31,016 results

1. pGEX-5X-1-LARP7

   Plasmid

   #113545

   Purpose

   Produces an N-terminal GST-tagged full length LARP7 protein.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   LARP7 (LARP7 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKAR2-Br512

   Plasmid

   #161875

   Purpose

   Expresses Br512 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Maranhao et al medRxiv

   Insert

   Modified DNA Polymerase I Large Fragment (Br512)
   

   Tags

   8x His tag; Villin Headpiece 47 amino acid (HP47)…

   Expression

   Bacterial

   Promoter

   T7, tetPA

   Available Since

   Nov. 11, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pACYC-Skp-TF

   Plasmid

   #83921

   Purpose

   Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Skp
   Trigger Factor
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Integrin Beta1-mPA-GFP

   Plasmid

   #186302

   Purpose

   Expresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoter

   Depositor

   Pakorn Kanchanawong

   Article

   Jain et al Nat Protoc. 2022 Jul 27. pii: 10.1038/s4

   Insert

   Integrin Beta 1 (ITGB1 Human)
   

   Tags

   mPA-GFP

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEMG-GRIS

   Plasmid

   #225134

   Purpose

   For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.

   Depositor

   Lars Blank

   Article

   Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. SPLICSFAST-ER-MT-P2A

   Plasmid

   #214892

   Purpose

   Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-ER-MT-P2A
   

   Tags

   none

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOlyA(WT)

   Plasmid

   #132391

   Purpose

   Ostreolysin A for sensing sphingomyelin/cholesterol complexes

   Depositor

   Arun Radhakrishnan

   Article

   Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

   Insert

   Ostreolysin A wild-type
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: C62S, C94S, S151C

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

   Plasmid

   #119945

   Purpose

   Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

   Depositor

   Jeffrey Chao

   Article

   Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   Renilla luciferase
   

   Tags

   24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

   Expression

   Bacterial and Mammalian

   Promoter

   TetCMV

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p2CT-His-MBP-Lwa_Cas13a_WT

   Plasmid

   #91865

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LwaCas13a
   

   Use

   CRISPR

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFW2500

    Plasmid

    #224213

    Purpose

    Bacteroides genomic editing tool; IPTG inducible-Fncpf1 and recT

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Insert

    Fncpf1 and recT
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-scFv-T

    Plasmid

    #67843

    Purpose

    pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

    Depositor

    Joanna Bereta

    Article

    Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

    Insert

    antibody 1D8 light kappa chain
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBiex-1 GFPNanobody_SNAP

    Plasmid

    #82711

    Purpose

    Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

    Insert

    GFP Nanobody
    

    Tags

    6xHis Purification tag, FLAG purification tag, an…

    Expression

    Bacterial and Insect

    Promoter

    T7 Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. T777T

    Plasmid

    #113082

    Purpose

    Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    RNAi

    Expression

    Bacterial

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET15b_Bst-LF

    Plasmid

    #153313

    Purpose

    Bacterial expression of the Bst Large Fragment

    Depositor

    Drew Endy, Philippa Marrack

    Insert

    Bst Large Fragment
    

    Expression

    Bacterial

    Available Since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA

    Plasmid

    #164615

    Purpose

    Expresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.

    Depositor

    Jeffrey Chao

    Article

    Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.

    Insert

    Renilla luciferase
    

    Tags

    24xMS2v5 (3'UTR) and SunTag

    Expression

    Bacterial and Mammalian

    Promoter

    TetCMV

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

    Plasmid

    #117728

    Purpose

    Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Insert

    NanoLuc
    

    Use

    Luciferase

    Expression

    Bacterial

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJAB988

    Plasmid

    #133920

    Purpose

    miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter

    Depositor

    Christopher Voigt

    Article

    Brophy et al Nat Microbiol. 2018 Sep;3(9):1043-1053.

    Insert

    miniICE-tet(M)cat(PC194)_PT7-GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pALOD4

    Plasmid

    #111026

    Purpose

    Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A

    Depositor

    Arun Radhakrishnan

    Article

    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

    Insert

    Domain 4 of Anthrolysin O
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: S404C, C472A

    Promoter

    T7

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZA31-sulA-GFP

    Plasmid

    #78493

    Purpose

    Expression of GFP under the sulA promoter.

    Depositor

    David Bikard

    Article

    Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

    Insert

    GFPmut2
    

    Expression

    Bacterial

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-5X-1-Full-Length-MePCE

    Plasmid

    #113539

    Purpose

    Produces an N-terminal GST-tagged full length MePCE protein.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    MePCE (MEPCE Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge