Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,656 results

Showing: 801 - 850 of 32656 results

  7. pMSP3545

    Plasmid
    #46888
    Purpose
    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression
    Depositor
    Gary Dunny
    Article
    Bryan et al Plasmid. 2000 Sep;44(2):183-90.
    Type
    Empty backbone
    Use
    Nisin-inducible expression; gram-positive bacteri…
    Expression
    Bacterial
    Promoter
    PnisA
    Available Since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    46888-plasmid-map-sequence-id-347882

    Enlarge

  11. His6-pAG-RTase

    Plasmid
    #214932
    Purpose
    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems
    Depositor
    Chuan He
    Article
    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s
    Insert
    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…
    Promoter
    T7 promoter
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    214932-plasmid-map-sequence-id-426479

    Enlarge

  12. pNAS1b split mCherry 14-3-3β ctrl

    Plasmid
    #112031
    Purpose
    Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
    Tags
    Split mCherry
    Expression
    Bacterial
    Available Since
    July 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112031-plasmid-map-sequence-id-216275

    Enlarge

  13. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid
    #119946
    Purpose
    Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18
    Insert
    Renilla luciferase
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Promoter
    TetCMV
    Available Since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    119946-plasmid-map-sequence-id-237566

    Enlarge

  16. cBEST4

    Plasmid
    #234660
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3 and tcp830
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234660-plasmid-map-sequence-id-472864

    Enlarge

  25. pOpen_taq

    Plasmid
    #153315
    Purpose
    Bacterial expression of Taq polymerase
    Depositor
    Drew Endy
    Insert
    Taq polymerase
    Expression
    Bacterial
    Promoter
    Tac
    Available Since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153315-plasmid-map-sequence-id-298774

    Enlarge

  26. Zika NS5 RdRp

    Plasmid
    #204788
    Purpose
    Bacterial expression of Zika NS5 RdRp
    Depositor
    ASAP Discovery, Frank von Delft
    Insert
    Zika NS5 RdRp domain
    Expression
    Bacterial
    Available Since
    April 25, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    204788-plasmid-map-sequence-id-417058

    Enlarge

  27. mAIRN HO

    Plasmid
    #207411
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Expression
    Bacterial and Mammalian
    Available Since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207411-plasmid-map-sequence-id-422696

    Enlarge

  28. mAIRN CD

    Plasmid
    #207412
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Expression
    Bacterial and Mammalian
    Available Since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207412-plasmid-map-sequence-id-422689

    Enlarge

  30. pLyGo-Ec-7

    Plasmid
    #163135
    Purpose
    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Nanobody, C-IgAP autotransporter
    Expression
    Bacterial
    Promoter
    P(rhaB)
    Available Since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    163135-plasmid-map-sequence-id-332144

    Enlarge

  32. pDule2-3-nitroTyrosine (A7)

    Plasmid
    #174079
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174079-plasmid-map-sequence-id-341130

    Enlarge

  35. p006-GFP-strong

    Plasmid
    #108313
    Purpose
    GFP from jellyfish Aquorea sp. under strong synthetic constitutive promoter pFAB4026; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Green fluorescent protein
    Expression
    Bacterial
    Promoter
    pFAB4026
    Available Since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    108313-plasmid-map-sequence-id-459279

    Enlarge

  37. ECFP HO

    Plasmid
    #207413
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207413-plasmid-map-sequence-id-421043

    Enlarge

  38. ECFP CD

    Plasmid
    #207414
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207414-plasmid-map-sequence-id-421036

    Enlarge

  39. DYRK1A

    Plasmid
    #38913
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DYRK1A (DYRK1A Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  43. pTU2-AmyE-Pveg-eGFP

    Plasmid
    #112792
    Purpose
    Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    Pveg-eGFP
    Expression
    Bacterial
    Available Since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112792-plasmid-map-sequence-id-218394

    Enlarge

Showing: 801 - 850 of 32656 results