30,607 results

1. pET29b-10xHis-Super TEV

   Plasmid

   #193833

   Purpose

   Expresses high-efficiency TEV protease in bacterial cells

   Depositor

   Florence Pojer, EPFL Protein Production and Structure Core Facility (PTPSP)

   Article

   Lau et al protocols.io, doi: 10.17504/protocols.io

   Insert

   TEV protease
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

   Promoter

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPSU10-30-50-100

   Plasmid

   #173838

   Purpose

   Coexpresses 10, 30, 50 and 100 kD Penn State ladder proteins

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   STRHSTPAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPSU20-40-60-80

   Plasmid

   #173839

   Purpose

   Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   STRHSTPABPACCBPv3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC18T-mini-Tn7T-Tp-gfpmut3

   Plasmid

   #65033

   Purpose

   TpR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   gfpmut3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

   Plasmid

   #212829

   Purpose

   Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

   Depositor

   Wolfgang Enard

   Article

   Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

   Insert

   ZIM3-KRAB-dCas9-P2A-mCherry
   

   Use

   Tags

   HA-tag and ZIM3-KRAB

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG, TetOn

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEVOL-pAzF

   Plasmid

   #31186

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

   Depositor

   Peter Schultz

   Article

   Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

   Insert

   M.j. p-azidohenylalanine RS (2 copies +tRNA)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32T, E107N, D158P, I159L, L162Q, D286R

   Promoter

   Available Since

   Dec. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xFLAG-CUL4A-pCMV7.1

   Plasmid

   #155022

   Purpose

   N-terminal 3xFLAG-tagged CUL4A in a pCMV7.1 vector

   Depositor

   Andre Catic

   Article

   Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

   Insert

   Cullin 4A (CUL4A Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pCMV7.1

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpyCatcher003-sfGFP

   Plasmid

   #133449

   Purpose

   Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003-sfGFP
   

   Use

   Tags

   His6 and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET29a-human histone 4-in-One

   Plasmid

   #223237

   Purpose

   Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

   Depositor

   Weixing Zhao

   Inserts

   His6-VSVG-Histone H2A
   Histone H2B
   Histone H3
   Histone H4-TEV-His6-Dual StrepII
   

   Use

   Tags

   Dual StrepII, His6, TEV protease site, and VSVG

   Expression

   Bacterial

   Mutation

   A103G

   Promoter

   T7

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFNR5

    Plasmid

    #149483

    Purpose

    Broad-Host range expression vector, RSF1010 origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    NahR(AM)-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PSalTTC

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21a-BirA

    Plasmid

    #20857

    Depositor

    Alice Ting

    Article

    Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    BirA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZP06C

    Plasmid

    #232322

    Purpose

    pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.

    Depositor

    Chenggang Wu

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsJ

    Available Since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAV1K-T5-gfp

    Plasmid

    #26702

    Depositor

    Ichiro Matsumura

    Article

    Bryksin et al PLoS One. 2010 . 5(10):e13244.

    Insert

    T5lacOlacOeGFP biobrick
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET29b_1_anti-GFP-nanobody

    Plasmid

    #210394

    Purpose

    Express anti-GFP nanobody in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    1_anti-GFP-nanobody
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pBWB162

    Plasmid

    #140634

    Purpose

    pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-BiFC

    Plasmid

    #87856

    Purpose

    pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Inserts

    Encodes for MCS & C-term half of mVenus
    Encodes for MCS & N-term half of mVenus
    

    Use

    Tags

    BiFC C-terminal fragment

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ubiquitin WT

    Plasmid

    #12647

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    Ubiquitin - wild type (UBC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. prhaBAD-anti-rabbit-Nb-Hia5

    Plasmid

    #239627

    Purpose

    Rhamnose inducible expression plasmid for anti-rabbit nanobody fusion to Hia5 DNA adenine methyltransferase

    Depositor

    Aaron Straight

    Article

    Sidhwani et al bioRxiv 2025.07.01.662447; doi: https://

    Insert

    Hia5
    

    Use

    Tags

    6HIS, MBP, TEV, and anti-Rabbit Nanobody

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Promoter

    rhaBAD

    Available Since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-RT003-GPM

    Plasmid

    #163383

    Purpose

    To express sGFP module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    GFP-PDGFRtm-2A-mCherry (GPM)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQE-60 Grx1-roGFP2-His

    Plasmid

    #64799

    Purpose

    Bacterial expression of His-tagged Grx1-roGFP2

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Tags

    His and roGFP2

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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