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pSIJ8

Plasmid

#68122

Purpose

Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

Depositor

Alex Nielsen

Article

Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

Inserts

flp recombinase
rhaS
rhaR

Expression

Bacterial

Promoter

rhamnose

Available Since

Dec. 8, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX6P1-mCherry-Nanobody

Plasmid

#70696

Purpose

Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

mCherry-Nanobody

Tags

GST

Expression

Bacterial

Available Since

March 17, 2016

Availability

Academic Institutions and Nonprofits only

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pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

Plasmid

#199390

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Tags

14xHis-Avi-SUMOEu

Expression

Bacterial

Promoter

T5-LacO

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pA-His6-Tn5

Plasmid

#171871

Purpose

Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

Depositor

Kaiwei Liang

Article

Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

Insert

Protein A and hyperactive Tn5 transposase

Tags

His-tag

Expression

Bacterial

Mutation

WT

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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human p53-(1-393)

Plasmid

#24859

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Full length.

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pET11phiC31poly(A)

Plasmid

#18942

Depositor

Michele Calos

Article

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Expression

Bacterial

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Gm-lux

Plasmid

#64963

Purpose

mini-Tn7 luxCDABE transcriptional fusion vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

lux operon

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET His6 Mocr TEV LIC cloning vector (2O-T)

Plasmid

#29710

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-Mocr-TEV

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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EGFP-pBAD

Plasmid

#54762

Purpose

Localization: Protein Expression Vector , Excitation: 488, Emission: 507

Depositor

Michael Davidson

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

6xHis and EGFP

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET28b_H6-Smt3-AesC-GFP

Plasmid

#234486

Purpose

Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

Depositor

Henning Mootz

Article

Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

Insert

C-terminal Fragment of the Aes123 PolB1 Split Intein

Tags

Hexahistidine Tag, Superfolder Green Fluorescent …

Expression

Bacterial

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMAL-c2X_MBP-CLmN-H6

Plasmid

#234487

Purpose

Maltose binding protein N-terminally fused to His-tagged CLmN split intein

Depositor

Henning Mootz

Article

Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

Insert

N-terminal Fragment of the CLm Split Intein

Tags

Hexahistidine Tag and Maltose Binding Protein (MB…

Expression

Bacterial

Mutation

T69K, F75H, M118N within the N-terminal fragment …

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-SUMO-MarathonRT

Plasmid

#109029

Purpose

Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

Depositor

Anna Pyle

Article

Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

Insert

ERE_01490

Tags

6xHis-SUMO tag

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET3a-hUBA1

Plasmid

#63571

Purpose

Expression of human UBA1 tagged with His

Depositor

Titia Sixma

Article

Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

Insert

UBA1 (UBA1 Human)

Expression

Bacterial

Mutation

Isoform 2 (1018 amino acids)

Promoter

T7

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJOE7706.1

Plasmid

#135075

Purpose

A shuttle vector for heterologous HA production with Corynebacterium glutamicum

Depositor

Josef Altenbuchner

Article

Hoffmann et al J Appl Microbiol. 2014 Sep;117(3):663-78

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pE-SUMO CRYGS

Plasmid

#80753

Purpose

Expression of human gamma S-crystallin in E. coli

Depositor

Larry David

Article

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYGS (CRYGS Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pEvolvR-enCas9-PolI3M-TBD

Plasmid

#113077

Purpose

Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber, David Schaffer

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI3M-TBD

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET-Bxb1-His

Plasmid

#123132

Purpose

Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Bxb1 integrase

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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BirA pLEMO

Plasmid

#119818

Purpose

Express endogenous biotin ligase in E.coli

Depositor

Alexander Gabibov

Article

Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

Insert

Biotin ligase

Expression

Bacterial

Promoter

Rhamnose promoter

Available Since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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pTECH-chAcK3RS(IPYE)

Plasmid

#104069

Purpose

Expression plasmid encoding chAcK3RS(IPYE) and pylT

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp chAcK3RS(IPYE); PproK pyl

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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