32,669 results
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Plasmid#41684DepositorInsertNpuDnaE intein
TagsGB1 and His-tagExpressionBacterialPromoterT7Available SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBBR1-kan-Ptet-plu-gamma-ET_rhi145
Plasmid#180427PurposeGenome engineering of AgrobacteriumDepositorInsertplu-gamma-ET_rhi145
ExpressionBacterialAvailable SinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pFCiR5
Plasmid#149479PurposeBroad-Host range expression vector, RSF1010 origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertCinR(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPCinAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCORE-UH
Plasmid#72239PurposeContains the CORE cassette with KlURA3 and hyg markersDepositorInsertsKlURA3
hyg
ExpressionBacterialAvailable SinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Pak1 83-149
Plasmid#12216DepositorInsertPak1 (Pak1 Rat)
TagsGSTExpressionBacterialMutationDominant negative Pak1: autoinhibitory domain, aa…Available SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-mNeonGreen-EC18153
Plasmid#137075PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAtetO_6xHis-v5.9
Plasmid#155259PurposePlasmid for the aTc-inducible expression of the v5.9DepositorInsertv5.9
Tags6xHisExpressionBacterialPromotertet PAAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-luxKate
Plasmid#229946PurposeBacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)DepositorInsertluxKate
TagsHis-tagExpressionBacterialPromoteraraBADAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rDAD2 cPGFP(G164)
Plasmid#165994PurposeExpression of coding sequence in Escherichia coliDepositorInsertrDAD2 cPGFP(G164)
ExpressionBacterialPromoterT7 promoterAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
23 pCOLI_A_N-SUMO_C_STREP
Plasmid#160259PurposeN-term His-SUMO 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vectorDepositorTypeEmpty backboneTags2xStrepII, 6xHis, and SUMOExpressionBacterialPromoterT7Available SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G6f
Plasmid#62811PurposepPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP6f-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp4
Plasmid#156474PurposeBacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tagDepositorInsertNsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-tdMCP-12xGCN4
Plasmid#205158Purposeexpress tdMCP-12xGCN4DepositorInserttdMCP-12xGCN4
ExpressionBacterial and MammalianPromoterCMVAvailable SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-WT-N’His
Plasmid#117701PurposeExpression of WT mouse SOD1 with N- terminal His tagDepositorAvailable SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H
Plasmid#118229PurposeEmpty E. coli - B. burgdorferi shuttle vector; hygromycin B resistantDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
371-GFP
Plasmid#97030PurposeHis6-GFP-tev-yORFDepositorTypeEmpty backboneTagsGFP fusion, His6 tag, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSC-PreE2
Plasmid#52264PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linkerDepositorAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET23a_mod_HuD_FL
Plasmid#65751Purposeplasmid for bacterial overexpression of HuDDepositorAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs1-SH2
Plasmid#46504DepositorInsertSocs1 (SOCS1 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-166PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-HsRCK_E
Plasmid#146210PurposeBacterial Expression of HsRckDepositorInsertHsRck (DDX6 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA2
Plasmid#52262PurposeBacterial expression of human 6His-tagged SUMO2 and SUMO-E1DepositorTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD-TDsmURFP-RBS-HO-1
Plasmid#80342PurposeExpresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial cells.DepositorInsertsTandem Dimer smURFP
HO-1
Tags6 Histidine TagExpressionBacterialPromoterpBADAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSG058
Plasmid#214261PurposePaFtsH1-6xHis cloned between XbaI/NheI in pET-28a(+)DepositorInsertCore genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
Tags6xHis-tagExpressionBacterialPromoterT7 promoterAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEN6-Ubc5 Sc
Plasmid#83234PurposepHis-par2-UbHs/Ubc5Sc/ Uba1TaDepositorAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CDC45_dTAG
Plasmid#232733PurposeGenerates CDC45 dTAG C ternimal knock-in cell lines as donorDepositorAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX459_MCM2_sgRNA
Plasmid#232730PurposeGenerates MCM2 dTAG C ternimal knock-in cell linesDepositorAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX459_GINS4_gRNA
Plasmid#232734PurposeGenerates GINS4 dTAG C ternimal knock-in cell linesDepositorAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX459_CDC45_sgRNA
Plasmid#232732PurposeGenerates CDC45 dTAG C ternimal knock-in cell linesDepositorAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19_GINS4_dTAG
Plasmid#232735PurposeGenerates GINS4 dTAG C ternimal knock-in cell lines as donorDepositorAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19_MCM2_dTAG
Plasmid#232731PurposeGenerates MCM2 dTAG C ternimal knock-in cell lines as donorDepositorAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 IQGAP1 WW domain
Plasmid#104279PurposeBacterial expression of WW domain from IQGAP1DepositorInsertIQGAP1 WW domain (IQGAP1 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL0762 (pQCascade_crRNA-nt)
Plasmid#130636PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT21
Plasmid#89662Purposeexpression of doubleTAL staple protein dT21 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT21
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3CA
Plasmid#47069PurposeFX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tagDepositorTypeEmpty backboneTags3C protease site (PreScission site), AVI-tag, and…ExpressionBacterialPromoteraraBADAvailable SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pDR111_mannose_Bgal no NLS
Plasmid#188399PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptideExpressionBacterialPromoterD-mannoseAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD_mCherry
Plasmid#187387Purposearabinose-inducible expression of mCherryDepositorInsertmCherry
ExpressionBacterialAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATG-1929
Plasmid#108712PurposeCBR2opt in pF4Ag expression vector with T7 and CMV promoterDepositorInsertCBR2opt
ExpressionBacterial and MammalianMutationPromega used directed evolution to create CBR (Cl…PromoterT7, CMVAvailable SinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHdCas9
Plasmid#137080PurposeExpression of Himar-dCas9 in bacteriaDepositorInsertHimar-dCas9
ExpressionBacterialPromoterpLtetO-1Available SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-EGFP-NLS-3XFLAG-V5
Plasmid#196090PurposeExpresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertGFP
TagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianPromoterCAGGsAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT9 WT
Plasmid#67607Purposebacterial expression of wild type human PRMT9DepositorAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Plac-SO
Plasmid#218358PurposeEmpty vector with lacIq-Plac expression systemDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPReporter-mCherry
Plasmid#65008PurposeE. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterUser-definedAvailable SinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJH674_pTech_PlldR-HHRz-supP
Plasmid#163728PurposeLactate-inducible expression of supP, preceded by a hammerhead ribozymeDepositorInserthhrz-supP
ExpressionBacterialAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT32
Plasmid#89667Purposeexpression of doubleTAL staple protein dT32 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT32
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET24a-AlkB deltaN11
Plasmid#73622PurposeExpresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tagDepositorInsertAlkB (alkB E. coli)
TagsHisExpressionBacterialMutationdeletion of first 11 AAPromoterT7Available SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDFNKI-strepII-3C-LIC-strep
Plasmid#108708PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsStrepII-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAbaMCi-GFP-BsaI_pJMP2748
Plasmid#208888PurposeAcinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).DepositorInsertdCas9, sgRNA expression cassette, sfGFP
UsePir dependent origin of replicationExpressionBacterialAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT16
Plasmid#89659Purposeexpression of doubleTAL staple protein dT16 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT16
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only