32,537 results
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Plasmid#175272PurposeLactate biosensor in anaerobic conditionsDepositorInsertLldR_Synthetic_promoter_sfGFP
ExpressionBacterialAvailable SinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
psfTn5
Plasmid#79107Purposebacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutationsDepositorInserttransposase 5
TagsHisExpressionBacterialMutationE54K and L372PAvailable SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-TEV-C9R
Plasmid#118754PurposeTEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activityDepositorInsertTobacco Etch Virus protease (NEWENTRY Synthetic)
TagsC9R and His tagExpressionBacterialPromoterT7Available SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
p27-mVenus reporter (PB)
Plasmid#190731PurposeThe p27 reporter vector, which expresses mVenus-fused, mutant (K–) p27 protein with a defective CDK binding.DepositorInsertsmVenus
p27
ExpressionBacterial and MammalianMutationp27-F62A-F64APromoterEF1αAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFW2000
Plasmid#224211PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-MacroGreen
Plasmid#160665PurposeExpresses GFP fusion of a multisite mutant of the Af1521 proteinDepositorInsertAf1521 modified synthetic construct eGFP fusion
TagseGFP and hexahistidine_TEV recognitionExpressionBacterialPromoterLacIAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUMO3
Plasmid#52260PurposeBacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2DepositorTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a hMDH2
Plasmid#204230PurposeBacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).InserthMDH2 (MDH2 Human)
Tags6X HisExpressionBacterialMutationDoes not include transit peptide and includes a M…PromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
poRBS
Plasmid#154125PurposeExpresses orthogonal rRNAs operon in bacterial cellsDepositorInsertengineered bacterial rRNAs operon under control of pL promotor
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas12a-NT
Plasmid#235167PurposeExpresses the Wild Type Francisella novicida cas12a gene and a NT crRNADepositorInsertFnCas12a
ExpressionBacterialAvailable SinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-human p53-(1-393)
Plasmid#24860DepositorAvailable SinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
GST-beta-arrestin1
Plasmid#36918DepositorAvailable SinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_HaloTag9
Plasmid#169324PurposeExpression of HaloTag9 in bacterial cellsDepositorInsertHaloTag9
TagsHisExpressionBacterialMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-EcMscL-TEV-mEGFP
Plasmid#165097PurposeExpresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.DepositorInsertE. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
UseSynthetic BiologyTagsmonomeric Enhanced Green Fluorescent ProteinExpressionBacterialPromoterT7Available SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Reb_1 aka PAS619
Plasmid#71224PurposeExpresses wild type R bodies from C. taeniospiralis in pETM11 backboneDepositorInsertReb locus
TagsnoneExpressionBacterialMutationRebA G53APromoterT7Available SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFnCas12a_NT
Plasmid#169838PurposeExpresses FnCas12a and guide RNA in BacteriaDepositorInsertFnCas12a
UseCRISPRExpressionBacterialPromoterpXynAAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB082a
Plasmid#79233PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d123
ExpressionBacterialPromoterPpro1Available SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF1-Pls4-sfGFP
Plasmid#205751PurposeExpresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.DepositorTypeEmpty backboneUseShuttle vector gram+ gram-ExpressionBacterialPromoterPls4 (Phyperspank mutant, IPTG inducible)Available SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSR1058
Plasmid#208743PurposeCharacterization plasmid for PHbs (sfgfp expressed from PHbs on the S. aureus pC194 replicon)DepositorInsertPHbs
UseSynthetic BiologyExpressionBacterialPromoterPHbsAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)
Plasmid#167268PurposeOverexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coliDepositorInsertHOB
TagsHis x10ExpressionBacterialPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only