30,260 results

1. pDEST-periHisMBP

   Plasmid

   #11086

   Depositor

   David Waugh

   Article

   Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

   Type

   Empty backbone

   Use

   Tags

   6xHis, MBP, and MBP signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   Tac (lactose/IPTG inducible)

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDD131

   Plasmid

   #119886

   Purpose

   Optimized aTc inducible pTet promoter for Rhodococcus opacus expressing mCherry on pAL5000/pMB1 backbone with kanamycin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

   Insert

   pTet_mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFPV-mCherry

   Plasmid

   #20956

   Depositor

   Olivia Steele-Mortimer

   Article

   Drecktrah et al Traffic. 2008 Dec . 9(12):2117-29.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTOX8

   Plasmid

   #127453

   Purpose

   Encodes MqsR toxin and tsPurple chromoprotein. CAM-R

   Depositor

   Matthew Waldor

   Article

   Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

   Inserts

   mqsR toxin
   tsPurple chromoprotein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized from protein sequence in FPbase

   Promoter

   apFAB46 and pRha

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-Cdk9

   Plasmid

   #28102

   Depositor

   Andrew Rice

   Article

   Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

   Insert

   Cdk9 (CDK9 Human)
   

   Use

   Tags

   3x HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAT217

   Plasmid

   #134885

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-MH6-RspCas13d_RspCasWYL1

   Plasmid

   #108304

   Purpose

   Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Inserts

   RspCas13d
   RspWYL1
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. His6_MBP_FXR1P

   Plasmid

   #198694

   Purpose

   Expression of human FXR1 protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FXR1P (FXR1 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)

   Plasmid

   #110560

   Purpose

   miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insert

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   stRBS-lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhaSR-PrhaBAD

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET30-2-PHGDH

    Plasmid

    #83907

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    Phosphoglycerate dehydrogenase (PHGDH Human)
    

    Use

    Tags

    6x His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pUCP20T-mko1

    Plasmid

    #78467

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    mKO1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pExp-GST

    Plasmid

    #112566

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-GST and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-SBP-TEV

    Plasmid

    #36943

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHistag-SBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCE27

    Plasmid

    #174405

    Purpose

    C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPLN-hFluc-WT-HA-GFP11-KDEL

    Plasmid

    #177722

    Purpose

    Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Use

    Tags

    GFP11, HA, KDEL, and Prolactin Signal Sequence

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTHSSe_50

    Plasmid

    #109248

    Purpose

    incW BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23105

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pS23·Pm-GFP

    Plasmid

    #122593

    Purpose

    Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    Pm promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG-Matr3 delRRM2

    Plasmid

    #32882

    Depositor

    Yossi Shiloh

    Article

    Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

    Insert

    MATR3 Matrin3 (MATR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    deleted amino acids 497-566

    Promoter

    CMV

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

19. PARP15-c021

    Plasmid

    #173102

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    G287-N470, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET22b-6h-GST-TEVp

    Plasmid

    #172887

    Purpose

    Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

    Depositor

    Urartu Seker

    Article

    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

    Insert

    TEV protease
    

    Use

    Tags

    GST solubility tag and HHHHHH

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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