31,059 results
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Plasmid#134879PurposeBacterial expressionDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialMutationdeletion of Y208Available SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-ffDronpa
Plasmid#78182PurposeExpresses ffDronpa E. coli strainsDepositorInsertffDronpa
Tags6xHis-tag; Xpress tagExpressionBacterialPromoterT7Available SinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension
Plasmid#176542PurposeBacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locusDepositorInsertsMBP tag
TnpB
Tags3' extension derived from endogenous locus a…ExpressionBacterialAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X1 Flag RbBP5
Plasmid#15551DepositorAvailable SinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP6 (catalytic domain)
Plasmid#16359DepositorInserthuman SENP6 catalytic domain (SENP6 Human)
TagsHisExpressionBacterialMutationAmino Acid 628 to 1112 catalytic domain of human …Available SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTlpA-Wasabi-NF
Plasmid#86100PurposeNonfluorescent variant of mWasabi reporter gene gated by wild type TlpADepositorInsertsTlpA
mWasabi
Tags6xHisExpressionBacterialMutationD341A and S71T, G73APromoterTlpA and TlpA, LacIAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPEP1
Plasmid#61046PurposeBglFusion compatible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (5B)
Plasmid#85499PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
cr3Ec
Plasmid#184860Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination systemDepositorInsertCas9, gam, bet, exo
TagsCas9 ssrA degradation tagExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a_SMOXdel
Plasmid#186785PurposeC-terminal His-Tag and a TEV cleaveage site, deletion of 8 residues (503-512) to transform into E. coli BL21 (DE3) cellsDepositorInsertSpermine oxidase (SMOX Human)
TagsHis tagExpressionBacterialMutationdeleted amino acids 503-512Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHelper_TetR/pTet-Tnpase (carbR)
Plasmid#213911PurposeATc-inducible himar1C9 transposase expression - carb/amp resistanceDepositorInsertTetR/ptet ATc-inducible himar1C9 transposase
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn[sfGFP]
Plasmid#213902PurposeMariner transposon expressing sfGFP with kan selection casetteDepositorInsertmariner transposon with kan resistance and superfolder GFP
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB1-ALIX(P801G)
Plasmid#180025PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.DepositorInsertGB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)
TagsGB1-His6-TEVExpressionBacterialMutationP801GAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJJS005
Plasmid#188328PurposePCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::mNeonGreen::Linker
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPSA5Cas9syn2
Plasmid#206963PurposeS. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGATDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b
Plasmid#184565PurposeInducible expression of PbuCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Prevotella buccae
ExpressionBacterialPromoterT7 promoterAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEB2-2ndVal mCherry2-L
Plasmid#104027PurposePlasmid encoding 2ndVal mCherry2-LDepositorInsert2ndVal mCherry2-L
UseLow copyExpressionBacterialMutationmCherry2-L with valine inserted in 2nd positionPromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAB168
Plasmid#101671PurposeOpto-T7RNAP*(563-F1), equal expr.DepositorInsertOpto-T7RNAP*(563-F1), equal expr.
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-CoilR-Cys
Plasmid#181989PurposeUsed to label proteins with the CoilE genetically encoded tagDepositorInsertCoilR-Cys
TagsHis-TagExpressionBacterialAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAKgfplux2
Plasmid#14084DepositorInsertBacterial luciferase-lacIq repressor gene
ExpressionBacterialAvailable SinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His
Plasmid#171587PurposeBacterial expression of NLS-RfxCas13d-NLS 6HisDepositorInsertMKIEE-NLS-RfxCas13d-NLS-HA-6His
TagsHA-6HIS and MKIEEExpressionBacterialAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pE5c
Plasmid#17465Purposemodified pENTR vector for C-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherry-2xEcMTS
Plasmid#133624PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherry-2xEcMTS
TagsHisExpressionBacterialAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mEGFP-KanMX6
Plasmid#87023PurposeTag S. pombe genes in C terminal with mEGFPDepositorInsertmEGFP
ExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
5H9M (SIAH2)
Plasmid#79521PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 15, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
KLHL3 (KLHL3A-c003)
Plasmid#110251PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZmUbi-5'UTR
Plasmid#154057PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)DepositorInsertPromoter and 5' UTR Ubiquitin (Zea mays)
UseSynthetic BiologyExpressionBacterialPromoterN/AAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-A4DARPin
Plasmid#198288PurposeProduces PVCs retargeted with anti-lysozyme DARPin A4DepositorInsertPVCpnf_pvc13-A4DARPin
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLP-pR-himar-apraR
Plasmid#199996PurposeLanding Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donorDepositorInsertLanding Pads
ExpressionBacterialAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
RAB25
Plasmid#26163PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTBXh-2
Plasmid#26078DepositorTypeEmpty backboneTagsHisExpressionBacterialAvailable SinceNov. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
B7-5
Plasmid#111493Purposetranslational initiation factor 1DepositorInserttranslational initiation factor 1
Tags6xHisExpressionBacterialAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOT2
Plasmid#14460DepositorInsertGfp-UV
ExpressionBacterialAvailable SinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFPV25
Plasmid#20667DepositorTypeEmpty backboneTagsGFP mut3ExpressionBacterialAvailable SinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pENTR R4 ATCC LacZ GCTT
Plasmid#173668PurposeDestination vector for Golden Gate Cloning assemblies, blue-white screeningDepositorTypeEmpty backboneExpressionBacterial and InsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKPY514
Plasmid#62598PurposeEncodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) ControlDepositorInsertThr251Gly-EcPheRS
Tags6xHisExpressionBacterialMutationThr251GlyPromoterT5Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
RALA
Plasmid#55657Purposeexpression of mouse RALADepositorAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPd_PepTSt
Plasmid#58333PurposeExpresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged proteinDepositorInsertPepTSt
TagsGFP-HisExpressionBacterialPromoterT7Available SinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pAN3944
Plasmid#74702PurposeCircuit plasmid: 0x1CDepositorInsertCircuit 0x1C
ExpressionBacterialAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only