29,813 results

1. pET29b_1_anti-GFP-nanobody

   Plasmid

   #210394

   Purpose

   Express anti-GFP nanobody in E. coli

   Depositor

   Magnus Rueping

   Article

   Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

   Insert

   1_anti-GFP-nanobody
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pET20b- hTPI

   Plasmid

   #50723

   Purpose

   overexpression of human TPI1 in E.coli

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Use

   Tags

   6xHIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. TDP-43_CTD

   Plasmid

   #98669

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET22b-6h-GST-TEVp

   Plasmid

   #172887

   Purpose

   Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Insert

   TEV protease
   

   Use

   Tags

   GST solubility tag and HHHHHH

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCE27

   Plasmid

   #174405

   Purpose

   C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.

   Depositor

   Clarissa Nobile

   Article

   Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

   Insert

   NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCE35

   Plasmid

   #174409

   Purpose

   C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.

   Depositor

   Clarissa Nobile

   Article

   Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

   Insert

   C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-C3-hYAP1

   Plasmid

   #17843

   Depositor

   Marius Sudol

   Article

   Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. Rb-379-928-pGex2TK

   Plasmid

   #21749

   Depositor

   William Kaelin

   Article

   Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deletion mutant ORF contains aa 379-928 of wt Rb …

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST-mCh-FYVE

   Plasmid

   #192289

   Purpose

   Expresses GST- and mCherry-tagged HRS FYVE in e Coli

   Depositor

   James Hurley

   Article

   Jensen et al Sci Adv. 2022 Dec 14;8(50):eadd1436. doi

   Insert

   HRS FYVE domain
   

   Use

   Tags

   GST-TEV-mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWM_12x601_25bpLinker

    Plasmid

    #157788

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_25bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS-slpGFP

    Plasmid

    #27171

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS-QUE7mu

    Plasmid

    #129311

    Purpose

    Low concentration ATP measurement

    Depositor

    Hiroyuki Noji

    Article

    Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

    Insert

    QUEEN-7mu
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHH0103_GNAQ_G-alpha

    Plasmid

    #109809

    Purpose

    Protein expression and purification of GNAQ_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNAQ_G-alpha (GNAQ Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pgex-cIAP1

    Plasmid

    #8328

    Depositor

    Jon Ashwell

    Article

    Li et al Nature 2002 Mar 21;416(6878):345-7.

    Insert

    cIAP1 (BIRC2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pET51b(+)_EGFP_NbALFA

    Plasmid

    #136626

    Purpose

    Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLxR5

    Plasmid

    #149465

    Purpose

    Broad-Host range expression vector, RK2 and pUC origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    LuxR-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLuxB

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a FKBP12-V5

    Plasmid

    #194117

    Purpose

    Expresses FKBP12 protein with V5 tag

    Depositor

    Jun Liu

    Insert

    FKBP1A (FKBP1A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LVXN-Neo-NSD2

    Plasmid

    #86010

    Purpose

    express wild-type NSD2 in mammalian cells

    Depositor

    Darrin Stuart

    Article

    Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

    Insert

    NSD2 (NSD2 Human)
    

    Use

    Lentiviral

    Tags

    Flag tag D Y K D D D D K

    Expression

    Bacterial

    Mutation

    K1002R (please see depositor comments below)

    Promoter

    CMV

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_GNAI2_G-alpha

    Plasmid

    #109791

    Purpose

    Protein expression and purification of GNAI2_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNAI2_G-alpha (GNAI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM33_MDM2_SWIB

    Plasmid

    #178481

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    MDM2_SWIB (MDM2 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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