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Bacterial Expression

29,765 results

Showing: 7251 - 7300 of 29765 results

  3. ATX3-77Q

    Plasmid
    #184249
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Synthetic, Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Codon optimization for protein expression in BL2…
    Promoter
    T7 promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    184249-plasmid-map-sequence-id-370367

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  12. KG#205

    Plasmid
    #110881
    Purpose
    Expresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1
    Inserts
    rab-3 promoter
    pde-4d (isoform d) D448N cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Asp 448 to Asn
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110881-plasmid-map-sequence-id-212568

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  14. pExp-NHis

    Plasmid
    #112558
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112558-plasmid-map-sequence-id-217235

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  18. CSNK1G1

    Plasmid
    #38934
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CSNK1G1 (CSNK1G1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pDB070.iodo.5

    Plasmid
    #99397
    Purpose
    Expression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanine
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    PN25 TetR; Ptet Iodo.5RS; PproK tyrT(Opt,CUA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PN25, Ptet, and PproK
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    99397-plasmid-map-sequence-id-198299

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  25. proinsulin-moxGFP2

    Plasmid
    #218957
    Purpose
    Expresses proinsulin fused to moxGFP2 in bacteria
    Depositor
    Benjamin Glick
    Insert
    proinsulin-moxGFP2
    Use
    Tags
    proinsulin
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218957-plasmid-map-sequence-id-446163

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  26. proinsulin-mGold

    Plasmid
    #218953
    Purpose
    Expresses proinsulin fused to mGold in bacteria
    Depositor
    Benjamin Glick
    Insert
    proinsulin-mGold
    Use
    Tags
    proinsulin
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218953-plasmid-map-sequence-id-446158

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  28. Dox-Akt

    Plasmid
    #207328
    Purpose
    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)
    Depositor
    Jared Toettcher
    Article
    Underhill et al Development. 2023 Aug 15;150(16):dev2016
    Insert
    rac protein kinase-alpha (AKT1 Human)
    Use
    Tags
    myristoylation tag - BFP
    Expression
    Bacterial
    Mutation
    range: 129 - 480
    Promoter
    Tet Response Element
    Available since
    Nov. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    207328-plasmid-map-sequence-id-413074

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  31. pET11b-MIF Y99F

    Plasmid
    #149439
    Purpose
    Expression of MIF Y99F in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y99F
    Promoter
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149439-plasmid-map-sequence-id-311578

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  38. pCol1a1-frt (FVB)

    Plasmid
    #63575
    Purpose
    Targeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.
    Depositor
    Ivo Huijbers
    Article
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Col1A-frt-hygro-pA
    Use
    Mouse Targeting; Frt/flpe
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    None
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63575-plasmid-map-sequence-id-105719

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  42. SIRT6

    Plasmid
    #41564
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SIRT6 (SIRT6 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    aa 3-318
    Promoter
    T7
    Available since
    April 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits

  44. pAC-crRNA-Km

    Plasmid
    #158712
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    AcGFP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TetO
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158712-plasmid-map-sequence-id-321925

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  45. pHL 246

    Plasmid
    #53014
    Purpose
    AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1
    Depositor
    Han Lim
    Article
    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    fimS (off orientation)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  47. pPICZa-STE2-1D4

    Plasmid
    #133436
    Purpose
    Mutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C59S/C252A
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133436-plasmid-map-sequence-id-267728

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  48. MECR

    Plasmid
    #38850
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MECR (Mecr Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  49. SNTB2

    Plasmid
    #38912
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SNTB2 (SNTB2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 7251 - 7300 of 29765 results