30,882 results

1. pMBP-MS2

   Plasmid

   #65104

   Purpose

   can be used to isolate RNA binding complexes

   Depositor

   Josep Vilardell

   Article

   Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

   Insert

   MS2
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   double mutation (V75Q and A81G) that prevents oli…

   Promoter

   tac

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBBR15.2-2H12.D11opt-scarRef

   Plasmid

   #221150

   Purpose

   cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Inserts

   cdGreen2.1
   mScarlet-I
   

   Expression

   Bacterial

   Mutation

   N-terminus changed: starts with MSKKYGEAVIKE ...

   Promoter

   Constitutive and None (same as cdGreen2.1)

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-Int

   Plasmid

   #50000

   Purpose

   Expresses the integrase on a suicide plasmid

   Depositor

   Brian Robertson, Peter Sander

   Article

   Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

   Insert

   Integrase from pMV361
   

   Expression

   Bacterial

   Promoter

   own

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pAWP78-PVCpnf1-16

   Plasmid

   #198271

   Purpose

   Unmodified pPVC; contains WT PVC structural/accessory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf1-16
   

   Expression

   Bacterial

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GST-RBD

   Plasmid

   #15247

   Depositor

   Martin Schwartz

   Article

   Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

   Insert

   Rhotekin (Rtkn Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 7-89 (Rho binding domain)

   Available Since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBR322-PVCpnf17-22

   Plasmid

   #198272

   Purpose

   Unmodified pPayload; contains WT PVC payloads/regulatory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf17-22
   

   Expression

   Bacterial

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-Expotin 1

   Plasmid

   #42047

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   Exportin-1 (XPO1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTW335c3

   Plasmid

   #237322

   Purpose

   expresses A. thaliana RbcL RbcS-6xHis

   Depositor

   Tina Wang

   Article

   Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

   Inserts

   A. thaliana RbcL (rbcL Mustard Weed)
   A. thaliana RbcS-6xHis
   

   Tags

   6xHis and None

   Expression

   Bacterial

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15bpATnp

   Plasmid

   #121137

   Purpose

   This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

   Depositor

   Keji Zhao

   Article

   Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

   Insert

   Protein A-Tnp
   

   Tags

   his

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. HMA-APOBEC1-Nb

    Plasmid

    #226012

    Purpose

    For expression of His-MBP-APOBEC1-Nanobody protein in E.coli

    Depositor

    Eugene Yeo

    Article

    Liang et al Nat Commun. 2024 Aug 16;15(1):7067. doi:

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Tags

    His-MBP and antiRabbit-nanobody

    Expression

    Bacterial

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTW334e

    Plasmid

    #237315

    Purpose

    expresses A. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2

    Depositor

    Tina Wang

    Article

    Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

    Insert

    A. thaliana Cpn60α Cpn60β Cpn20 Raf1 Raf2 RbcX Bsd2
    

    Expression

    Bacterial

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pERM2-nhpSer

    Plasmid

    #201922

    Purpose

    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

    Depositor

    Richard Cooley, Ryan Mehl

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Inserts

    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    E412P, E414F, T417K, P495M, I496W, F529S and H67R…

    Promoter

    GlnS (constitutive), OXB20 (constitutive), lpp (c…

    Available Since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMV306DIhsp+LuxG13

    Plasmid

    #49999

    Purpose

    Integrase removed to increase stability of the plasmid in mycobacteria

    Depositor

    Brian Robertson

    Article

    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

    Insert

    Bacterial luciferase operon + G13 promoter
    

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Promoter

    G13 from M. marinum G13 promoter driving luxC

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. lox2272 Neo* lox2272

    Plasmid

    #168769

    Purpose

    lox2272 (lox variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

    Depositor

    Mario Capecchi

    Insert

    Neo*
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSFDUET-1_VNp-anti-GFP mAb

    Plasmid

    #214955

    Purpose

    Bacterial expression of anti-GFP mAb light and heavy chain VNp fusions.

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-GFP mAb heavy chain
    VNp-anti-GFP mAb light chain
    

    Tags

    VNp6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-Ran(Q69L)

    Plasmid

    #42048

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    Ran (RAN Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Q69L

    Available Since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSPIH6

    Plasmid

    #190676

    Purpose

    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)

    Depositor

    John Vederas

    Article

    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi

    Inserts

    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    

    Tags

    Chitin binding domain, His tag, and N-terminal Hi…

    Expression

    Bacterial

    Mutation

    Added C-terminal his tag and None

    Promoter

    T7

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAWP78-PVCpnf_Δpvc10

    Plasmid

    #198448

    Purpose

    Produces PVCs containing truncated spike tip protein (Pvc10).

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_Δpvc10
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQE30-roGFP1-iR

    Plasmid

    #83314

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

    Insert

    roGFP1-iR
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

    Promoter

    T5

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pETM11-6xHis-TEV-hORF1p

    Plasmid

    #202589

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Kathleen Burns, Liam Holt

    Article

    Taylor et al Cell. 2013 Nov 21;155(5):1034-48. doi: 1

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Available Since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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