32,069 results
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pDSG375
Plasmid#115603Purposesurface-displayed Nb2; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-DAXX-His
Plasmid#175782PurposeDAXX protein for bacteria expression systemDepositorAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II SK--POLQ
Plasmid#64873Purposecloning vector for human POLQDepositorInsertPOLQ (POLQ Human)
ExpressionBacterialAvailable SinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
His6_BcLOV4_mCherry_BamUK
Plasmid#114596PurposeEncodes mCherry-tagged BcLOV4 for expression in bacterial cellsDepositorInsertBcLOV4
TagsHis6 and mCherryExpressionBacterialPromoterT7Available SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJLG029
Plasmid#192980PurposeBHR plasmid (pRA301) expressing pruR-3XFLAG from T7 promoter for high yield protein purificationDepositorInsertpruR-3xFLAG
Tags3XFLAGExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP3665
Plasmid#222351PurposeExpression vector with PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCT02
Plasmid#212152PurposePlasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.DepositorInsertFAST-PETase
Tags6x HisExpressionBacterialMutationS121E, D186H, R224Q, N233K, R280APromoterPT7Available SinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPgiCas13b
Plasmid#184833PurposeInducible expression of PgiCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Porphyromonas gingivalis
UseCRISPRExpressionBacterialPromoterT7 promoterAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS TIR-1 sfGFP
Plasmid#154463PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOSV00170
Plasmid#222934PurposeConstitutive GFP expression for Bacillus subtilisDepositorInsertGFP(Sp)
UseSynthetic BiologyExpressionBacterialPromoterPhyperspankAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30a-GlmU-NahK-ET64
Plasmid#234663PurposeExpression an ELP-tagged fusion enzyme of GlmU and NahKDepositorInsertsN-acetylglucosamine- 1-phosphate uridyltransferase
N-acetylhexosamine 1-kinase
TagsELPExpressionBacterialAvailable SinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHimar6
Plasmid#137081Purposebacterial Himar1 mini-transposon donor plasmidDepositorInsertHimar1 mini-transposon with chloramphenicol resistance gene
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISDge10_reRNA
Plasmid#205340PurposeBacterial expression of ISDge10 TnpB and its reRNADepositorInsertISDge10 TnpB
ExpressionBacterialAvailable SinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Olig2
Plasmid#64842PurposeA lentiviral vector for the expression of mouse Olig2 under doxycyline controlDepositorAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet3-H
Plasmid#242970PurposeDual expression vector for bacteria and mammalian cells producing mScarlet-3-H red fluorescent proteinDepositorInsertmScarlet3-H
ExpressionBacterial and MammalianPromoterCMV & RhaAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDF_AaRaf1/Raf2/RbcX2/BSD2/RbcX1
Plasmid#229509PurposeExpresses Anthoceros agrestis chaperones: Raf1,Raf2,RbcX2,BSD2,RbcX1DepositorInsertsRubisco accumulation factor 1
Rubisco accumulation factor 2
RbcX2
BSD2
RbcX1
ExpressionBacterialMutationN terminus chloroplast transit peptide truncationPromoterT7Available SinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSUMO mouse METAP1
Plasmid#197395PurposeExpresses SUMO-tagged mouse METAP1DepositorAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKnockinDonor-human-p16INK4A-P2A-mCherry
Plasmid#137026Purposedonor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4ADepositorInsertp16INK4A-knockin-homology arm-P2A-mCherry
TagsP2A-mCherryExpressionBacterialPromoterno promoterAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Hhe_Cas13a_WT
Plasmid#91871PurposeBacterial expression for Cas13aDepositorInsertHheCas13a (HheC2c2) WT
TagsHis6-MBP-TEV siteExpressionBacterialAvailable SinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBx-Spas-sgRNA-Gm
Plasmid#105232PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosaDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET41b+_Ufd1-His
Plasmid#117107PurposeProduction of recombinant Ufd1-His in E.coliDepositorAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG031 (upx)
Plasmid#157909PurposeExpresses the upx defense systemDepositorInsertupx (putative PD-(D/E)xK family phosphodiesterase)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGR596
Plasmid#213787PurposeIntegrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyExpressionBacterial and YeastMutationSee manuscriptAvailable SinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLG027 (qatABCD)
Plasmid#157905PurposeExpresses the qatABCD defense systemDepositorInsertqatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDawn-Ag43
Plasmid#107742PurposeExpresses Ag43 under the control of a blue-light transcriptional regulatorDepositorInsertAg43 (flu )
ExpressionBacterialMutationremoved all pstI cut sites with silent mutations,…PromoterpLambdaAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆FHA-mNeonGreen-IRESpuro2
Plasmid#183739PurposeExpresses Ki67∆FHA-mNeonGreenDepositorInsertMKI67_lacking FHA (MKI67 Human)
TagsmNeonGreenExpressionBacterial and MammalianMutationMKI67(lacking FHA)PromoterCMVAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSETa_ mEos4b
Plasmid#51073PurposePhotoconverible Fluorescent Protein_ Fixative Resistant (monomeric)DepositorInsertmEos4b
ExpressionBacterialPromoterT7Available SinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
AURKB
Plasmid#39119PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGGB_RT_Tq2CFP_li
Plasmid#136965PurposeGoldengate/Greengate N-tag module, containing Tq2CFP fused to a flexible linkerDepositorInsertTq2CFP
ExpressionBacterialAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB233
Plasmid#164128PurposePP7-CFP and PhlF-RFP expression plasmidDepositorInsertPP7-CFP
ExpressionBacterialPromoterPtetAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
proinsulin-EYFP
Plasmid#218952PurposeExpresses proinsulin fused to EYFP in bacteriaDepositorInsertproinsulin-EYFP
TagsproinsulinExpressionBacterialPromoterT5Available SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK603
Plasmid#8831DepositorInsertTEV protease, S219D mutant
ExpressionBacterialMutationS219D mutantAvailable SinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHP_YEAST
Plasmid#204233PurposeBacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHP_YEAST (MDH3 Budding Yeast)
Tags6X HisExpressionBacterialMutationThe last 3 C-terminal residues (SKL) are the pero…PromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
28A_APEX2_MSP1D1
Plasmid#170923PurposeExpress APEX2_MSP1D1 in Bl21DepositorInsertAPEX2-MSP
ExpressionBacterialAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWUR703
Plasmid#53082Purposeinducible TtAgoDM (D478A,D546A) expressionDepositorInsertTtAgo
TagsStrep-II tagExpressionBacterialMutationD478A D546APromoterT7Available SinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-NLS-6xHis
Plasmid#207379PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060APromoterT7Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-Cre-HiBiT
Plasmid#198318PurposeLoads HiBiT-tagged Cre into PVCs using Pdp1 packaging domain; also contains PnfDepositorInsertPdp1_NTD-Cre-HiBiT
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>
Plasmid#72240PurposeHistidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)DepositorInsertGLT1 GAL1p 24xPP7 V3
ExpressionBacterialAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTL032
Plasmid#80928Purpose14x MS2 sense + 14x PP7 antisense with loxP-kanMX-loxPDepositorInsert14xMS2 sense + 14xPP7 antisense, loxP-kanMX-loxP
ExpressionBacterialAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only
