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psfaMs-Tn5

Plasmid

#183637

Purpose

Expresses anti-mouse nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Publication

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti mouse secondary nanobody fused to Tn5

Tags

anti-mouse nanobody

Expression

Bacterial

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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SepRS(2)/pSertRNA(B4)/EF-Sep

Plasmid

#173897

Purpose

The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

Depositor

Jason W Chin

Publication

Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

Inserts

SepRS(2)
pSertRNA
EF-Sep

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)

Plasmid

#29722

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), TEV cleavage site, and mC…

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pTriEx-mCherry-PA-Rac1-C450A

Plasmid

#22028

Purpose

Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

Depositor

Klaus Hahn

Publication

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pH2B_mCherry_IRES_neo3

Plasmid

#21044

Depositor

Daniel Gerlich

Publication

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pH2B

Tags

mCherry

Expression

Bacterial and Mammalian

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pCRISPR-PE-bacteria

Plasmid

#172715

Purpose

Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

Depositor

Tilmann Weber

Publication

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

Cas9n-linker-M-MLV2

Expression

Bacterial

Mutation

H840A of Cas9

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-32a(+)-TGFB3

Plasmid

#120288

Purpose

Produce human recombinant TGFB3 in DE3 cells

Depositor

Paul Burridge

Publication

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Transforming Growth Factor-β3 (TGFB3 Synthetic, Human)

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pKM461

Plasmid

#108320

Purpose

For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Inserts

Che9C RecT
Bxb1 Integrase
Tet Repressor
SacRB

Expression

Bacterial

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCMV-Tag4A-MITF-M (wt)

Plasmid

#31151

Depositor

Yardena Samuels

Publication

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

WT

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMV306hsp+LuxG13

Plasmid

#26161

Depositor

Brian Robertson, Siouxsie Wiles

Publication

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Bacterial luciferase operon + G13 promoter

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

40 Articles

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pMBIS

Plasmid

#17817

Depositor

Jay Keasling

Publication

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase

Use

Synthetic Biology

Expression

Bacterial

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET_RTX

Plasmid

#102787

Purpose

Expresses the Reverse Transcriptase gene

Depositor

Andrew Ellington

Publication

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase

Expression

Bacterial

Promoter

T7 promoter + lac operator

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSAM_Ec

Plasmid

#102939

Purpose

Transposon mutagenesis and Tn-seq

Depositor

Matthew Mulvey

Publication

Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

Insert

mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase

Expression

Bacterial

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMA7CR_2.0

Plasmid

#73950

Purpose

pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

Depositor

Alex Nielsen

Publication

Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

Inserts

cas9
lambda beta
dam
recX

Expression

Bacterial

Promoter

pAra and pTet

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET22b RNase A

Plasmid

#58903

Purpose

Bacterial expression of bovine pancreatic ribonuclease A

Depositor

Ronald Raines

Publication

delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

Insert

RNase A (RNASE1 Bovine)

Tags

pelB sequence

Expression

Bacterial

Mutation

See Depositor Comments below

Promoter

T7

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRSETA-PAGFP

Plasmid

#11911

Depositor

Jennifer Lippincott-Schwartz

Publication

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Insert

pRSETA-PAGFP

Expression

Bacterial

Mutation

The PAGFP has four mutations (L64F/T65S/V163A/T20…

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET17_K560_GFP_His

Plasmid

#15219

Depositor

Ron Vale

Publication

Woehlke et al Cell. 1997 Jul 25. 90(2):207-16.

Insert

Human neuronal kinesin heavy chain (KIF5B Human)

Tags

GFP and His

Expression

Bacterial

Available since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pRE112

Plasmid

#43828

Depositor

Dieter Schifferli

Publication

Edwards et al Gene. 1998 Jan 30;207(2):149-57.

Type

Empty backbone

Use

Bacterial allelic exchange vector with sacb1

Expression

Bacterial

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pJR98

Plasmid

#187239

Purpose

CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites

Expression

Bacterial

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pQE 80L Kan T7WT

Plasmid

#102790

Purpose

Expresses T7 RNA polymerase

Depositor

Andrew Ellington

Publication

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

T7 RNA polymerase

Expression

Bacterial

Available since

July 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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