purpose

   Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

   Depositor

   John Denu

   Article

   Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

   Insert

   Glucanase (glucan endo-1,3-beta-glucosidase)
   

   Use

   Tags

   6His tag

   Expression

   Bacterial

   Mutation

   1-36 Signal Sequence Deletion

   Promoter

   T7

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV0286

   Plasmid

   #149333

   Purpose

   Bacterial expression of SENP_EuB protease

   Depositor

   Dirk Görlich

   Article

   Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

   Insert

   SENP_EuB protease
   

   Use

   Tags

   His14 and Tev protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDY1314_RADARS_Luciferase

   Plasmid

   #193191

   Purpose

   Express Gaussia Luciferase conditional upon RNA transcript

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

   Insert

   RADARS Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28b-Cas9-His

   Plasmid

   #47327

   Purpose

   For in vitro expression and purification of Cas9 protein

   Depositor

   Alex Schier

   Article

   Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSPgRNA

   Plasmid

   #47108

   Purpose

   Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Has Service

   Cloning Grade DNA

   Insert

   SPgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET22b - PA

   Plasmid

   #84863

   Purpose

   bacterial expression of Bacillus anthracis protective antigen

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Insert

   PA
   

   Use

   Tags

   pelB signal sequence

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH2B_mCherry_IRES_puro2

   Plasmid

   #21045

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   pH2B (H2BC16P Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tg(mpeg1:LifeAct-mRuby)

   Plasmid

   #166977

   Purpose

   labels F-actin in macrophages

   Depositor

   Anna Huttenlocher

   Article

   Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-380

   Inserts

   mpeg1 (mpeg1.1 Zebrafish)
   LifeAct (actb1 Frog)
   

   Use

   Tags

   Lifeact-mRuby and mRuby

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3puro-mGFP-FMR1 isoform1-sh1Resistant

   Plasmid

   #225462

   Purpose

   Express mEGFP-fusion protein of FMR1 isoform 1

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FMR1 isoform 1 (FMR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX333

    Plasmid

    #64073

    Purpose

    Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    3XFLAG-Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CBh; U6

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.CMV.SV40.THBS1-HA.SV40(polyA)

    Plasmid

    #154850

    Purpose

    Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 GFP LIC cloning vector (6D)

    Plasmid

    #30127

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. SPLICS Mt-ER Short P2A

    Plasmid

    #164108

    Purpose

    Detect the short Mitochondria-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Mt-ER Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFB2A

    Plasmid

    #195283

    Purpose

    Bicistronic DTR and Hygromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    DTR 2A Hygro (HBEGF Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CW3SL-EGFP

    Plasmid

    #61463

    Purpose

    AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK172-6xHis-0N4R-P301S-tau-HiBiT

    Plasmid

    #187835

    Purpose

    Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

    Depositor

    William McEwan

    Article

    Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

    Insert

    6XHis-0N4R-P301S-tau-HiBiT
    

    Use

    Tags

    6xHistidine and HiBiT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209652

    Purpose

    Expression of iATPSnFR2, medium affinity

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95K.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95K

    Promoter

    CAG

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBJ FLAG-hPar1

    Plasmid

    #53226

    Purpose

    mammalian expression of human Par1 with N-terminal FLAG tag

    Depositor

    Shaun Coughlin

    Article

    Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

    Insert

    Par1 (F2R Human)
    

    Use

    Tags

    FLAG and bovine prolactin signal sequence (MDSKGS…

    Expression

    Mammalian

    Mutation

    Deletion of aa1-39 (native signal peptide)

    Promoter

    SV40

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-U6-sgRNA(CTRL)-CMV-eGFP

    Plasmid

    #194017

    Purpose

    Expresses a gRNA that targets the LacZ gene (serves as control) and eGFP

    Depositor

    Larry Young

    Article

    Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

    Inserts

    sgRNA(CTRL)
    eGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)IRES GFP

    Plasmid

    #51406

    Purpose

    Expresses GFP from internal ribosome entry sequence, cloning component

    Depositor

    Kathleen_L Collins

    Article

    Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

    Insert

    IRES GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMito-iRFP670

    Plasmid

    #45462

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Use

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TFORF1207

    Plasmid

    #144089

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CASZ1 (CASZ1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HRV-B3 IRES

    Plasmid

    #189969

    Purpose

    Part 2 plasmid to introduce the HRV-B3 IRES into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    HRV-B3 IRES
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. alpha7nACHR_Nacho_GCAMP7s

    Plasmid

    #182940

    Purpose

    alpha7 nACHR expression in mammalian cell lines with calcium reporter

    Depositor

    Robert Brenner

    Article

    Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

    Insert

    NACH7 (CHRNA7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CXCR3-DuET

    Plasmid

    #213220

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR3 (CXCR3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-CMV-LoxP-DsRed-LoxP-eGFP

    Plasmid

    #65726

    Purpose

    Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

    Depositor

    Jacco van Rheenen

    Article

    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

    Insert

    LoxP-DsRed-LoxP-eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcDNA3 mTSP2

    Plasmid

    #12411

    Depositor

    Paul Bornstein

    Insert

    TSP2 (Thbs2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pHRm-GFP(1-10)-VP64-NLS

    Plasmid

    #70228

    Purpose

    Expresses GFP(1-10)-VP64-NLS in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP(1-10)-VP64-NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUltra-chPheRS

    Plasmid

    #182257

    Purpose

    Genetic Incorporation of DOPA

    Depositor

    Han Xiao

    Article

    Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

    Inserts

    chPheRS2 for DOPA
    chPheT for DOPA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-hSyn-RFP

    Plasmid

    #22907

    Depositor

    Edward Callaway

    Article

    Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

    Insert

    human Synapsin I promoter driving DsRedExpress (SYN1 Human)
    

    Use

    AAV; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge