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27,006 results

Showing: 5961 - 5980 of 27006 results

  6. mScarletI3-6DG5-MAPPER

    Plasmid
    #222421
    Purpose
    To express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)
    Depositor
    Stephen Royle
    Publication
    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAPPER
    Tags
    mScarletI3-6DG5
    Expression
    Mammalian
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222421-plasmid-map-sequence-id-439142

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  7. 3xFlag-eGFP-Flag-SETX

    Plasmid
    #218838
    Purpose
    Mammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.
    Depositor
    Albert La Spada
    Publication
    Bennett et al Heliyon. 2020 Jun 13;6(6):e04165. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Senataxin (SETX Human)
    Tags
    Flag and eGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    218838-plasmid-map-sequence-id-435865

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  8. ER-GA

    Plasmid
    #209871
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
    Depositor
    Sarah Cohen
    Publication
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddGFP A
    Tags
    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209871-plasmid-map-sequence-id-415464

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  10. pH2B-mNG2(11)

    Plasmid
    #206043
    Purpose
    Plasmid expressing a H2B-mNG2(11) fusion in mammalian cells for complementation with the mNG2(1-10) fragment.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B gene fused to the mNG2(11) fragment
    Use
    Synthetic Biology
    Tags
    H2B-mNG2(11)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    206043-plasmid-map-sequence-id-411120

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  12. TRUPATH Triple GaoA

    Plasmid
    #196051
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    196051-plasmid-map-sequence-id-385865

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  13. TRUPATH Triple Gai2

    Plasmid
    #196049
    Purpose
    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    196049-plasmid-map-sequence-id-385863

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  14. pcDNA3.1-LSSmGFP

    Plasmid
    #186543
    Purpose
    LSSmGFP fluorescent protein (ex/em = 400/510 nm): cytosolic expression in mammalian cells
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LSSmGFP
    Expression
    Mammalian
    Mutation
    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186543-plasmid-map-sequence-id-368052

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  18. pFX-mito-EGFP

    Plasmid
    #159096
    Purpose
    Express EGFP with mitochondria-targeting sequence in mammalian cells
    Depositor
    Yusuke Ohba
    Publication
    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry with mitochondria-targeting sequence
    Tags
    the mitochondria-targeting sequence of the cytoch…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    159096-plasmid-map-sequence-id-354796

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  19. pαH-S-GSAS-2P-OMICRON

    Plasmid
    #180593
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180593-plasmid-map-sequence-id-352871

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Showing: 5961 - 5980 of 27006 results