31,188 results
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Plasmid#11354PurposeLuciferase reporter containing three copies of vitellogenin Estrogen Response ElementDepositorInsertERE
UseLuciferaseExpressionBacterialAvailable SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1(nsp8-nsp7)(nsp12)
Plasmid#165451PurposeExpresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia ColiDepositorInsertsnsp12
nsp8
nsp7
Tags14Histidine-TEVExpressionBacterialPromoterT7Available SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas9-recX
Plasmid#158715PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recXDepositorInsertcas9
UseCRISPRExpressionBacterialPromoterTetOAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBB540
Plasmid#27393DepositorInsertgrpE, clpB
ExpressionBacterialAvailable SinceApril 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
tFucci(SA)5
Plasmid#153520PurposeNew color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.DepositorInserttFucci(SA)5.
TagsAzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)ExpressionBacterial and MammalianPromoterCAGAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR
Plasmid#42875PurposeA crRNA expression plasmid for targeting a specific sequence.DepositorInsertCRISPR-BsaI
UseCRISPR; E.coliExpressionBacterialAvailable SinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Beta-Lactamase
Plasmid#62729PurposeExpresses Beta-Lactamase protein in bacterial cellsDepositorInsertBeta-Lactamase
TagsHis tagExpressionBacterialMutationDeletion of periplasmic signal domainPromoterT7Available SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT85_PS-mEos3.2_GentaR
Plasmid#173895PurposeTransposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoterDepositorInsertmEos3.2
ExpressionBacterialPromoterSpiralinAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
HP4_M13
Plasmid#120340PurposeHelper plasmid for production of phagemid ssDNA, based on m13mp18DepositorInsertCoding part of the M13 phage genome
ExpressionBacterialPromoternative phage promotersAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pULTRA-CNF
Plasmid#48215Purposehighly efficient suppressor plasmidDepositorInsertspolyspecific MJ tyrosyl synthetase
tRNA
ExpressionBacterialMutationY32L, L65V, F108W, Q109M, D158G, I159APromoterproK and tacIAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
His6-MBP-uTEV3
Plasmid#135464PurposeBacterial expression of uTEV3 (full-length) proteaseDepositorInsertHis6-MBP-uTEV3
TagsMBP, His6ExpressionBacterialMutationS219V mutation (improves stability) , I138T/S153N…PromoterT5Available SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHyperTEV60
Plasmid#228496Purpose10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)DepositorInsertTEV Protease
Tags10xHis-SUMO(Smt3)ExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIDA1
Plasmid#79180PurposeA construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tagsDepositorInsertAdhesin Involved in Diffuse Adherence
TagsHis and MycExpressionBacterialPromoterlacUV5Available SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-TACC3-His6
Plasmid#69106PurposeFor bacterial expression of recombinant human TACC3 tagged with GST and His.DepositorInsertTransforming Acidic Coiled Coil protein 3 (TACC3 Human)
TagsGST and His6ExpressionBacterialAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZP06C
Plasmid#232322PurposepZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.DepositorInsertmCherry
ExpressionBacterialPromoterrpsJAvailable SinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWUR790
Plasmid#101723PurposeSynthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgoDepositorInsertPfAgo
TagsStrep(II)-tagExpressionBacterialPromoterT7 PromotorAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCF1135
Plasmid#222106PurposeE. coli - Veillonella shuttle plasmid with single selectable markerDepositorTypeEmpty backboneUseE. coli-veillonella shuttle plasmidExpressionBacterialAvailable SinceOct. 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAC-lacZ
Plasmid#13422DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/LoxExpressionBacterialAvailable SinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBB542
Plasmid#27395DepositorInsertdnaK, dnaJ, groESL
ExpressionBacterialAvailable SinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBB541
Plasmid#27394DepositorAvailable SinceMarch 13, 2012AvailabilityAcademic Institutions and Nonprofits only