32,161 results
-
Plasmid#44566Purposefor generation of knockout of mycobacterial genesDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorExpressionBacterialAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only
-
pJKR-H-cdaR
Plasmid#62557PurposeGlucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.DepositorInsertsbeta lactamase
sfGFP
CdaR
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-Smurf1 wt
Plasmid#13502DepositorAvailable SinceDec. 4, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-HisYFP-Sp100
Plasmid#53141PurposeE.coli expression of HisYFP tagged human Sp100 fragmentDepositorInsertSp100A fragment (SP100 Human)
TagsHis-YFPExpressionBacterialMutationfragment (amino acids 71-480); deletion of L75PromoterT7Available SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG8Ha-EWS-FLI1_Type2
Plasmid#188042PurposeExpresses His8-EWS-FLI1(TypeII) with a TEV site for removal of the His8 tag.DepositorAvailable SinceJune 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCasY1
Plasmid#87687Purposep15A plasmid expressing CasY1DepositorInsertCasY1
ExpressionBacterialAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192789PurposeaGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody minimizer
TagsH6 and ybbRExpressionBacterialAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbE5a-Opto-Cre-Vvd
Plasmid#134404PurposeLight-inducible Cre recombinase split with Vivid photodimers for bacterial expression.DepositorInsertOpto-Cre-Vvd
UseCre/Lox and Synthetic BiologyExpressionBacterialPromoterlacUV5Available SinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm_P1T_DP-GH023 - NanoLuc
Plasmid#117727PurposeIntegration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023 upstream of the NanoLuc reporterDepositorInsertNanoLuc
UseLuciferaseExpressionBacterialAvailable SinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD18HMGR
Plasmid#19041DepositorInserttruncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase (HMG1 Budding Yeast)
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huErCas12a
Plasmid#174685PurposeExpresses 6xHis-MBP tagged ErCas12aDepositorArticleInserthuErCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 6xHisExpressionBacterialMutationQ926R mutation is observed as indicated from the …PromoterT7Available SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
RBD#3L
Plasmid#193020PurposehnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13aDepositorInsertCas13a
TagshnRNP A1 RRM2ExpressionBacterialAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSC234
Plasmid#216357PurposexhNup93-Nb3t (+2Cys) Nanobody productionDepositorInsertxhNup93-Nb3t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETCON-PTX-S1-220
Plasmid#67747PurposePertussis Toxin Subunit 1 Yeast Display PlasmidDepositorInsertPTxS1-220
ExpressionBacterial and YeastAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-163
Plasmid#118542PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1
Plasmid#122084PurposeExpress M.EcoGII fused to TRF1 with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-TERF1
UseRetroviralTagsV5 tag on Nter of TERF1ExpressionBacterial and MammalianAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRBV5-1
Plasmid#123414Purposeconstruction of TCRDepositorAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-TAX1BP1
Plasmid#171423PurposeFor the purifcation of TAX1BP1 proteinDepositorInsertTAX1BP1 (TAX1BP1 Human)
ExpressionBacterialAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET_MBP-RGG-RGG
Plasmid#124946PurposeExpression of MBP-RGG-RGGDepositorAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB3-eGFP-6xHis
Plasmid#130983PurposeBacterial expression of mouse EB3-GFP-6xHis for protein purification.DepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-mCherryBb
Plasmid#118230PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherryDepositorInsertflagellin promoter driven mCherry
ExpressionBacterialAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mClover3
Plasmid#133453PurposeExpresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mClover3
TagsGSGSGSGS linker and His6ExpressionBacterialPromoterT7Available SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 2, MG-26-18)
Plasmid#110744PurposeBacterial expression of human USP30 (construct 2)DepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT119b
Plasmid#50551PurposeExpresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoterDepositorInsertsCcaS
CcaR
sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
TS246_pNKI-2-13_HALO-DPN7
Plasmid#200720Purposeproducing Methyl Tracer HALO protein in bacteriaDepositorInsertHALO-DPN7
Tags6xHisExpressionBacterialMutationcodon optimizedAvailable SinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHEA
Plasmid#168297PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
Tags6xHis, E-tag, and PelB signal peptideExpressionBacterialPromoterLacI-PlacAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-hXRCC1
Plasmid#52274PurposeBacterial expression of human XRCC1 with N-terminal GST-tagDepositorAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB3-Flag PLK1 T210D
Plasmid#68272Purposebacterial expression of Plk1 T210D mutantDepositorInsertPLK1 (PLK1 Human)
TagsFlag and Mxe intein/chitin binding domainExpressionBacterialMutationT210DPromoterT7Available SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
pJBL3752
Plasmid#128809PurposeFluoride riboswitch-regulated sfGFPDepositorInsertFluoride riboswitch-regulated sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only