30,904 results
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Plasmid#222930PurposeShuttle vector carrying a Candida auris centromere (CEN 4) with Nourseothricin selectable markerDepositorInsertCEN 4
ExpressionBacterialAvailable SinceJuly 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET11a-p52(1-398)
Plasmid#44733DepositorAvailable SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
PHYHD1
Plasmid#38854PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFGET19_Ulp1
Plasmid#64697PurposeH6-Ulp1 from pHYRS52 into pET-28b(+)DepositorInsertULP1 (ULP1 Saccharomyces cerevisiae, Budding Yeast)
Tags6xHisExpressionBacterialMutationUlp1(403-621)PromoterT7Available SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBR1MCS-2
Plasmid#85168PurposeMobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneExpressionBacterialPromoterConstitutive (derepressed P-lac)Available SinceNov. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
MP6
Plasmid#69669PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi CDA1
ExpressionBacterialPromoterE. coli PBADAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET29b-10xHis-Super TEV
Plasmid#193833PurposeExpresses high-efficiency TEV protease in bacterial cellsInsertTEV protease
Tags6xHisExpressionBacterialMutationT17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…Available SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-6xTR-TUBE
Plasmid#110313PurposeE. coli expression and purification of 6xTR-TUBEDepositorInsert6xTR-TUBE (UBQLN1 Human)
TagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF
Plasmid#31186PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAGDepositorInsertM.j. p-azidohenylalanine RS (2 copies +tRNA)
ExpressionBacterialMutationY32T, E107N, D158P, I159L, L162Q, D286RAvailable SinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
TurboID-His6_pET21a
Plasmid#107177Purposeexpresses His6-tagged TurboID in bacteriaDepositorInsertTurboID (BirA mutant)
ExpressionBacterialMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK793
Plasmid#8827DepositorInsertTEV protease, S219V mutant
TagsHis, MBP (with TEV), and polyarginineExpressionBacterialMutationS219V mutation (improves stability)Available SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJC175e
Plasmid#79219PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPPSP gIII, luxAB
ExpressionBacterialPromoterPPSPAvailable SinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKL2299A
Plasmid#222105PurposeA ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.DepositorInsertvirA
ExpressionBacterial and PlantPromotervirA promoterAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC013 - Twinstrep-SUMO-huLwCas13a
Plasmid#90097PurposeTwinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.DepositorInsertLwCas13a
UseCRISPRTags6xHis-Twin Strep-SUMOExpressionBacterialAvailable SinceJune 13, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-pAG-Hia5
Plasmid#174373PurposePlasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertprotein AG - hia5
Tags6xHis and Protein A/GExpressionBacterialAvailable SinceSept. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody
Plasmid#199390PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tagDepositorInsertanti-ALFA nanobody
Tags14xHis-Avi-SUMOEuExpressionBacterialPromoterT5-LacOAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTn Donor
Plasmid#236211PurposeArabinose-inducible Tn5 InducTn-seq plasmidDepositorInsertNone
ExpressionBacterialAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG/MNase
Plasmid#123461PurposeExpresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.DepositorInsertProtein A-Protein G-Micrococcal Nuclease
Tags6xHIS and HAExpressionBacterialPromoterLacAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
DP6
Plasmid#140446PurposeUntargetted bacterial driftDepositorInsertdnaQ926 dam seqA emrR ugi CDA1 gIII
ExpressionBacterialAvailable SinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP
Plasmid#102634PurposeIn vitro expression of sfGFP from the T7 promoterDepositorInsertsfGFP
Tagsstrep tagExpressionBacterialPromoterT7Available SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only