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pD864-LacZ

Plasmid

#109380

Purpose

Rhamnose inducible LacZ expression. Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pRHAM-LacZ

Expression

Bacterial

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSR33.4r

Plasmid

#63198

Purpose

Expresses Cph8, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

Cph8
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pXD68Kan2-AphA

Plasmid

#191248

Purpose

RSF1010-based broad host-range plasmid engineered from pAM5409, kanamycin resistance

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMAL-7xHis-TEV-hexa

Plasmid

#222868

Purpose

Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

Depositor

Nikolaus Loening

Article

Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

Insert

TEV protease

Tags

7xHis and MBP

Expression

Bacterial

Mutation

C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pComb3XSS

Plasmid

#63890

Purpose

3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment

Depositor

Carlos Barbas

Article

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Inserts

SS Stuffer
Light Chain Stuffer
Heavy Chain Stuffer

Use

Phage display

Tags

6x His, HA tag, and geneIII

Expression

Bacterial

Promoter

lacZ

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pTargetF

Plasmid

#62226

Purpose

Constitutive expression of sgRNA without donor editing template DNA

Depositor

Sheng Yang

Article

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Insert

sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pij23119

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCI-neo-cOVA

Plasmid

#25097

Depositor

Maria Castro

Article

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

cytoplasmic ovalbumin

Expression

Bacterial and Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pFPV-mCherry

Plasmid

#20956

Depositor

Olivia Steele-Mortimer

Article

Drecktrah et al Traffic. 2008 Dec . 9(12):2117-29.

Insert

mCherry

Expression

Bacterial

Available Since

June 3, 2009

Availability

Academic Institutions and Nonprofits only

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pTET GFP10-FRB/FKBP-GFP11

Plasmid

#182497

Purpose

Tripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coils

Depositor

Stéphanie Cabantous

Article

Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

Inserts

FRB
FKBP (Fkbp1a Mouse, Synthetic)

Tags

split GFP10 tripartite and split GFP11 tripartite

Expression

Bacterial

Promoter

TET

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAM5411

Plasmid

#132663

Purpose

RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Inserts

aadA
YFP

Expression

Bacterial

Promoter

PaadA and PconII

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pRBC-sfGFP-150TAG

Plasmid

#174076

Purpose

Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

Depositor

Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Insert

Superfolder GFP

Tags

His-6

Expression

Bacterial

Mutation

N150TAG

Promoter

T7

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP

Plasmid

#102634

Purpose

In vitro expression of sfGFP from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

sfGFP

Tags

strep tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGM190

Plasmid

#69614

Purpose

inducible gene expression in Streptomyces

Depositor

Günther Muth

Article

Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

Inserts

aphII
tsr
rep
sso
TipA promoter
dso

Use

Streptomyces- e. coli shuttle vector

Expression

Bacterial

Available Since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET-6xTR-TUBE

Plasmid

#110313

Purpose

E. coli expression and purification of 6xTR-TUBE

Depositor

Yasushi Saeki

Article

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

6xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pQE30-His-CKM

Plasmid

#124134

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Creatine kinase (CKM Chicken)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-TGFB1

Plasmid

#120361

Purpose

Produce human recombinant TGFB1 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Transforming Growth Factor-β1 (TGFB1 Human, Synthetic)

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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eSrtA(2A-9) pET29b

Plasmid

#75145

Purpose

reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

Depositor

David Liu

Article

Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

reprogrammed sortase A variant

Tags

6x His

Expression

Bacterial

Mutation

S102C A104H E105D K138P K152I N160K K162H T164N K…

Promoter

T7

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only

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pRAM18dRGA[MCS]

Plasmid

#84683

Purpose

Can be used for the transformation of rickettsia

Depositor

Ulrike Munderloh

Article

Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19 Giles integrase

Plasmid

#170049

Purpose

pUC19 with Giles integrase

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Insert

GIles Integrase

Expression

Bacterial

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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SPYtag-Histag-Avidin

Plasmid

#214836

Purpose

This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

SPYtag-Histag-Avidin

Expression

Bacterial

Promoter

T7

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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