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32,656 results

Showing: 21 - 40 of 32656 results

  4. pSecUAG-Evol2

    Plasmid
    #163148
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons
    Depositor
    Dieter Söll
    Article
    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2
    Inserts
    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG), Cys…
    Promoter
    EM7, araBAD, native As SelD promoter, and native …
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    163148-plasmid-map-sequence-id-331409

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  6. pAX2

    Plasmid
    #117398
    Purpose
    allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch
    Depositor
    Karen Guillemin
    Article
    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    117398-plasmid-map-sequence-id-230535

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  11. pSLTS

    Plasmid
    #59386
    Purpose
    Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.
    Depositor
    Shelley Copley
    Article
    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.
    Type
    Empty backbone
    Use
    Genome engineering
    Expression
    Bacterial
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59386-plasmid-map-sequence-id-380750

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  13. pT22-GluCNZ-6His

    Plasmid
    #213504
    Purpose
    Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.
    Depositor
    John Denu
    Article
    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12
    Insert
    Glucanase (glucan endo-1,3-beta-glucosidase)
    Tags
    6His tag
    Expression
    Bacterial
    Mutation
    1-36 Signal Sequence Deletion
    Promoter
    T7
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213504-plasmid-map-sequence-id-424932

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  15. pSCM001

    Plasmid
    #124605
    Purpose
    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH
    Depositor
    David Summers
    Article
    Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1
    Insert
    mCherry-pHluorin
    Tags
    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…
    Expression
    Bacterial
    Promoter
    pDAB
    Available Since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    124605-plasmid-map-sequence-id-242761

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Showing: 21 - 40 of 32656 results