32,052 results

Showing: 21 - 40 of 32052 results

Results per page:

Sort results by:

pMTTH+H1.4

Plasmid

#157796

Purpose

Expression of human linker histone H1.4

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H1.4 (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pOlyA(WT)

Plasmid

#132391

Purpose

Ostreolysin A for sensing sphingomyelin/cholesterol complexes

Depositor

Arun Radhakrishnan

Article

Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

Insert

Ostreolysin A wild-type

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: C62S, C94S, S151C

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Article

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mKate1.31-pBAD

Plasmid

#54827

Purpose

Localization: Protein Expression Vector , Excitation: 588, Emission: 635. Also known as mKate2.

Depositor

Michael Davidson

Article

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Type

Empty backbone

Tags

6xHis and mKate1.31 (aka mKate2)

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
evoFERNY-Cas9NG

Plasmid

#203329

Purpose

Plasmid for bacterial purification of codon optimized evoFERNY-Cas9NG

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

evoFERNY-Cas9NG

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS4333 yeTadA1.0-T7 RNAP

Plasmid

#137735

Purpose

pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

Depositor

Christina Smolke

Article

Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

Insert

yeTadA1.0-T7 RNAP

Expression

Bacterial and Yeast

Mutation

E366G (See depositor comment below)

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-17X-PTDH

Plasmid

#166786

Purpose

Phosphite dehydrogenase for NAD(P)H regeneration

Depositor

Wilfred van der Donk

Article

Yang et al J Am Chem Soc. 2015 Oct 7;137(39):12426-

Insert

Phosphite dehydroenase

Tags

His6

Expression

Bacterial

Mutation

A196R, T201S, A328T, E352N, and C356D- please see…

Promoter

T7

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA221pmCDA1-T7RNAP

Plasmid

#167978

Purpose

Expresses fusion protein pmCDA1-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

pmCDA1-T7RNAP (T7p07 Synthetic)

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PA-Cdc42

Plasmid

#75263

Purpose

Light-activated Cdc42

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

Cell Division Cycle 42 (CDC42 Human)

Tags

6xHIs, LOV2, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

F56W

Promoter

CMV

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
HSA2_His

Plasmid

#202764

Purpose

human serum albumin design HSA2, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA2

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-26b-Nb127D01-ALFA-His

Plasmid

#171568

Purpose

Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-ALFA-His (CXCR2 Human)

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKESK22

Plasmid

#64857

Purpose

expression vector, low to medium copy number, tac promoter lacI, kanamycin resistance, p15A origin of replication

Depositor

Karin Schnetz

Article

Stratmann et al J Bacteriol. 2008 Feb;190(3):926-35. Epu

Type

Empty backbone

Expression

Bacterial

Promoter

Ptac

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX TIF1gamma

Plasmid

#15734

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

TIF1 gamma (TRIM33 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
GFP-(SUMO)1

Plasmid

#127089

Purpose

Bacterial expression plasmid for eGFP-(SUMO)1 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP human SUMO3 (SUMO3 Human)

Tags

EGFP

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDC180

Plasmid

#218673

Purpose

Expresses rat ⍺SNAP in bacteria

Depositor

Axel Brunger

Article

Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

Insert

rat ⍺SNAP (Napa Rat)

Tags

6 his

Expression

Bacterial

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTTH+mEGFP-H1.4

Plasmid

#157799

Purpose

Expression of mEGFP and human linker histone H1.4 fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-H1.4 (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pWaldo-GFPe_PepTSo

Plasmid

#58334

Purpose

Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

Depositor

So Iwata, Simon Newstead

Article

Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

Insert

PepTso

Tags

GFP-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pOSV807

Plasmid

#126600

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Article

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTTH+H1.4ΔCTD

Plasmid

#157797

Purpose

Expression of human linker histone H1.4 without c-terminal tail

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H1.4ΔCTD (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pOSV811

Plasmid

#126604

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Article

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge