30,535 results
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Plasmid#83959PurposeContains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.DepositorInsertInternal Control sequence
UseTagsExpressionBacterialMutationPromoterAvailable SinceNov. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
spNW25
Plasmid#173484PurposeExpresses spNW25 in Bl21DepositorInsertspNW25
UseTagsHistagExpressionBacterialMutationPromoterAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pPSU20-40-60-80
Plasmid#173839PurposeCoexpresses 20, 40, 60 and 80 kD Penn State ladder proteinsDepositorInsertSTRHSTPABPACCBPv3
UseTagsExpressionBacterialMutationPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E3D1
Plasmid#20066PurposeBacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formationDepositorInsertMSP1E3D1
UseTags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeats of …PromoterAvailable SinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
His6-pAG-RTase
Plasmid#214932PurposeExpress fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systemsDepositorInsertfusion protein of protein A/G and MMLV reverse transcriptase (25-497)
UseTagsHis6ExpressionBacterialMutationMMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…PromoterT7 promoterAvailable SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-GFP-Nanobody
Plasmid#61838PurposeExpresses GST-tagged anti-GFP Nanobody in E. coli cells.DepositorInsertGFP-Nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1
Plasmid#20061PurposeBacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formationDepositorInsertMSP1D1
UseTags7-His tagExpressionBacterialMutationdeletion mutant (1-11) of MSP1; His-tag on N-term…PromoterAvailable SinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTP1122
Plasmid#104159PurposeBacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)DepositorInsertAnti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pExp-MBP
Plasmid#112568PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-MBP and Strep tagExpressionBacterialMutationPromoterAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-NusA
Plasmid#112571PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8His and Strep tagExpressionBacterialMutationPromoterAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
AFDN_PDZ_1
Plasmid#103938PurposeProtein expression and purification of TRITHORAX PDZ domainDepositorInsertAFDN (AFDN Human)
UseTagsGSTExpressionBacterialMutationcontains AA983-1110 of AFDNPromotertacAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB-Rex
Plasmid#122134PurposeB. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 originDepositorInsertB. subtilis Rex
UseTagsExpressionBacterialMutationPromoterapFAB338Available SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAL-SH3(2)-5R
Plasmid#112089PurposeBacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.DepositorInsertSH3(2)-5R (NCK1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationconstruct contains only repeats of the second SH3…PromoterLacAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)
Plasmid#177850PurposeBacterial Expression of AMPKDepositorInsertAMPK (a1b2g1)
UseTagsMBPExpressionBacterialMutationPromoterAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMal-Abl-PRM 5R
Plasmid#112088PurposeBacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.DepositorInsertPRM-5R (ABL1 Human)
UseTagsHis-6 and MBPExpressionBacterialMutationconstruct contains only the PRM motif of Abl1PromoterLacAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)10
Plasmid#126948PurposeBacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.DepositorInsert10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationPromoterunknownAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pROP-PP-FBFP
Plasmid#122137PurposeFMN-based fluorescent protein Reporter plasmid for B-Rex contain perfect operator sequence (anaerobic purpose)DepositorInsertFBFP
UseTagsExpressionBacterialMutationPromoterJ23101Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
(SIM)10
Plasmid#126946PurposeBacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.DepositorInsert10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
UseTagsExpressionBacterialMutationPromoterunknownAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pROP-PP-GFP
Plasmid#122135PurposeGFP Reporter plasmid for B-Rex contain perfect operator sequenceDepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterJ23101Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only