29,690 results

1. pQE60-RRF-His

   Plasmid

   #124132

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Ribosome recycling factor (frr Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEM-Cas9HF1-recA56

   Plasmid

   #89962

   Purpose

   Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Inserts

   Cas9HF1
   recA56
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet and proD

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Pramr-GFP

   Plasmid

   #194148

   Purpose

   Contains a RamR-regulated promoter expressing GFP

   Depositor

   Andrew Ellington

   Article

   d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pramr

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PT7lacO-RF1-His6

   Plasmid

   #224372

   Purpose

   Express RF1 protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   RF1 (prfA E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pY2ShHELIX_sgRNA_entry (CJT30)

   Plasmid

   #181781

   Purpose

   Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. miniTn7(Gm)PA1/04/03-eyfp-a

   Plasmid

   #111620

   Purpose

   eYFP Fluorescent tagging of bacteria at their Tn7-site

   Depositor

   Tim Tolker-Nielsen

   Article

   Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

   Insert

   PA1/04/03-eyfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RepairTemplate_NIPBL_mEGFP_mAID

   Plasmid

   #217664

   Purpose

   Repair template to endogenously tag NIPBL with mEGFP-mAID Tag

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   NIPBL_mEGFP_mAID (NIPBL Human)
   

   Use

   Tags

   mAID and meGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

   Plasmid

   #217662

   Purpose

   expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pH6HTC_TKT-Halo

   Plasmid

   #175331

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   TKT (TKT Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTrc99a-NoSS-TolC

    Plasmid

    #155179

    Purpose

    Expresses signal sequence-less TolC as inclusion bodies

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    TolC (tolC )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted residues 1-20, residue 21 mutated to Met …

    Promoter

    trc

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-3c-rhVTN-N

    Plasmid

    #30225

    Depositor

    James Thomson

    Article

    Chen et al Nat Methods. 2011 Apr 10. ():.

    Insert

    rhVitronectin with N terminus cleavage (VTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids: 20*-62

    Promoter

    Available since

    May 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Cys-30nmSAH-SPYcatcher

    Plasmid

    #214839

    Purpose

    This plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Cys-30nmSAH-SPYcatcher
    

    Use

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCR-XL-rAkap9

    Plasmid

    #196867

    Purpose

    Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Akap9 (Akap9 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac promoter and T7 promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRBC-sfGFP-150TAG

    Plasmid

    #174076

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Use

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    T7

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET His6 TEV LIC cloning vector (1B)

    Plasmid

    #29653

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJOE6261.2

    Plasmid

    #135079

    Purpose

    P. putida KT2440 rescue construct

    Depositor

    Josef Altenbuchner

    Article

    Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

    Insert

    upp (upp Pseudomonas putida)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRAV16

    Plasmid

    #123389

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV16 (TRAV16 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TRAV13-1

    Plasmid

    #123386

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV13-1 (TRAV13-1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNVLTv2-pvdIJD-Prha-base

    Plasmid

    #169244

    Purpose

    Suicide vector for integrating expression cassette in P. putida (L-rhamnose induction)

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    rhaRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaS

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSET-iGluSnFR4f

    Plasmid

    #234444

    Purpose

    Bacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast)

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f
    

    Use

    Tags

    6xHis-Xpress epi

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHH0103_SPF45

    Plasmid

    #110044

    Purpose

    Protein expression and purification of SPF45

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    SPF45 (RBM17 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TRBV18

    Plasmid

    #123452

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV18 (TRBV18 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TRBV15

    Plasmid

    #123450

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV15 (TRBV15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TRBV10-3

    Plasmid

    #123441

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV10-3 (TRBV10-3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TRBV7-9

    Plasmid

    #123437

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV7-9 (TRBV7-9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TRBV7-2

    Plasmid

    #123431

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV7-2 (TRBV7-2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TRBV6-1

    Plasmid

    #123421

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV6-1 (TRBV6-1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TRBV4-2

    Plasmid

    #123412

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV4-2 (TRBV4-2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TRBV4-1

    Plasmid

    #123411

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV4-1 (TRBV4-1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TRAV38-2

    Plasmid

    #123405

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV38-2 (TRAV38-2DV8 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TRAV26-1

    Plasmid

    #123398

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV26-1 (TRAV26-1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TRAV22

    Plasmid

    #123394

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV22 (TRAV22 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TRAV20

    Plasmid

    #123392

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV20 (TRAV20 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. TRBV3-1

    Plasmid

    #123410

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV3-1 (TRBV3-1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. TRAV8-2

    Plasmid

    #123376

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV8-2 (TRAV8-2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TRAV4

    Plasmid

    #123371

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV4 (TRAV4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TRAV1-2

    Plasmid

    #123368

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV1-2 (TRAV1-2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJK226

    Plasmid

    #71125

    Purpose

    Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    citrate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pETduet+sfGFP-TetR

    Plasmid

    #157794

    Purpose

    Expression of sfGFP-TetR fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    sfGFP-TetR
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGEX-αTAT1[2-236]

    Plasmid

    #27101

    Depositor

    Maxence Nachury

    Article

    Shida et al Proc Natl Acad Sci U S A. 2010 Nov 10. (

    Insert

    αTubulin K40 acetyltransferase (ATAT1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. 6xHis-SNAP-PI(4,5)P2 Probe

    Plasmid

    #211513

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    PLCdelta1-PH
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET28a-sfGFP

    Plasmid

    #85492

    Purpose

    pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. 6xHis-SNAP-PI(4)P Probe

    Plasmid

    #211509

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    SidC (P4C)
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET29-bdSUMO-Anti-ALFA nanobody

    Plasmid

    #212311

    Purpose

    Expression of anti-ALFA nanobody

    Depositor

    Arthur Laganowsky

    Article

    Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pEvoltac-EcW-TGA-h14

    Plasmid

    #218764

    Purpose

    encodes EcWRS-h14 and EcW-tRNA-TGA

    Depositor

    Abhishek Chatterjee

    Article

    Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

    Insert

    EcWRS-h14
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S8A, V144G, V146C

    Promoter

    tacI

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. NPY-GFP

    Plasmid

    #74629

    Purpose

    human neuropeptide Y fused to GFP

    Depositor

    Justin Taraska

    Insert

    Neuropeptide Y (NPY Human)
    

    Use

    Tags

    mEGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pKnockinDonor-human-p16INK4A-P2A-mCherry

    Plasmid

    #137026

    Purpose

    donor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A

    Depositor

    Chunliang Li

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2019 Dec 9. pi

    Insert

    p16INK4A-knockin-homology arm-P2A-mCherry
    

    Use

    Tags

    P2A-mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    no promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. Cas12aUltra_5XNLS

    Plasmid

    #218775

    Purpose

    His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS

    Depositor

    Ross Wilson

    Article

    Sahu et al bioRxiv 2024.07.14.603391

    Insert

    5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
    

    Use

    CRISPR

    Tags

    6XHis (C terminal), HA, Nucleoplasmin, SV40, and …

    Expression

    Bacterial

    Mutation

    Nucleotides mutations were made to encode for arg…

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pJ404-DogCatcher-sfGFP

    Plasmid

    #171930

    Purpose

    Express DogCatcher-superfolder GFP protein in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    DogCatcher-sfGFP
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pTriEx4-H6-FGAm (FlincG3)

    Plasmid

    #49202

    Purpose

    Fluorescent reporter for cGMP

    Depositor

    John Garthwaite

    Article

    Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

    Insert

    FlincG3
    

    Use

    Tags

    His tag + other tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Met to Lys in cpEGFP region similar to CGaMP3

    Promoter

    CMV

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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