30,888 results

1. pEvol-pAcFRS.2.t1

   Plasmid

   #73544

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAcFRS.2.t1
   pAcFRS.2.t1
   

   Expression

   Bacterial

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF4Ag NanoLuc (ATG-42)

   Plasmid

   #137777

   Purpose

   Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

   Depositor

   Lance Encell

   Article

   Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

   Insert

   NanoLuc (Nluc)
   

   Expression

   Bacterial and Mammalian

   Mutation

   NanoLuc contains 16 amino acid changes compared t…

   Promoter

   T7 and CMV

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPR-PsppA-mCherry

   Plasmid

   #225428

   Purpose

   Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

   Depositor

   Geir Mathiesen

   Article

   Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

   Insert

   mCherry, Cas9, SgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PsppA upstream of mCherry and Cas9, P3 upstream o…

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPROEX Aqua

   Plasmid

   #42889

   Depositor

   Fabienne Merola

   Article

   Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

   Insert

   Aquamarine
   

   Tags

   6xHis and TEV cleavage

   Expression

   Bacterial

   Mutation

   T65S and H148G

   Available Since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28c-F30-Broccoli

   Plasmid

   #66788

   Purpose

   Expresses Broccoli in the F30 scaffold in bacterial cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

   Insert

   Broccoli in the F30 scaffold
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. 10xHis-3C-BRD4(42-459) BD1-BD2

   Plasmid

   #233886

   Purpose

   pRSF-DUET1 for protein overexpression of human BRD4

   Depositor

   Alessio Ciulli

   Article

   Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

   Insert

   BRD4 (BRD4 Human)
   

   Tags

   10x his 3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBJ2

   Plasmid

   #167131

   Purpose

   A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   Kanamycin promoter

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRE118

   Plasmid

   #43830

   Depositor

   Dieter Schifferli

   Article

   Edwards et al Gene. 1998 Jan 30;207(2):149-57.

   Type

   Empty backbone

   Use

   Bacterial allelic exchange vector with sacb1

   Expression

   Bacterial

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL103-SpCas9-FLAG-3XNLS

   Plasmid

   #182032

   Purpose

   Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP

   Depositor

   Steven Lin

   Article

   Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do

   Insert

   Streptococcus pyogenes cas9 (cas9 Synthetic)
   

   Use

   CRISPR

   Tags

   12X His tag, 3XNLS, FLAG, and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET303_sumocodo_GFP_codoopti

    Plasmid

    #210393

    Purpose

    Express SUMO-GFP in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    GFP
    

    Tags

    SUMO-tag

    Expression

    Bacterial

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAD-sfGFP(Sp)-Pman

    Plasmid

    #133833

    Purpose

    Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    sfGFP(Sp)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Expression

    Bacterial

    Promoter

    Pman

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

    Plasmid

    #35152

    Depositor

    Jay Keasling, Taek Soon Lee

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    R364S in Mevalonate Kinase

    Promoter

    LacUV5, pTrc

    Available Since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDMS197

    Plasmid

    #43831

    Depositor

    Dieter Schifferli

    Article

    Edwards et al Gene. 1998 Jan 30;207(2):149-57.

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb1

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIRCgp41-1

    Plasmid

    #74227

    Purpose

    Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    gp41-1 C-intein and gp41-1 N-intein

    Expression

    Bacterial

    Promoter

    T7-lac

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFEndoNA2

    Plasmid

    #174667

    Purpose

    Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

    Depositor

    Jukka Finne

    Article

    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

    Insert

    GFP-endoNA2 fusion protein
    

    Tags

    Histag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTOX8

    Plasmid

    #127453

    Purpose

    Encodes MqsR toxin and tsPurple chromoprotein. CAM-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Inserts

    mqsR toxin
    tsPurple chromoprotein
    

    Expression

    Bacterial

    Mutation

    Codon optimized from protein sequence in FPbase

    Promoter

    apFAB46 and pRha

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. RepX_pET28a(+)

    Plasmid

    #172199

    Purpose

    Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

    Depositor

    Taekjip Ha

    Article

    Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

    Insert

    RepX superhelicase
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    C18L, C43S, C167V, S400C, C612A

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. His-GST-TEV-BRD4(42-459) BD1-BD2

    Plasmid

    #233887

    Purpose

    pGex for protein overexpression of human BRD4

    Depositor

    Alessio Ciulli

    Article

    Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

    Insert

    BRD4 (BRD4 Human)
    

    Tags

    His-GST-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDB179-Z1-PETase

    Plasmid

    #218703

    Purpose

    Encodes codon optimized Z1-PETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    Z1-PETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRE107

    Plasmid

    #43829

    Depositor

    Dieter Schifferli

    Article

    Edwards et al Gene. 1998 Jan 30;207(2):149-57.

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb1

    Expression

    Bacterial

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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