29,712 results

1. pAG001 His6-TwinStrep-SUMO-AapCas12b

   Plasmid

   #153162

   Purpose

   Bacterial expression plasmid for AapCas12b

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

   Insert

   AapCas12b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINS-USP21 (USP, aa 196-565)

   Plasmid

   #61585

   Purpose

   Expresses human USP21 (USP domain) in E. coli.

   Depositor

   David Komander

   Article

   Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

   Insert

   USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   Deleted aa 1-195.

   Promoter

   T7

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST17-UbE2S

   Plasmid

   #15807

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2S (UBE2S Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKP112 (pRS406-GFP-ADH1t)

   Plasmid

   #106472

   Purpose

   Vector for creating C-terminal GFP fusion proteins in yeast.

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Type

   Empty backbone

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   None

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. WDR5 (2H9N)

   Plasmid

   #25229

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   WDR5_01 (WDR5 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pGEX-6P-1-hs-ALPS 1-ALPS2-EGFP

   Plasmid

   #187113

   Purpose

   Protein Expression

   Depositor

   Philipp Niethammer

   Article

   Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

   Insert

   ENSG00000101199 (ARFGAP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HC-M

   Plasmid

   #173481

   Purpose

   An rrnB P1-based GFP ATP reporter in E. coli

   Depositor

   Rahul Sarpeshkar

   Article

   Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

   Insert

   GFP-mut2
   

   Use

   Synthetic Biology

   Tags

   ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   rrnB P1

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSC101-AmpR EV

   Plasmid

   #208184

   Purpose

   empty plasmid vector, non-fluorescent

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Type

   Empty backbone

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-scFv-T

   Plasmid

   #67843

   Purpose

   pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

   Depositor

   Joanna Bereta

   Article

   Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

   Insert

   antibody 1D8 light kappa chain
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21b-Tipin-His

    Plasmid

    #23122

    Depositor

    Aziz Sancar

    Article

    Kemp et al J Biol Chem. 2010 Mar 15. ():.

    Insert

    Tipin (TIPIN Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

11. ETV5_pet28a

    Plasmid

    #131639

    Purpose

    Bacterial expression of ETS gene ETV5

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV5 (ETV5 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PP1 7-300

    Plasmid

    #26566

    Depositor

    Wolfgang Peti

    Article

    Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

    Insert

    PP1a (PPP1CA Human)
    

    Use

    Tags

    His6, TEV cleavage site, and Thio-6

    Expression

    Bacterial

    Mutation

    Contains only aa 7-330

    Promoter

    Available since

    Dec. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pGal4x5-E4T-G210

    Plasmid

    #171077

    Purpose

    The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    Gal4-E4-Gless-cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 4A3.PBAD-repL(WT).J23115-gfp

    Plasmid

    #197872

    Purpose

    Arabinose induced expression of wildtype repL gene, which triggers DNA replication in cis and in trans, weak level of constitutive GFP expression

    Depositor

    Baojun Wang

    Article

    Huan et al Front Microbiol. 2023 Feb 9;14:1095671.

    Inserts

    part kilA/repL
    gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    contains only the 24 bp of 3'-end of kilA ge…

    Promoter

    Bba_J23115 and PBAD

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. 4A3.PBAD-repL(AT2).J23115-gfp

    Plasmid

    #197873

    Purpose

    Arabinose induced expression of repL(AT2) gene, which triggers DNA replication in trans, weak level of constitutive GFP expression

    Depositor

    Baojun Wang

    Article

    Huan et al Front Microbiol. 2023 Feb 9;14:1095671.

    Insert

    part kilA/repL(AT2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSUMO His6 SUMO SnRK2.6 (11-362)

    Plasmid

    #177847

    Purpose

    Bacterial Expression of SnRK2.6

    Depositor

    Karsten Melcher

    Article

    Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

    Insert

    SNF1-related protein kinase 2.6 (OST1 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWUR703

    Plasmid

    #53082

    Purpose

    inducible TtAgoDM (D478A,D546A) expression

    Depositor

    John van der Oost

    Article

    Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

    Insert

    TtAgo
    

    Use

    Tags

    Strep-II tag

    Expression

    Bacterial

    Mutation

    D478A D546A

    Promoter

    T7

    Available since

    May 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28_6xHis-PARP1-CAT

    Plasmid

    #173943

    Purpose

    Bacterial expression of isolated PARP1 CAT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-CAT (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX5X.1 PINK1 WT

    Plasmid

    #13321

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 kinase domain (PINK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acids 112-496

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSC5

    Plasmid

    #188602

    Purpose

    Superior Conjugative Plasmid to Diverse Yeast

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge