32,669 results
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Plasmid#110753PurposeBacterial Expression of RNF144B RBR domainDepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only
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pExp-TF
Plasmid#112575PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis-TriggerFactor and Strep tagExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPEPY
Plasmid#122633PurposeBglFusion compatible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXRM15
Plasmid#63150PurposeMet15 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxExpressionBacterial and YeastAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-WT-HA-GFP11-KDEL
Plasmid#177722PurposeFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFluc
TagsGFP11, HA, KDEL, and Prolactin Signal SequenceExpressionBacterial and MammalianAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPEPX-Plac
Plasmid#122632PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU
Plasmid#64180Purposeharbours marker gene URA3DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
p007ampGFP
Plasmid#58535PurposeGFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.DepositorInsertGreen fluorescent protein
ExpressionBacterialPromoterT5 between two lac operatorsAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCPP3416
Plasmid#128720PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopT1-1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5534
Plasmid#128723PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopX1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+bromo5
Plasmid#157803PurposeExpression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1DepositorInsertbromo5
TagsHis6 and MBPExpressionBacterialPromoterpTACAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
wt 6XHis-SNX9 pET15b
Plasmid#34690DepositorInsertSorting nexin 9
Tags6xHisExpressionBacterialAvailable SinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5e-Rubicon RH
Plasmid#216706PurposeBacterial protein expression of Rubicon RH domainDepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn-pOUT
Plasmid#213905PurposeMariner transposon with outward-facing pJ23104 promoterDepositorInsertmariner transposon with kan resistance and outward-facine pJ23104 promoter
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCoofy3
Plasmid#43983DepositorTypeEmpty backboneTagsHis6-GSTExpressionBacterialPromoterT7Available SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP7 (catalytic domain)
Plasmid#16360DepositorInserthuman SENP7 catalytic domain (SENP7 Human)
TagsHisExpressionBacterialMutationAmino Acid 640 to 984 catalytic domain of human S…Available SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAOJ15
Plasmid#91567PurposeBroad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoterDepositorTypeEmpty backboneExpressionBacterialPromoterTetracycline promoterAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_A321V
Plasmid#98671PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with A321VDepositorAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET His6 ProteinG TEV LIC cloning vector (2P-T)
Plasmid#29713DepositorTypeEmpty backboneTagsHis6-ProteinG-TEVExpressionBacterialAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAM105
Plasmid#86479PurposeExpresses p9-FLAG-FMODepositorInsertp9-FLAG-FMO
UseSynthetic BiologyTagsFLAG and FMOExpressionBacterialPromoterpTetAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-ZFD_L
Plasmid#198276PurposeFor loading L half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Left-G1397-N_DddA); also contains PVC regulatory genesDepositorInsertPdp1_NTD-ZFD_L
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GAS7-1/1
Plasmid#91301PurposeProtein expression and purification of human SH3 domain construct GAS7-1/1DepositorInsertGAS7-1/1 (GAS7 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)
Plasmid#48283DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
G3BP1A
Plasmid#42480PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertG3BP1A
TagsHis6-TEVExpressionBacterialMutationSee CommentsAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
k3(long) luciferase
Plasmid#11110DepositorAvailable SinceApril 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMRE147
Plasmid#118499Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmClover3
ExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK351
Plasmid#129696PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120034435
ExpressionBacterialAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBXCA3H
Plasmid#47072PurposeFX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tagDepositorTypeEmpty backboneTags3C protease site (PreScission site), Avi-tag, and…ExpressionBacterialPromoteraraBADAvailable SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
human PNP non-tagged
Plasmid#64199PurposeExpression of native human PNP (non-tagged)DepositorAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
AMPKA2
Plasmid#36194PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAMPKA2 (PRKAA2 Human)
TagsHis tag with thrombin cleavageExpressionBacterialMutationcontains KINASE DOMAIN onlyAvailable SinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRST11B-AS3_4
Plasmid#102789PurposeExpresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNADepositorInsertsExpressionBacterialAvailable SinceJuly 3, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRECMYC-Ebu1
Plasmid#240713PurposepLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155DepositorInsertEbu1 RT, Eco1 ncRNA, MspRecT
ExpressionBacterialMutationWTAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJAK184.tetR.PT5-3.dCas9
Plasmid#232482Purposeoptimized ahTet inducible dCas9 for insertion into C. difficile genomeDepositorInsertCatalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
UseCRISPRExpressionBacterialPromoterTetR-PT5-3Available SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJC.15A.sgRNA.TA
Plasmid#232483PurposesgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.DepositorInsertsToxin-antitoxin System from CD630
medium-copy-number p15A origin of replication
mCherry
UseCRISPRExpressionBacterialPromoterP3 constitutive promoter and native promoterAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCW-LIC-N8C_Fab2b
Plasmid#203692PurposeExpresses N8C_Fab2bDepositorInsertN8C_Fab2b
ExpressionBacterialAvailable SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pE5770
Plasmid#170777Purposeexpression of hybrid proteins in E. coliDepositorAvailable SinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMesh1
Plasmid#175594PurposeLac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
ExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEE075
Plasmid#176832PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert714
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pASR103
Plasmid#122637PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-Tn5-F
Plasmid#160081PurposeBacterial expression of C-terminally FLAG-tagged Tn5 transposaseDepositorInsertTn5 transposase
TagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWVR5
Plasmid#167509PurposeBroad-Host range expression vector, pSa origin, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertVanRAM-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPVanCCAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEA01
Plasmid#170766PurposeUtilisation of pSAM2 excision tool in Amycolatopsis sppDepositorInsertsxis, int
oriT
pac
ExpressionBacterialPromotertrcpAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
NpuDnaEc-cGCaMP2
Plasmid#34851DepositorInsertNpuDnaEc-cGCaMP2
ExpressionBacterial and MammalianPromoterCMV, T7 lacAvailable SinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Zeo-eyfp
Plasmid#65039PurposeZeoR mini-Tn7 vector, mobilizable, for YFP tagging bacteriaDepositorInserteyfp
ExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(cAMP-1.6)/pRSETB
Plasmid#53591PurposeNano-lantern-based luminescent cAMP indicatorDepositorInsertNano-lantern-based luminescent cAMP indicator
Tags6xHisExpressionBacterialAvailable SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-ShoHELIX-KanR (BO4)
Plasmid#181795PurposepDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
ExpressionBacterialAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CohIII-CBM-HIS-ybbR
Plasmid#60866PurposeCharacteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tagDepositorInsertsCohIII
CBM
Tags6xHIS, HRV 3C, and ybbRExpressionBacterialMutationCysteine 63 to Serine in CBM modulePromoterT7Available SinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEZYHb
Plasmid#18669PurposepKW32 converted Gateway Destination vector for expressing hemoglobin tagged fusion proteins in bacteriaDepositorTypeEmpty backboneTagshemoglobinExpressionBacterialAvailable SinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
PG1424PGA
Plasmid#74652PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPG1424PGA
TagsHis-6-TEVExpressionBacterialPromoterT7Available SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AT110.i1
Plasmid#135481PurposeE. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptorDepositorInsertNanobody AT110.i1
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only