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pB025
Plasmid#183092PurposeExpresses FnCas12a in Bacteroides and used for genome editingDepositorInsertFnCas12a, gRNA, Promoter, HAB, TetR,
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP1TDPGH023Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC NLS HA stdMCP stdHalo
Plasmid#104999PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labelingDepositorInsertstdMCP-stdHalo
UseLentiviralTagsHA tag, NLS, Two synonymous copies of the Halo-ta…ExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
hPU.1ΔN165-pET28b
Plasmid#199796PurposeExpressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coliDepositorInsertspi1 (SPI1 Human)
UseTagsNoneExpressionBacterialMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRK892
Plasmid#71803PurposeExpression of mutated L. gasseri gusA from strong Ppgm promoterDepositorInsertgusA3
UseTagsExpressionBacterialMutationD573APromoterPpgmAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
p6XHis-T7(P266L)
Plasmid#174866PurposeExpresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.DepositorInsertT7 RNA polymerase
UseTags6XHis-tagExpressionBacterialMutationP266LPromoterlac promoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4 R105A
Plasmid#196072PurposeExpress mutant (R105A) SG4 in E.coliDepositorInsertSG4 R105A
UseTags3XFLAG and 6XHisExpressionBacterialMutationR105A mutation in SG4PromoterT7Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30a-HS-Tn5
Plasmid#127916PurposeProkaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.DepositorInsertHyperstable Tn5
UseTagsE coli elongation factor Ts and Mxe intein - Chit…ExpressionBacterialMutationE54K, L372PPromoterT7Available sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7II-2N4Rtau
Plasmid#177653PurposeRecombinant protein synthesis of human TauDepositorInsertTau (2N4R) (MAPT Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-Int
Plasmid#50000PurposeExpresses the integrase on a suicide plasmidDepositorInsertIntegrase from pMV361
UseTagsExpressionBacterialMutationPromoterownAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD_GFP11_T7LysH17A
Plasmid#59591PurposeExpression vector for solubility screening of proteins or protein fragments using a split-GFP assay.DepositorTypeEmpty backboneUseTags6xHis-tag and GFP11ExpressionBacterialMutationPromoterpARAAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDB1 (CK2alpha)
Plasmid#27083DepositorInsertCK2alpha (CSNK2A1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAB46 (CK2beta)
Plasmid#27085DepositorInsertCK2beta (CSNK2B Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA
Plasmid#119945PurposeExpresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorInsertRenilla luciferase
UseTags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-ABK
Plasmid#126035PurposeEfficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.DepositorInsertstRNA synthetase 1
tRNA synthetase 2
tRNA for TAG codon
UseTagsExpressionBacterialMutationL274M, C313A, Y349FPromoteraraBAD promoter, glnS promoter, and proK promoterAvailable sinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29b-H6_Streptavidin_sfGFP
Plasmid#124296PurposeHis6-tagged Streptavidin GFP fusion proteinDepositorInsertHis6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
UseTagsHis6 and T7 tagsExpressionBacterialMutationPromoterAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYTK095
Plasmid#65202PurposeEncodes AmpR-ColE1 as a Type 678 part to be used in the Dueber YTK systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-His
Plasmid#119755PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.DepositorTypeEmpty backboneUseTags10xHis and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4
Plasmid#196071PurposeExpress wild type SG4 in E.coliDepositorInsertSG4
UseTags3XFLAG and 6XHisExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEMG-GRIS
Plasmid#225134PurposeFor genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
