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pCas9-CR4

Plasmid

#62655

Purpose

Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

Depositor

Kristala Prather

Article

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

Cas9 nuclease

Expression

Bacterial

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pSL1521 (pSPIN, pSC101* backbone)

Plasmid

#160729

Purpose

Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCA24N-p50-ligase

Plasmid

#87742

Purpose

IPTG-inducible expression of p50-ligase fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and human p50/NF-kB (amino acids 40-366)

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf1-16

Plasmid

#198271

Purpose

Unmodified pPVC; contains WT PVC structural/accessory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf1-16

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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prhaBAD-pA-Hia5

Plasmid

#222303

Purpose

Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

Depositor

Aaron Straight

Article

Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

Insert

pA-Hia5

Tags

6His and protein A

Expression

Bacterial

Mutation

codon optimized for bacterial expression

Promoter

rhaBAD

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLp_3050sNuc

Plasmid

#122030

Purpose

Plasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakei

Depositor

Vincent Eijsink, Geir Mathiesen

Article

Mathiesen et al BMC Genomics. 2009 Sep 10;10:425. doi: 1

Insert

signal peptide Lp_3050

Expression

Bacterial

Promoter

sppA

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis T4 DNA Ligase

Plasmid

#206207

Purpose

Inducible expression of T4 DNA ligase

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

T4 DNA ligase

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET mCherry LIC cloning vector (u-mCherry)

Plasmid

#29769

Depositor

Scott Gradia

Type

Empty backbone

Tags

mCherry

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pCA24N-sso7d-ligase

Plasmid

#87745

Purpose

IPTG-inducible expression of sso7d-ligase fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and codon optimized sso7d (Sulfolobus solfa…

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-αTAT1[2-236]

Plasmid

#27101

Depositor

Maxence Nachury

Article

Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

αTubulin K40 acetyltransferase (ATAT1 Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pET28c-F30-Broccoli

Plasmid

#66788

Purpose

Expresses Broccoli in the F30 scaffold in bacterial cells

Depositor

Samie Jaffrey

Article

Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

Insert

Broccoli in the F30 scaffold

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pTD103luxI_sfGFP

Plasmid

#48885

Purpose

Positive feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

LuxI, sfGFP, and LuxR

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pH2B_mCherry_IRES_puro2

Plasmid

#21045

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pH2B (H2BC16P Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pMSP3545

Plasmid

#46888

Purpose

Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

Depositor

Gary Dunny

Article

Bryan et al Plasmid. 2000 Sep;44(2):183-90.

Type

Empty backbone

Use

Nisin-inducible expression; gram-positive bacteri…

Expression

Bacterial

Promoter

PnisA

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pMSP1D1-Strep-II

Plasmid

#224209

Purpose

Membrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.

Depositor

Anne Robinson

Article

Pettersen et al Biophys J. 2025 Jan 21;124(2):440-457. d

Insert

MSP1D1-Strep-II

Tags

Strep-II Tag

Expression

Bacterial

Mutation

Substitution of 7xHis tag found in pMSP1D1 for a …

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pETM6-HpaBC

Plasmid

#71260

Purpose

Expresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6

Depositor

Mattheos Koffas

Article

Jones et al Biotechnol Prog. 2015 Oct 21. doi: 10.10

Inserts

HpaB
HpaC

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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pNCST-avGFP

Plasmid

#129508

Purpose

Expresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

avGFP

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_RAPGEF4_DEP

Plasmid

#109895

Purpose

Protein expression and purification of RAPGEF4_DEP

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RAPGEF4_DEP (RAPGEF4 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Glt1-eGFP

Plasmid

#176862

Purpose

Expresses Glt-1 fused with GFP at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Glt1-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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SIRT1.1

Plasmid

#13735

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

SIRT1 (SIRT1 Human)

Tags

His

Expression

Bacterial

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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