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30,870 results

Showing: 1581 - 1600 of 30870 results

  1. pcDNA3.1-P25C-GFP

    Plasmid
    #1343
    Depositor
    Li-Huei Tsai
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    P25 (CDK5R1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Available since
    April 13, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1343-plasmid-map-sequence-id-194980

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  2. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid
    #139996
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139996-plasmid-map-sequence-id-275405

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  3. pCMV-15F11-HA-Halo

    Plasmid
    #129592
    Purpose
    Encodes anti-HA scFv (anti-HA frankenbody) fused with HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Publication
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    129592-plasmid-map-sequence-id-254268

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  4. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid
    #107942
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R, and N282A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107942-plasmid-map-sequence-id-259760

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  5. pC035 - dLwCas13a-msfGFP

    Plasmid
    #91925
    Purpose
    Expresses catalytically inactive LwCas13a for mammalian RNA targeting
    Depositor
    Feng Zhang
    Publication
    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLwCas13a
    Use
    CRISPR
    Tags
    NLS-3xHA and NLS-msfGFP
    Expression
    Mammalian
    Mutation
    R488A and R1060A
    Promoter
    EF-1a
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91925-plasmid-map-sequence-id-228361

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  8. pEGFP-C1 Utr230-EGFP-3XNLS

    Plasmid
    #58466
    Purpose
    Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter
    Depositor
    Dyche Mullins
    Publication
    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Utr230 with 3X glycine linker (UTRN Human)
    3X NLS with 3X stop codons
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58466-plasmid-map-sequence-id-410899

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  9. pcDNA3-CaMPARI W391F V398L

    Plasmid
    #60787
    Purpose
    Expresses CaMPARI W391F V398L in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator
    Depositor
    Loren Looger, Eric Schreiter
    Publication
    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CaMPARI W391F V398L
    Tags
    Nuclear Export Signal
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    60787-plasmid-map-sequence-id-198297

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  12. M-NMn-VP64

    Plasmid
    #48676
    Purpose
    Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-VP64, nuclease-null
    Use
    CRISPR
    Tags
    NLS and VP64
    Mutation
    nuclease-null
    Promoter
    CMV
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48676-plasmid-map-sequence-id-71851

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  18. mTLR4

    Plasmid
    #13085
    Depositor
    Ruslan Medzhitov
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TLR4 (Tlr4 Mouse)
    Expression
    Mammalian
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13085-plasmid-map-sequence-id-142564

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  20. pAAV2/2

    Plasmid
    #232199
    Purpose
    AAV packaging plasmid expressing AAV2 Rep and capsid proteins
    Depositor
    Melina Fan, Addgene Research Program
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rep2/Cap2
    Use
    AAV
    Expression
    Mammalian
    Available since
    Sept. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    232199-plasmid-map-sequence-id-457007

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Showing: 1581 - 1600 of 30870 results