30,904 results

1. pMTTH+H1.4

   Plasmid

   #157796

   Purpose

   Expression of human linker histone H1.4

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   H1.4 (H1-4 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   pTAC

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AP1

   Plasmid

   #179678

   Purpose

   Accessory plasmids

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   [SD8] gIII
   

   Expression

   Bacterial

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKD3_mCherry

   Plasmid

   #187385

   Purpose

   To chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRT

   Depositor

   Roi Avraham

   Insert

   mCherry
   

   Expression

   Bacterial

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Human Parkin (1-465)

   Plasmid

   #110758

   Purpose

   Bacterial expression of human Parkin

   Depositor

   David Komander

   Article

   Wauer et al EMBO J. 2013 Jul 31;32(15):2099-112. doi

   Insert

   PRKN (PRKN Human)
   

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDx_mScarlet3-SYFP2

   Plasmid

   #189764

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-SYFP2
   

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MP4

   Plasmid

   #69652

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA
   

   Expression

   Bacterial

   Promoter

   E. coli PBAD

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDB016

   Plasmid

   #99220

   Purpose

   Accessory plasmid for aaRS PACE; negative selection

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   PT7 [SD8] gIII-neg
   

   Expression

   Bacterial

   Promoter

   PT7

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Beta-Lactamase

   Plasmid

   #62729

   Purpose

   Expresses Beta-Lactamase protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   Beta-Lactamase
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Deletion of periplasmic signal domain

   Promoter

   T7

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTriEx-mVenus-Zdk1

   Plasmid

   #81047

   Purpose

   mammalian expression of Zdk1

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk1
   

   Tags

   6x His tag, FLAG, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJMP3654

    Plasmid

    #222349

    Purpose

    Expression vector with GFP expressed under PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli.

    Depositor

    Jason Peters

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PabstBR

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNIC-NH10-GG

    Plasmid

    #215806

    Purpose

    Cloning of target gene with N terminal non cleavable His10 tag

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. USP5

    Plasmid

    #25299

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP5.001.835 (USP5 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 16, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pGGB_RT_Tq2CFP_li

    Plasmid

    #136965

    Purpose

    Goldengate/Greengate N-tag module, containing Tq2CFP fused to a flexible linker

    Depositor

    Klaus Theres

    Insert

    Tq2CFP
    

    Expression

    Bacterial

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EKAR2G_design1_mTFP_wt_Venus_wt

    Plasmid

    #39813

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a(+)-Gx-SB

    Plasmid

    #220125

    Purpose

    Expresses the 1.3-kDa Small BiT (SB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx).

    Depositor

    Maarten Merkx

    Article

    Ni et al Nat Commun. 2021 Jul 28;12(1):4586. doi:

    Insert

    Gx-SB
    

    Expression

    Bacterial

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a(+)-Gx-LB

    Plasmid

    #220126

    Purpose

    Expresses the 18-kDa Large BiT (LB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx).

    Depositor

    Maarten Merkx

    Article

    Ni et al Nat Commun. 2021 Jul 28;12(1):4586. doi:

    Insert

    Gx-LB
    

    Expression

    Bacterial

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNIC-NHSTIIT-GG

    Plasmid

    #215789

    Purpose

    Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pBBR1MCS-2

    Plasmid

    #85168

    Purpose

    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

    Depositor

    Kenneth Peterson

    Article

    Kovach et al Gene. 1995 Dec 1;166(1):175-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Constitutive (derepressed P-lac)

    Available Since

    Nov. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pET29b-10xHis-Super TEV

    Plasmid

    #193833

    Purpose

    Expresses high-efficiency TEV protease in bacterial cells

    Depositor

    EPFL Protein Production and Structure Core Facility (PTPSP), Florence Pojer

    Article

    Lau et al protocols.io, doi: 10.17504/protocols.io

    Insert

    TEV protease
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJL1-sfGFP

    Plasmid

    #102634

    Purpose

    In vitro expression of sfGFP from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    sfGFP
    

    Tags

    strep tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge