30,296 results

1. pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

   Plasmid

   #199371

   Purpose

   Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   anti-ALFA nanobody
   

   Use

   Tags

   14xHis-Avi

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T5-LacO

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6P1-GFP-Nanobody

   Plasmid

   #61838

   Purpose

   Expresses GST-tagged anti-GFP Nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

   Insert

   GFP-Nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-28a_6H-MMLV_RT_D524N-6H

   Plasmid

   #166945

   Purpose

   Bacterial expression of M-MLV reverse transcriptase

   Depositor

   Robert Tjian

   Article

   Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

   Insert

   gag-pol
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   D524N, H8Y

   Promoter

   T7

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEVOL-pAzF

   Plasmid

   #31186

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

   Depositor

   Peter Schultz

   Article

   Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

   Insert

   M.j. p-azidohenylalanine RS (2 copies +tRNA)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32T, E107N, D158P, I159L, L162Q, D286R

   Promoter

   Available since

   Dec. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDisCoTune

   Plasmid

   #173713

   Purpose

   Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

   Depositor

   Morten Norholm

   Article

   Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

   Inserts

   ERV1 (ERV1 Budding Yeast)
   PDI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

   Plasmid

   #199390

   Purpose

   Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   anti-ALFA nanobody
   

   Use

   Tags

   14xHis-Avi-SUMOEu

   Expression

   Bacterial

   Mutation

   Promoter

   T5-LacO

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBeloBac11loxP2272

   Plasmid

   #60342

   Purpose

   This is a BAC vector that contains both wt and mutant loxP sites.

   Depositor

   Jon Coren

   Article

   Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACYC-GroEL/ES-TF

   Plasmid

   #83923

   Purpose

   Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Gro EL/ES
   Trigger Factor
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDB179-HotPETase

   Plasmid

   #218701

   Purpose

   Encodes codon optimized HotPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   HotPETase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-sfGFP 150TAG

    Plasmid

    #85493

    Purpose

    pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP 150TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    150 TAG (amino acid 150 mutated to stop codon)

    Promoter

    T7

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28:GFP

    Plasmid

    #60733

    Purpose

    pET28 driving expression of GFP, biobrick part Bba_E0040

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX6P1-N-His

    Plasmid

    #119755

    Purpose

    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.

    Depositor

    Andrew Jackson, Martin Reijns

    Type

    Empty backbone

    Use

    Tags

    10xHis and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. mEGFP-NbALFA

    Plasmid

    #159986

    Purpose

    For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    mEGFP-NbALFA
    

    Use

    Tags

    8histidine-HRV3C protease cleavage site

    Expression

    Bacterial

    Mutation

    3GS linker between mEGFP and NbALFA

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. plRL19

    Plasmid

    #163634

    Purpose

    L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Insert

    L5 Integrase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mycobacterial Optimized Promoter (MOP) and Tet-On

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. plRL58

    Plasmid

    #166886

    Purpose

    Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTP1122

    Plasmid

    #104159

    Purpose

    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-MBP-mSA2

    Plasmid

    #52319

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Use

    Tags

    FLAG, His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD-mTagBFP2

    Plasmid

    #34632

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I174A compared to mTagBFP (enhanced photostabilit…

    Promoter

    Available since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET3a-hUBA1

    Plasmid

    #63571

    Purpose

    Expression of human UBA1 tagged with His

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBA1 (UBA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Isoform 2 (1018 amino acids)

    Promoter

    T7

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge