30,744 results

1. pDSG372

   Plasmid

   #115602

   Purpose

   surface-displayed Nb1; medium copy

   Depositor

   Ingmar Riedel-Kruse

   Article

   Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

   Insert

   regulated adhesin construct
   

   Expression

   Bacterial

   Available Since

   Oct. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. IL-4 promoter luciferase

   Plasmid

   #12195

   Depositor

   Anjana Rao

   Article

   Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

   Insert

   IL-4 promoter (Il4 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Bacterial

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTrcHIS-7D12Gln13TAG

   Plasmid

   #125264

   Purpose

   Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF

   Depositor

   Angus Johnston

   Article

   Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

   Insert

   Gln13TAG 7D12
   

   Tags

   His-Tag

   Expression

   Bacterial

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRha-ABE7.10-SpCas9-NG

   Plasmid

   #170663

   Purpose

   Expresses the base editor ABE7.10-SpCas9-NG in bacterial cells

   Depositor

   David Liu

   Article

   Krishnamurthy et al Nucleic Acids Res. 2021 Oct 11;49(18):10

   Insert

   ABE7.10-SpCas9-NG
   

   Tags

   8xHIS and BP-NLS

   Expression

   Bacterial

   Promoter

   pRhaBAD

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMM656

   Plasmid

   #68887

   Purpose

   Rhamnose-inducible NanoLuc expression, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Insert

   NanoLuc
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET32a-HD25Q

   Plasmid

   #11508

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 25 glutamines (HTT Human)
   

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 25 glutamines, vector m…

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcI-ts,ind+-(mtSSB delta20)

   Plasmid

   #186280

   Purpose

   Expresses Human Mitochondrial SSB in E. coli

   Depositor

   Kenneth Johnson

   Article

   Qian et al J Biol Chem. 2017 Aug 4;292(31):13068-13

   Insert

   Mitochondrial Single Stranded DNA Binding Protein (SSBP1 Human)
   

   Expression

   Bacterial

   Mutation

   First 20 amino acids corresponding to probable mi…

   Promoter

   lambda

   Available Since

   July 6, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. GST-mDia1(FH1FH2DAD)-His

   Plasmid

   #85822

   Purpose

   contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.

   Depositor

   Guillaume Romet-Lemonne, Antoine Jégou

   Article

   Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc

   Insert

   Protein diaphanous homolog 1 (Diaph1 Mouse)
   

   Tags

   8*His and GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRAM24

   Plasmid

   #234313

   Purpose

   pBBR1 origin cat-RNA U1376

   Depositor

   James Chappell

   Article

   Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

   Insert

   U1367 Targeting RAM, Barcode 1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   P. CymRC

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLsCas13a-gRNA-NT

    Plasmid

    #164857

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-NT
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. His-GST-TEV-BRD4(42-459) BD1-BD2

    Plasmid

    #233887

    Purpose

    pGex for protein overexpression of human BRD4

    Depositor

    Alessio Ciulli

    Article

    Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

    Insert

    BRD4 (BRD4 Human)
    

    Tags

    His-GST-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-Cdk9

    Plasmid

    #28100

    Depositor

    Andrew Rice

    Article

    Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

    Insert

    Cdk9 (CDK9 Human)
    

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-MH6-EsCas13d

    Plasmid

    #108303

    Purpose

    Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Insert

    EsCas13d
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pN565

    Plasmid

    #49990

    Purpose

    expresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible control

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    T7 RNAP*
    

    Use

    Synthetic Biology

    Tags

    umuD degradation tag

    Expression

    Bacterial

    Mutation

    R632S

    Promoter

    pTac-symmetric

    Available Since

    Jan. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACYC-FLAG-dN6-His

    Plasmid

    #22143

    Depositor

    Robert Sauer

    Article

    Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

    Insert

    Flag-clpXdeltaNLinkedHexamer-His6
    

    Tags

    Flag, His6, and L20 Linkers

    Expression

    Bacterial

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET20b-delta133HtrA2

    Plasmid

    #14126

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2003 Dec 5. 278(49):49417-2

    Insert

    HtrA2 (HTRA2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. p404ADH1

    Plasmid

    #15970

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    ADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    ADH1 promoter is between SacI and XbaI restrictio…

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. Pediculus humanus corporis PINK1 (115 - 575)

    Plasmid

    #110750

    Purpose

    Bacterial expression of PhPINK1 (biochemistry)

    Depositor

    David Komander

    Article

    Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

    Insert

    Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
    

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. ICMAB - pET22b T7 EncFtn Mms6

    Plasmid

    #225538

    Purpose

    Construction of intracellular magnetite accumulating bacterial systems.

    Depositor

    Urartu Seker

    Article

    Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

    Inserts

    Iron binding protein
    Ferroxidase
    Material binding peptite
    Iron transporter protein
    

    Tags

    no

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2)

    Plasmid

    #169187

    Purpose

    To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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