30,738 results

1. pCRISPR

   Plasmid

   #42875

   Purpose

   A crRNA expression plasmid for targeting a specific sequence.

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   CRISPR-BsaI
   

   Use

   CRISPR; E.coli

   Expression

   Bacterial

   Available Since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a-BirA

   Plasmid

   #20857

   Depositor

   Alice Ting

   Article

   Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

   Insert

   BirA
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACYC-GroEL/ES-TF

   Plasmid

   #83923

   Purpose

   Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Gro EL/ES
   Trigger Factor
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pREDawn-AmpR-MCS

   Plasmid

   #188978

   Purpose

   For red light-induced bacterial expression of gene of interest (AmpR)

   Depositor

   Heikki Takala

   Article

   Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

   Plasmid

   #198468

   Purpose

   E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

   Depositor

   Viviana Risca

   Article

   Soroczynski et al bioRxiv 2024.07.11.602973

   Insert

   10His-pG-Tn5 (E54K, L372P)
   

   Tags

   10His-pG

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   Consensus φ10 T7 Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMJ915

   Plasmid

   #69090

   Purpose

   Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

   Depositor

   Jennifer Doudna

   Article

   Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

   Insert

   cas9
   

   Tags

   MBP, hexa His, and two SV40 NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pON.mCherry

   Plasmid

   #84821

   Purpose

   Constitutive Expression plasmid with mCherry fluorescent protein

   Depositor

   Howard Shuman

   Article

   Gebhardt et al PLoS One. 2017 Mar 3;12(3):e0173116. doi

   Insert

   mCherry
   

   Expression

   Bacterial

   Promoter

   Tac Promoter

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

   Plasmid

   #107581

   Purpose

   B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

   Depositor

   Paul Freemont

   Article

   Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pdh promoter Bacillus megaterium DSM319

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL-MS2-12X

   Plasmid

   #27119

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   MS2 binding sites (12)
   

   Expression

   Bacterial

   Available Since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET StrepII TEV LIC cloning vector (1R)

    Plasmid

    #29664

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    Strep II and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSL-MS2-6X

    Plasmid

    #27118

    Depositor

    Robert Singer

    Article

    Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

    Insert

    MS2 binding sites (6)
    

    Expression

    Bacterial

    Available Since

    Dec. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. S96 ELP

    Plasmid

    #68393

    Purpose

    Elastin-like polypeptide (VPGxG) with 96 repeats of Serine guest residue

    Depositor

    Andrew MacKay

    Article

    Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

    Insert

    S96 ELP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. V96 ELP

    Plasmid

    #68392

    Purpose

    Elastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residue

    Depositor

    Andrew MacKay

    Article

    Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

    Insert

    V96 ELP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pComb8

    Plasmid

    #63889

    Purpose

    For phage display of antibody libraries by fusion to modified geneVIII.

    Depositor

    Carlos Barbas

    Article

    Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

    Type

    Empty backbone

    Use

    Phage display

    Tags

    geneVIII

    Expression

    Bacterial

    Promoter

    lacZ

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pREDusk-MCS

    Plasmid

    #188970

    Purpose

    For red light-repressed bacterial expression of gene of interest

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYTK068

    Plasmid

    #65175

    Purpose

    Encodes ConR2 as a Type 5 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    ConR2
    

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pet28a-His6-Keap1

    Plasmid

    #62454

    Purpose

    His6 tagged Keap1 for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Tags

    His-Tag

    Expression

    Bacterial

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. p300dAIL KAT LIC 1M

    Plasmid

    #233588

    Purpose

    Bacterial expression of p300 KAT enzyme with truncated autoinhibitory loop

    Depositor

    Scott Showalter

    Article

    Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

    Insert

    p300dAIL KAT (EP300 Human)
    

    Tags

    6xHis MBP

    Expression

    Bacterial

    Mutation

    deleted amino acids 1523-1554

    Promoter

    T7

    Available Since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYTK095

    Plasmid

    #65202

    Purpose

    Encodes AmpR-ColE1 as a Type 678 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYTK067

    Plasmid

    #65174

    Purpose

    Encodes ConR1 as a Type 5 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    ConR1
    

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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